General information for g2254.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heat shock protein 90.
Parent gene
- The parent gene g2254 is located on chr_3: 16308902..16314786 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2254 | g2254.t1 | TSS | g2254.t1 | 16302050 | 16302050 |
| chr_3 | g2254 | g2254.t1 | isoform | g2254.t1 | 16302197 | 16309500 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon1 | 16302197 | 16302328 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS1 | 16302197 | 16302328 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon2 | 16302388 | 16302518 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS2 | 16302388 | 16302518 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon3 | 16302574 | 16302784 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS3 | 16302574 | 16302784 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon4 | 16302898 | 16302930 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS4 | 16302898 | 16302930 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon5 | 16303280 | 16303287 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS5 | 16303280 | 16303287 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon6 | 16303398 | 16303495 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS6 | 16303398 | 16303495 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon7 | 16304032 | 16304147 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS7 | 16304032 | 16304147 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon8 | 16304218 | 16304255 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS8 | 16304218 | 16304255 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon9 | 16306986 | 16307013 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS9 | 16306986 | 16307013 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon10 | 16307293 | 16308844 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS10 | 16307293 | 16308844 |
| chr_3 | g2254 | g2254.t1 | exon | g2254.t1.exon11 | 16308908 | 16309500 |
| chr_3 | g2254 | g2254.t1 | cds | g2254.t1.CDS11 | 16308908 | 16309500 |
| chr_3 | g2254 | g2254.t1 | TTS | g2254.t1 | 16309725 | 16309725 |
Sequences
>g2254.t1 Gene=g2254 Length=2940
ATGGAAAAGTGGGAAGGAAAAGTAGCTGTTGTCACTGGTGCTTCTGTTGGAATAGGGGCC
GCTTTGTGTCGTGCACTTGTCGAAGCTGGAATGATCGTTTGTGGATTATCAAAACGCAAG
GATAAAATGGAAGCTATTCGACGAACACTTTTTGGTGTTAAAGGGCAATTTAATTGCGTT
GAATGTGACATTCGTGATGAAAATCAAGTCGCTGCTGCTTTTAGATGGATTGAGGATGTT
TGTGGTGGAGTCGATTTATTGATAAACAATGCTGGTGTTATCACTAAAGGTCTTCTCTTG
GACGCAAAAAATACATCCGAACTCTACAGAACTATGGAGACTAACATCATTGGTCTCTGT
CTCATTACTCGTGAAGCTGTTAAACTTATGAAAAAGCGCGATGAAGAACGAGGAACTGTA
GGACATATTGTGAACATCAATAGCATATTCGGTCACAAGATTCATGCCTGTGTTCCGGGA
ACAAAGCCTCTAAATGGGATGTATCCGGCCTCAAAATTCGCTGTCACTGCAATCACAGAA
TGTGTCCGTCAAGAATTATTGTATTTAGGAACAAAAATCAAAATCACAAGTATTTCACCG
GGATTGGTCGAAGGTGACATTGGTACTTTAAACACTTCTCATGACATGATCAAATTGATG
CCAAAGCTTAATCCACAAGATGTTGCAGATGCAGTTCTTTATGCTATTTCTACACCAGAA
AATGTTTTGATTCACGAATTGATAATCAAACCAGTCGGAGAATTTCTTCATAAAAGCAGA
AAATCAAAAAGAAAAATGCCAGCAGAAGCAGAAGTTTTCCAATTTCAAGCTGAAATAGCT
CAGCTTATGTCACTTATTATCAACACCTTTTATTCAAACAAGGAGATTTTCTTGCGTGAA
TTGATCTCAAATTCATCTGATGCTCTCGATAAAATCCGTTATGAATCACTCACTGAACCT
TCAAAATTGGATTCAGGAAAGGAACTTTTTATTAAGATTATTCCAAACAAAGAGGCCCGT
ACTTTGACAATTATCGATACTGGTATTGGTATGACAAAGTCTGATCTCGTAAACAATCTT
GGAACAATCGCTCGCTCAGGCACAAAAGCCTTTATGGAAGCTTTACAAGCTGGCGCTGAT
ATCTCTATGATTGGTCAATTTGGTGTCGGTTTCTATTCTGCATACTTGGTTGCTGACAAA
GTCACAGTTCACTCAAAGAACAACGATGATGAGCAATATGTTTGGGAATCATCAGCTGGT
GGTAGTTTTACAATTGCAGTTGATGAAGGTGAGCCATTAGGTCGTGGTACAAAGATTGTT
TTGCACATTAAAGAAGATCAGACTGAATACTTGGAAGAGTCAAAGATCAAGTCGATTGTC
ACAAAACACTCACAATTCATTGGCTATCCAATTAAATTGCTCGTTGAAAAAGAACGCGAG
AAGGAAGTTAGTGATGATGAAGCTGAACCAGAAGAAGACAAAGAAAAGAAAGAAGGTGAT
GAGCCTAAGATTGAAGATGTTGGTGATGATGAAGATGCAGATAAGGCTGATAAGGAAAAG
AAGAAGAAAACAGTTAAAGTTAAGTATACTGAAGATGAGGAACTCAACAAAACAAAACCA
ATTTGGACACGTAACCCAGATGACATCACTGCTGAAGAATATGGTGAATTCTACAAGAGC
TTGACAAATGATTGGGAAGATCATTTGGCTGTTAAACATTTCTCAGTTGAAGGACAACTC
GAATTCCGTGCTCTTCTCTTTGTTCCACGTCGTATTCCATTTGATCTCTTTGAAGCTAAG
AAAAAGAAGAACAACATCAAATTATACGTCAGACGTGTATTTATCATGGACAACTGTGAA
GATCTTATTCCAGATTATTTGAATTTCATGAAAGGTGTTGTTGACTCAGAAGATTTGCCA
TTGAACATTTCTCGTGAAATGTTGCAACAGAACAAGATTCTTAAAGTCATCCGCAAAAAT
CTCGTTAAGAAATGCTTAGAATTATTCGAAGAATTAGCAGAAGATAAGGAAAATTACAAG
AAATTCTATAGCCAATTCAGTAAAAACTTGAAATTAGGTGTTCATGAAGATTCAACAAAT
CGCGCAAAATTGGCTGATTTACTTCGCTTCCAAACATCTGCATCAGGTGATGAAAGCTGC
TCACTTGGCGATTATGTTGGTCGCATGAAGGAGAATCAAAAATCTATTTACTTCATTACT
GGTGAGAGCAAAGAACAAGTAAGCAATTCAGCATTTGTTGAACGTGTCAAGAAACGTGGA
TTTGAAGTTGTTTACATGACTGAACCAATTGATGAATACGTCATTCAGCAACTTAAAGAA
TATCAAGGCAAACAACTTGTTTGTGTAACAAAGGAAGGTCTTGAATTGCCAGAAGATGAG
GAAGAGAAGAAGAAGCGTGAAGAAGATAAGGCCAAATTCGAAAATCTCTGCAAAGTCATG
AAATCAGTTTTAGATAACAAAGTTGAAAAAGTTATTGTATCAAATCGTTTGGTAGAATCA
CCATGCTGCATTGTCACATCTCAATATGGCTGGTCTGCTAATATGGAGCGTATCATGAAA
GCACAAGCTCTACGTGATACAACAACAATGGGTTACATGGCTGGTAAAAAACATCTTGAA
ATCAATCCAGACCATCCAATCATCGAGACATTGCGTCAAAAGGCTGAAGCCGACAAGAAC
GATAAGGCTGTTAAAGATTTGTGCATTTTACTCTTCGAGACTTCATTACTAAGCTCAGGT
TTTTCACTTGATGAACCACAAGTTCATGCTGCTAGAATTTATCGTATGATCAAACTCGGT
TTGGGAATTGATGAAGATGAACCAATGGCTACTGAAGAACCTGCTGCAGCAGCTTCATCA
GATATGCCACCACTTGTAGATGATGCTGAAGATGCTTCACATATGGAAGAAGTTGATTAA
>g2254.t1 Gene=g2254 Length=979
MEKWEGKVAVVTGASVGIGAALCRALVEAGMIVCGLSKRKDKMEAIRRTLFGVKGQFNCV
ECDIRDENQVAAAFRWIEDVCGGVDLLINNAGVITKGLLLDAKNTSELYRTMETNIIGLC
LITREAVKLMKKRDEERGTVGHIVNINSIFGHKIHACVPGTKPLNGMYPASKFAVTAITE
CVRQELLYLGTKIKITSISPGLVEGDIGTLNTSHDMIKLMPKLNPQDVADAVLYAISTPE
NVLIHELIIKPVGEFLHKSRKSKRKMPAEAEVFQFQAEIAQLMSLIINTFYSNKEIFLRE
LISNSSDALDKIRYESLTEPSKLDSGKELFIKIIPNKEARTLTIIDTGIGMTKSDLVNNL
GTIARSGTKAFMEALQAGADISMIGQFGVGFYSAYLVADKVTVHSKNNDDEQYVWESSAG
GSFTIAVDEGEPLGRGTKIVLHIKEDQTEYLEESKIKSIVTKHSQFIGYPIKLLVEKERE
KEVSDDEAEPEEDKEKKEGDEPKIEDVGDDEDADKADKEKKKKTVKVKYTEDEELNKTKP
IWTRNPDDITAEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRIPFDLFEAK
KKKNNIKLYVRRVFIMDNCEDLIPDYLNFMKGVVDSEDLPLNISREMLQQNKILKVIRKN
LVKKCLELFEELAEDKENYKKFYSQFSKNLKLGVHEDSTNRAKLADLLRFQTSASGDESC
SLGDYVGRMKENQKSIYFITGESKEQVSNSAFVERVKKRGFEVVYMTEPIDEYVIQQLKE
YQGKQLVCVTKEGLELPEDEEEKKKREEDKAKFENLCKVMKSVLDNKVEKVIVSNRLVES
PCCIVTSQYGWSANMERIMKAQALRDTTTMGYMAGKKHLEINPDHPIIETLRQKAEADKN
DKAVKDLCILLFETSLLSSGFSLDEPQVHAARIYRMIKLGLGIDEDEPMATEEPAAAASS
DMPPLVDDAEDASHMEEVD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 29 | g2254.t1 | CDD | cd16927 | HATPase_Hsp90-like | 281 | 467 | 8.21864E-113 |
| 19 | g2254.t1 | Gene3D | G3DSA:3.40.50.720 | - | 1 | 273 | 2.5E-52 |
| 21 | g2254.t1 | Gene3D | G3DSA:3.30.565.10 | - | 274 | 475 | 9.0E-104 |
| 23 | g2254.t1 | Gene3D | G3DSA:3.30.70.2140 | - | 476 | 522 | 9.0E-104 |
| 22 | g2254.t1 | Gene3D | G3DSA:3.30.230.80 | - | 536 | 704 | 2.4E-82 |
| 18 | g2254.t1 | Gene3D | G3DSA:3.40.50.11260 | - | 705 | 791 | 1.8E-35 |
| 20 | g2254.t1 | Gene3D | G3DSA:1.20.120.790 | - | 792 | 941 | 1.7E-67 |
| 6 | g2254.t1 | Hamap | MF_00505 | Chaperone protein HtpG [htpG]. | 268 | 938 | 26.918135 |
| 33 | g2254.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 478 | 523 | - |
| 32 | g2254.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 948 | 979 | - |
| 4 | g2254.t1 | PANTHER | PTHR11528:SF79 | HEAT SHOCK PROTEIN HSP 90-BETA-RELATED | 266 | 976 | 0.0 |
| 5 | g2254.t1 | PANTHER | PTHR11528 | HEAT SHOCK PROTEIN 90 FAMILY MEMBER | 266 | 976 | 0.0 |
| 11 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 271 | 291 | 5.2E-81 |
| 13 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 292 | 314 | 5.2E-81 |
| 12 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 341 | 358 | 5.2E-81 |
| 10 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 359 | 376 | 5.2E-81 |
| 7 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 384 | 406 | 5.2E-81 |
| 9 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 435 | 452 | 5.2E-81 |
| 8 | g2254.t1 | PRINTS | PR00775 | 90kDa heat shock protein signature | 453 | 471 | 5.2E-81 |
| 3 | g2254.t1 | Pfam | PF00106 | short chain dehydrogenase | 7 | 208 | 1.0E-38 |
| 2 | g2254.t1 | Pfam | PF02518 | Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase | 293 | 446 | 5.6E-14 |
| 1 | g2254.t1 | Pfam | PF00183 | Hsp90 protein | 449 | 954 | 2.7E-231 |
| 25 | g2254.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 25 | - |
| 26 | g2254.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 27 | g2254.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 18 | - |
| 28 | g2254.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 25 | - |
| 24 | g2254.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 26 | 979 | - |
| 31 | g2254.t1 | ProSitePatterns | PS00298 | Heat shock hsp90 proteins family signature. | 291 | 300 | - |
| 30 | g2254.t1 | SMART | SM00387 | HKATPase_4 | 293 | 447 | 1.9E-8 |
| 15 | g2254.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 2 | 255 | 9.93E-52 |
| 16 | g2254.t1 | SUPERFAMILY | SSF55874 | ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase | 271 | 479 | 9.43E-74 |
| 14 | g2254.t1 | SUPERFAMILY | SSF54211 | Ribosomal protein S5 domain 2-like | 537 | 792 | 1.21E-108 |
| 17 | g2254.t1 | SUPERFAMILY | SSF110942 | HSP90 C-terminal domain | 815 | 937 | 1.31E-38 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006457 | protein folding | BP |
| GO:0005524 | ATP binding | MF |
| GO:0051082 | unfolded protein binding | MF |
| GO:0055114 | NA | NA |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04079 (molecular chaperone HtpG)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
