Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock protein 83.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2254 g2254.t8 TSS g2254.t8 16306870 16306870
chr_3 g2254 g2254.t8 isoform g2254.t8 16306948 16307978
chr_3 g2254 g2254.t8 exon g2254.t8.exon1 16306948 16307013
chr_3 g2254 g2254.t8 exon g2254.t8.exon2 16307134 16307188
chr_3 g2254 g2254.t8 exon g2254.t8.exon3 16307293 16307978
chr_3 g2254 g2254.t8 cds g2254.t8.CDS1 16307293 16307976
chr_3 g2254 g2254.t8 TTS g2254.t8 16308052 16308052

Sequences

>g2254.t8 Gene=g2254 Length=807
TCAAAGCGATTAAAAGAATAAATAAGAGTTAATTATAGTCATAAAAGCAGAAAATCAAAA
AGAAAAAATGTGTTAAATTCAATCATTCTAATCGCGTGACTTATTTAAAAATTTCAAAAA
TATGCCAGCAGAAGCAGAAGTTTTCCAATTTCAAGCTGAAATAGCTCAGCTTATGTCACT
TATTATCAACACCTTTTATTCAAACAAGGAGATTTTCTTGCGTGAATTGATCTCAAATTC
ATCTGATGCTCTCGATAAAATCCGTTATGAATCACTCACTGAACCTTCAAAATTGGATTC
AGGAAAGGAACTTTTTATTAAGATTATTCCAAACAAAGAGGCCCGTACTTTGACAATTAT
CGATACTGGTATTGGTATGACAAAGTCTGATCTCGTAAACAATCTTGGAACAATCGCTCG
CTCAGGCACAAAAGCCTTTATGGAAGCTTTACAAGCTGGCGCTGATATCTCTATGATTGG
TCAATTTGGTGTCGGTTTCTATTCTGCATACTTGGTTGCTGACAAAGTCACAGTTCACTC
AAAGAACAACGATGATGAGCAATATGTTTGGGAATCATCAGCTGGTGGTAGTTTTACAAT
TGCAGTTGATGAAGGTGAGCCATTAGGTCGTGGTACAAAGATTGTTTTGCACATTAAAGA
AGATCAGACTGAATACTTGGAAGAGTCAAAGATCAAGTCGATTGTCACAAAACACTCACA
ATTCATTGGCTATCCAATTAAATTGCTCGTTGAAAAAGAACGCGAGAAGGAAGTTAGTGA
TGATGAAGCTGAACCAGAAGAAGACAA

>g2254.t8 Gene=g2254 Length=228
MPAEAEVFQFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTEPSKLDS
GKELFIKIIPNKEARTLTIIDTGIGMTKSDLVNNLGTIARSGTKAFMEALQAGADISMIG
QFGVGFYSAYLVADKVTVHSKNNDDEQYVWESSAGGSFTIAVDEGEPLGRGTKIVLHIKE
DQTEYLEESKIKSIVTKHSQFIGYPIKLLVEKEREKEVSDDEAEPEED

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g2254.t8 CDD cd16927 HATPase_Hsp90-like 16 202 3.26056E-117
13 g2254.t8 Gene3D G3DSA:3.30.565.10 - 6 210 3.9E-102
14 g2254.t8 Gene3D G3DSA:3.30.70.2140 - 211 226 3.9E-102
3 g2254.t8 PANTHER PTHR11528:SF79 HEAT SHOCK PROTEIN HSP 90-BETA-RELATED 4 224 2.3E-125
4 g2254.t8 PANTHER PTHR11528 HEAT SHOCK PROTEIN 90 FAMILY MEMBER 4 224 2.3E-125
11 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 6 26 5.1E-85
7 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 27 49 5.1E-85
8 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 76 93 5.1E-85
5 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 94 111 5.1E-85
6 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 119 141 5.1E-85
10 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 170 187 5.1E-85
9 g2254.t8 PRINTS PR00775 90kDa heat shock protein signature 188 206 5.1E-85
2 g2254.t8 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase 28 181 2.5E-15
1 g2254.t8 Pfam PF00183 Hsp90 protein 184 227 5.1E-11
16 g2254.t8 ProSitePatterns PS00298 Heat shock hsp90 proteins family signature. 26 35 -
17 g2254.t8 SMART SM00387 HKATPase_4 28 182 1.9E-8
12 g2254.t8 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase 6 214 2.49E-75

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006457 protein folding BP
GO:0005524 ATP binding MF
GO:0051082 unfolded protein binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values