General information for g2211.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Nedd8-activating enzyme E1 catalytic subunit.
Parent gene
- The parent gene g2211 is located on chr_3: 16025800..16026718 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2211 | g2211.t5 | TTS | g2211.t5 | 16025230 | 16025230 |
| chr_3 | g2211 | g2211.t5 | isoform | g2211.t5 | 16025800 | 16026718 |
| chr_3 | g2211 | g2211.t5 | exon | g2211.t5.exon1 | 16025800 | 16026525 |
| chr_3 | g2211 | g2211.t5 | cds | g2211.t5.CDS1 | 16025801 | 16026525 |
| chr_3 | g2211 | g2211.t5 | exon | g2211.t5.exon2 | 16026577 | 16026718 |
| chr_3 | g2211 | g2211.t5 | cds | g2211.t5.CDS2 | 16026577 | 16026718 |
| chr_3 | g2211 | g2211.t5 | TSS | g2211.t5 | 16026776 | 16026776 |
Sequences
>g2211.t5 Gene=g2211 Length=868
ATGGTTGAAACAGATTTAAGCAAGAAATGGAATTATTTAAGGAAAATATTAGAGCGTTCC
GGTCCATTTGACACTGAAGCTTTTACGCCATCGTCAGATACTCTAGATTTTCTTCATAAT
ACTTGCAAAATTTTAGTCATTGGTGCCGGTGGATTGGGTTGTGAATTGCTTAAAGATCTA
GCAATGATGGGATTCAAAAATATTGATGTCATTGATATGGATACAATTGAACTCTCAAAT
TTGAATCGACAATTTTTGTTTAGAAGAAAAGACATTGGAAAATCAAAAGCAGAAACTGCT
GCAGAATTTATTAACAAAAGAATTCCAGGAACAAAAGTTACACCGCACTTTTGTAAAATT
CAAGACTATGATGAGTCATTTTATAGACAATTCCATATAGTTGTGAGCGGTTTAGATTCT
ATCGTAGCACGTCGATGGATCAATGGAATGCTAATTTCTCTTGTAAGGCTTGAAGAAGAT
GGCACAGTTGATCAGTCATCTGTTATTCCTATGATTGATGGAGGAACTGAAGGATTCAAA
GGAAATGCTCGAGTAATTTTTCCTACTCTCAATGCTTGTATTGATTGCAATCTTGATCTC
TTTCCACCTCAAATTTCATATCCTCTTTGTACTATTGCTAATACTCCTCGCTTACCAGAG
CATTGCATTGAATATGTTAAAATAATTCAATGGGACAAAGAAAATCCATTTAAATGTGCT
CTTGATGGAGATGACCCTGAACATGTGAGTTGGGTATATGAAAAAGCTCAAGAGCGTGCA
AATATTTATAATATCACTGGCCTCTCCTATCGTTTGGTTCAAGGAGTACTCAAGAATATT
ATTCCTGCTGTATCATCAACTAATGCCG
>g2211.t5 Gene=g2211 Length=289
MVETDLSKKWNYLRKILERSGPFDTEAFTPSSDTLDFLHNTCKILVIGAGGLGCELLKDL
AMMGFKNIDVIDMDTIELSNLNRQFLFRRKDIGKSKAETAAEFINKRIPGTKVTPHFCKI
QDYDESFYRQFHIVVSGLDSIVARRWINGMLISLVRLEEDGTVDQSSVIPMIDGGTEGFK
GNARVIFPTLNACIDCNLDLFPPQISYPLCTIANTPRLPEHCIEYVKIIQWDKENPFKCA
LDGDDPEHVSWVYEKAQERANIYNITGLSYRLVQGVLKNIIPAVSSTNA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2211.t5 | Gene3D | G3DSA:3.40.50.720 | - | 28 | 289 | 3.8E-129 |
| 7 | g2211.t5 | Gene3D | G3DSA:1.10.10.520 | Ubiquitin activating enzymes (Uba3). Chain: B | 206 | 280 | 3.8E-129 |
| 2 | g2211.t5 | PANTHER | PTHR10953 | UBIQUITIN-ACTIVATING ENZYME E1 | 5 | 289 | 8.8E-128 |
| 3 | g2211.t5 | PANTHER | PTHR10953:SF6 | NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT | 5 | 289 | 8.8E-128 |
| 1 | g2211.t5 | Pfam | PF00899 | ThiF family | 40 | 289 | 2.6E-56 |
| 5 | g2211.t5 | ProSitePatterns | PS00865 | Ubiquitin-activating enzyme active site. | 208 | 216 | - |
| 4 | g2211.t5 | SUPERFAMILY | SSF69572 | Activating enzymes of the ubiquitin-like proteins | 41 | 289 | 5.36E-84 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008641 | ubiquitin-like modifier activating enzyme activity | MF |
| GO:0045116 | protein neddylation | BP |
| GO:0019781 | NEDD8 activating enzyme activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
