General information for g2218.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Venom dipeptidyl peptidase 4.
Parent gene
- The parent gene g2218 is located on chr_3: 16060166..16060938 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2218 | g2218.t1 | TSS | g2218.t1 | 16055866 | 16055866 |
| chr_3 | g2218 | g2218.t1 | isoform | g2218.t1 | 16056177 | 16060651 |
| chr_3 | g2218 | g2218.t1 | exon | g2218.t1.exon1 | 16056177 | 16056401 |
| chr_3 | g2218 | g2218.t1 | cds | g2218.t1.CDS1 | 16056177 | 16056401 |
| chr_3 | g2218 | g2218.t1 | exon | g2218.t1.exon2 | 16058211 | 16058447 |
| chr_3 | g2218 | g2218.t1 | cds | g2218.t1.CDS2 | 16058211 | 16058447 |
| chr_3 | g2218 | g2218.t1 | exon | g2218.t1.exon3 | 16058507 | 16060175 |
| chr_3 | g2218 | g2218.t1 | cds | g2218.t1.CDS3 | 16058507 | 16060175 |
| chr_3 | g2218 | g2218.t1 | exon | g2218.t1.exon4 | 16060242 | 16060651 |
| chr_3 | g2218 | g2218.t1 | cds | g2218.t1.CDS4 | 16060242 | 16060651 |
| chr_3 | g2218 | g2218.t1 | TTS | g2218.t1 | 16060899 | 16060899 |
Sequences
>g2218.t1 Gene=g2218 Length=2541
ATGTTACAAGCTCCGTCGTATAAAATACTTTCACAAGAGCTCAGTTTGAGCGCAGATGAA
AATGAAAATGAATTTAGTTTGTCGCTGAGCTCAGATGGATCACAAGACTCATTGGATTTG
GTCTTAGTTGAAATTGGAAATAAAAAACGTACTAAAACTGTGCGTCGAAAAAAATACCAT
AGAACAAAAAAAATGAATAGAATTGATGGCTTTGAATCATATGAGGAGTTGATTGGCTCA
GCAAAACGCAGAAAACAATTTATAATACTTGGCGGATTATGCTTACTTGCAGTGCTTATA
ATTGTGGCAATTGTTGTGTCACTTGTGCTTGTAAATAAAAGATCAAATGATGATAGTTTA
GAGGAAGTAGATCTTGATGACATCTTAAAGAGTAAGCTACAGCCGAGAAGATTCAATGGC
ACTTGGATCGATGATAAAAGTTTTTATTATTTTGATGAACTTGGCGATTTTATAATTTAC
GATGTTTTGTCTAAAACTAAGAAAGTAGCCGTAAATCACTCAGCGCCTGCATTTGAAAAA
TTTTTCTCTGCCTCAAGTTACGAGTTTTCTGCTGATGGAAAATATATTTTGCTTGCCACA
GAAATAAGTAAACTCTATCGTCATTCATTCCTCGCCACATGGTATTATTACAACATTGAT
GAACAATCCATAACTGAGATTAGTCTCGATAATAATCAAGACAAATCATATCGACTTGCC
AAGTTTGGTCCAGTAGGAAGTGAAATGATTGTAGTCGATAAAAACAATCTCTACTATAAG
TCAACACCTTCGGCAACAGCTGTTCCAATAACAAAAGATCCCTTTGATCCCTCATCATTT
AAATTAAATGGTGTACCTGATTGGGTATATGAGGAAGAAATTTTCTCATCGAATTCCGCC
ACTTGGTTTTCGACAGATGGCAAAAAAGTTGCATTTATTCAGTTTGATGATACGCATGTT
CCAACTATTGCATTATCACTTTATGGAGCTCCCGGGATGTTGAAATATCCACACATTATT
CCTATTCAATATCCAAAAGCAGGCGCACCAAATCCAACAGTACGACTTTTCTCTGTTGAT
TTGGCAAATTTTAATTCAGATAATTTACAATATATCACTAAACCTGCAGATATCGACGTT
GATCATTTGATCACTTCAGTGGCGTGGGCAAACGAAAATGATTTAATTTCTGTCTGGACA
AATCGAGTTCAAAATAAAGGTTTAGTTTACAAATGTAACAATACTGGTTGTGAAAATGTT
CTTACTCTTGACAAAAGTGATGGATGGATTGAATTTTACACTGAGCCTTTCTTCAATAAA
GCTGGAAATAATATGATTTTTATTGACTCAAATAACGGTTACAGACATATTCAAGTGCTC
GATTTAAACACGAAAAGAGTTGTTAGTCGTACTTCTGGAAAATTTGTAGTAACTGAAATT
TTAAAATATAACAAAGAGCATGATGTGATAATTTACACAGCAAATACTGAAGAGGATATA
AAAGCACAACATGTGTATGCTATTAAAAATGAAGACTCTGCAACGCCTGTTTGTTTGACT
TGTAATTTGCACAATGATCACACATATTATAATGCTGAAGTATCTGAAGCTGGTAATCGT
TTAGTTATAACATCATCTGGTCCTCAAATTCCACGTGTAGATTTTTATACTCTGAAAGTT
GATGACAATAAAATTGAACTTTCTGAACATTTCGAAATTGAAAATAACAATGACATGAAG
AATGTTCTCAATGGAAAAAGTTTACCAAAGAAACTTTATGATAAAATTACATTTGATGAT
GGAACAGAAGCTTATGTAATGATGCTTCTTCCTCCAAATTTGAATGAAGACAATAAATAT
CCAATGCTTGTTGAAGTTTATGGTGGTCCGGATTCATCTAGTGTTACTAATAGATTCTCT
ATTGAATGGGGAACATATTTGGCATCAAGTTTAGGTATTGTATATACGAAAATTGATGGT
CGAGGTAGTGGATTGAGAAGTGATGAGCACTTGCATAAATTGTACAAAAATCTTGGAACA
GTTGAAGTTGAAGATCAAATAAGAACTGTACAAAAACTTTTAGAAAAATATTCGTACTTA
GATGAATCACGTACCGGGATTTGGGGTTGGAGTTACGGAGGCTATGTTTCGGGTATGAGT
TTAATGAGTGACAACGATGTTTTCAAATGTGCCACAAGTGTCGCTCCAGTAGTCGATTGG
ATGCTTTATGACTCAATTTACACTGAACGTTACATGCTTACACCAGAATTAAATGAAAGA
GCTTATAATGTCTCGCGAATGACACACTTCGTTGACAACATCACGAAATATAATAAACAA
TACATGTTAGTTCATGGAACACTAGATGATAATGTACACTTCCAACAAGGCATGGTTTTA
GCAAGAGTTTTAGAGCGTAGCGACATTCAATTTAAAGAAATAACATATCCCGATGAAGAT
CATTCATTAGCTGGTGTGCGTCCACATTTGTATCATTCATTGGAGAGATTTTTCAGGCAA
TGTTTCAAATTGACTGAGTAA
>g2218.t1 Gene=g2218 Length=846
MLQAPSYKILSQELSLSADENENEFSLSLSSDGSQDSLDLVLVEIGNKKRTKTVRRKKYH
RTKKMNRIDGFESYEELIGSAKRRKQFIILGGLCLLAVLIIVAIVVSLVLVNKRSNDDSL
EEVDLDDILKSKLQPRRFNGTWIDDKSFYYFDELGDFIIYDVLSKTKKVAVNHSAPAFEK
FFSASSYEFSADGKYILLATEISKLYRHSFLATWYYYNIDEQSITEISLDNNQDKSYRLA
KFGPVGSEMIVVDKNNLYYKSTPSATAVPITKDPFDPSSFKLNGVPDWVYEEEIFSSNSA
TWFSTDGKKVAFIQFDDTHVPTIALSLYGAPGMLKYPHIIPIQYPKAGAPNPTVRLFSVD
LANFNSDNLQYITKPADIDVDHLITSVAWANENDLISVWTNRVQNKGLVYKCNNTGCENV
LTLDKSDGWIEFYTEPFFNKAGNNMIFIDSNNGYRHIQVLDLNTKRVVSRTSGKFVVTEI
LKYNKEHDVIIYTANTEEDIKAQHVYAIKNEDSATPVCLTCNLHNDHTYYNAEVSEAGNR
LVITSSGPQIPRVDFYTLKVDDNKIELSEHFEIENNNDMKNVLNGKSLPKKLYDKITFDD
GTEAYVMMLLPPNLNEDNKYPMLVEVYGGPDSSSVTNRFSIEWGTYLASSLGIVYTKIDG
RGSGLRSDEHLHKLYKNLGTVEVEDQIRTVQKLLEKYSYLDESRTGIWGWSYGGYVSGMS
LMSDNDVFKCATSVAPVVDWMLYDSIYTERYMLTPELNERAYNVSRMTHFVDNITKYNKQ
YMLVHGTLDDNVHFQQGMVLARVLERSDIQFKEITYPDEDHSLAGVRPHLYHSLERFFRQ
CFKLTE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g2218.t1 | Gene3D | G3DSA:2.140.10.30 | - | 178 | 589 | 5.0E-207 |
| 9 | g2218.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 590 | 838 | 5.0E-207 |
| 3 | g2218.t1 | PANTHER | PTHR11731 | PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED | 80 | 845 | 3.3E-250 |
| 4 | g2218.t1 | PANTHER | PTHR11731:SF192 | IP17501P | 80 | 845 | 3.3E-250 |
| 1 | g2218.t1 | Pfam | PF00930 | Dipeptidyl peptidase IV (DPP IV) N-terminal region | 190 | 551 | 5.6E-87 |
| 2 | g2218.t1 | Pfam | PF00326 | Prolyl oligopeptidase family | 641 | 843 | 9.7E-51 |
| 10 | g2218.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 86 | - |
| 12 | g2218.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 111 | - |
| 11 | g2218.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 112 | 846 | - |
| 7 | g2218.t1 | SUPERFAMILY | SSF82171 | DPP6 N-terminal domain-like | 122 | 580 | 3.66E-52 |
| 6 | g2218.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 596 | 843 | 7.67E-62 |
| 5 | g2218.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 109 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006508 | proteolysis | BP |
| GO:0008236 | serine-type peptidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01278 (dipeptidyl-peptidase 4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
