General information for g2254.t23
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Heat shock protein 83.
Parent gene
- The parent gene g2254 is located on chr_3: 16308902..16314786 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2254 | g2254.t23 | TSS | g2254.t23 | 16306870 | 16306870 |
| chr_3 | g2254 | g2254.t23 | isoform | g2254.t23 | 16307707 | 16308837 |
| chr_3 | g2254 | g2254.t23 | exon | g2254.t23.exon1 | 16307707 | 16308574 |
| chr_3 | g2254 | g2254.t23 | cds | g2254.t23.CDS1 | 16308343 | 16308574 |
| chr_3 | g2254 | g2254.t23 | exon | g2254.t23.exon2 | 16308644 | 16308837 |
| chr_3 | g2254 | g2254.t23 | cds | g2254.t23.CDS2 | 16308644 | 16308837 |
| chr_3 | g2254 | g2254.t23 | TTS | g2254.t23 | 16309725 | 16309725 |
Sequences
>g2254.t23 Gene=g2254 Length=1062
CACTCAAAGAACAACGATGATGAGCAATATGTTTGGGAATCATCAGCTGGTGGTAGTTTT
ACAATTGCAGTTGATGAAGGTGAGCCATTAGGTCGTGGTACAAAGATTGTTTTGCACATT
AAAGAAGATCAGACTGAATACTTGGAAGAGTCAAAGATCAAGTCGATTGTCACAAAACAC
TCACAATTCATTGGCTATCCAATTAAATTGCTCGTTGAAAAAGAACGCGAGAAGGAAGTT
AGTGATGATGAAGCTGAACCAGAAGAAGACAAAGAAAAGAAAGAAGGTGATGAGCCTAAG
ATTGAAGATGTTGGTGATGATGAAGATGCAGATAAGGCTGATAAGGAAAAGAAGAAGAAA
ACAGTTAAAGTTAAGTATACTGAAGATGAGGAACTCAACAAAACAAAACCAATTTGGACA
CGTAACCCAGATGACATCACTGCTGAAGAATATGGTGAATTCTACAAGAGCTTGACAAAT
GATTGGGAAGATCATTTGGCTGTTAAACATTTCTCAGTTGAAGGACAACTCGAATTCCGT
GCTCTTCTCTTTGTTCCACGTCGTATTCCATTTGATCTCTTTGAAGCTAAGAAAAAGAAG
AACAACATCAAATTATACGTCAGACGTGTATTTATCATGGACAACTGTGAAGATCTTATT
CCAGATTATTTGAATTTCATGAAAGGTGTTGTTGACTCAGAAGATTTGCCATTGAACATT
TCTCGTGAAATGTTGCAACAGAACAAGATTCTTAAAGTCATCCGCAAAAATCTCGTTAAG
AAATGCTTAGAATTATTCGAAGAATTAGCAGAAGATAAGGAAAATTACAAGAAATTCTAT
AGCCAATTCAGTAAAAACTTGAAATTAGGTGATGAAAGCTGCTCACTTGGCGATTATGTT
GGTCGCATGAAGGAGAATCAAAAATCTATTTACTTCATTACTGGTGAGAGCAAAGAACAA
GTAAGCAATTCAGCATTTGTTGAACGTGTCAAGAAACGTGGATTTGAAGTTGTTTACATG
ACTGAACCAATTGATGAATACGTCATTCAGCAACTTAAAGAA
>g2254.t23 Gene=g2254 Length=142
MDNCEDLIPDYLNFMKGVVDSEDLPLNISREMLQQNKILKVIRKNLVKKCLELFEELAED
KENYKKFYSQFSKNLKLGDESCSLGDYVGRMKENQKSIYFITGESKEQVSNSAFVERVKK
RGFEVVYMTEPIDEYVIQQLKE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g2254.t23 | Gene3D | G3DSA:3.30.230.80 | - | 1 | 79 | 0 |
| 6 | g2254.t23 | Gene3D | G3DSA:3.40.50.12450 | - | 80 | 142 | 0 |
| 3 | g2254.t23 | PANTHER | PTHR11528:SF34 | HEAT SHOCK PROTEIN 83 | 1 | 142 | 0 |
| 4 | g2254.t23 | PANTHER | PTHR11528 | HEAT SHOCK PROTEIN 90 FAMILY MEMBER | 1 | 142 | 0 |
| 2 | g2254.t23 | Pfam | PF00183 | Hsp90 protein | 1 | 78 | 0 |
| 1 | g2254.t23 | Pfam | PF00183 | Hsp90 protein | 79 | 142 | 0 |
| 5 | g2254.t23 | SUPERFAMILY | SSF54211 | Ribosomal protein S5 domain 2-like | 1 | 142 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006457 | protein folding | BP |
| GO:0005524 | ATP binding | MF |
| GO:0051082 | unfolded protein binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
