General information for g228.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mediator of RNA polymerase II transcription subunit 17.
Parent gene
- The parent gene g228 is located on chr_3: 1877661..1883015 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g228 | g228.t8 | TTS | g228.t8 | 1877022 | 1877022 |
| chr_3 | g228 | g228.t8 | isoform | g228.t8 | 1877094 | 1883015 |
| chr_3 | g228 | g228.t8 | exon | g228.t8.exon1 | 1877094 | 1877177 |
| chr_3 | g228 | g228.t8 | cds | g228.t8.CDS1 | 1877094 | 1877177 |
| chr_3 | g228 | g228.t8 | exon | g228.t8.exon2 | 1877236 | 1878815 |
| chr_3 | g228 | g228.t8 | cds | g228.t8.CDS2 | 1877236 | 1878645 |
| chr_3 | g228 | g228.t8 | exon | g228.t8.exon3 | 1882900 | 1883015 |
| chr_3 | g228 | g228.t8 | TSS | g228.t8 | NA | NA |
Sequences
>g228.t8 Gene=g228 Length=1780
CCTAATTTTTTGATGAAATAAAATTTTTGTTATCAAAGAAAAAATATGCAAAGAAAAAAA
TAAAATTTGTAGTGTACTGATATGAAGCTGACAATCGAATGCGAATAAAAAAGAAGAAGA
ACAAACAATTCAACCTACATCAAATCCATTTGAAAATATTCTAAACAAATTGAAAGAAGC
TTATACAGAAGTTACAGTGCTTCATGATGTATTGAGTATAGCAAAGGAAAAACATTATTT
AGTTCTTGATCCAGTTCCAATTCATGAACCTATTGAAGTTAAACCAATGGCTGTTGTCTA
TGCGAGAAAAAAAGCACTTGGAATGGCGGGTAATATTTTGATATCAGGTGCAGAGAGATT
AAAAAGACTTGATAATGGTCCAGACTTTCAAATTGAATTGTTGAGATTAAGACAAAATTG
GCGCTTAAAGAAAGTAAGTGATAATATTATTGGTGATTTGAGTTTTCGAACAGCTGGATC
AAAGTTTATGCATCCTGGTAATTTTGAAGTGTCAAAAGCCGATGAATCTGAAATGAAAGG
ACCAAATCCATCAACATTATCAGTTACAATACCGTCAGATTTACAAGGAGTTGCTTATAT
AAAAGTTGTCACACAAAAAGATCAAGAAGATTTGTGCTCTGCAAACGTGAGTACAATCGA
TCAAAAATGTAAACTGCAAGTTGGTGAAATCTCTTCATGGCAAAAGAAATTTGAATTTGC
ACAAAATGTTTTATTCTGTAAAGAACTTTTTAATCAACTTGCACGTGAAGCAGTTCAGCT
TCAAGCTACGATTCCGCATGTTGTAATTGGAAATCAAATTAGAGCTACATTACTTCCAGG
CATTCAACTTATCATTAGTTTATGTCATTCAAATTCTTACCAAGAATTTCAAGAGGAGAA
TAAGAAAAAAGTTGATGATTTACACGACCATGTACTTGAGCATTCTTTGCATCAACTTCT
TCGTGAGATACATTATAAAAATGTGCATTTTCCAATTCCACATTCAGGTCATGCAGTTGG
TATTGCTAAAAAAAGATTATTGGCTGGTCCACACTCTTATGATCGTCATGAATTGTGGGA
AATTACAAAGAGTCAAAATCAGACATTACTTGAACAAATAATTGCACAAGCTCAACATGT
ATTTATGAGAAAAAGAACTCAATATGTACTCGATACTCTTGCGAGAGATGTTAAAGATCC
ACAAATCACATCACACTGGAATGCTCTTAATTCACCAACATTATCATGGGTGAAAATCAA
TATTACAACACACGGATATGATACAATTACAAGAACATCATTAGTGATTCATGTAAAAGA
AAGATCGTTAAAAGCAATTTGTAGAGATGGTCGAGTTATGCATATGTCTTATGAACCTCA
AGAACTTCGTGATTTAATTCTACAGCAAATTTGTCAGCATCAAATAAGTGGATTACAAAG
TTTGAGTCGTTGTATGGCATGGCAATTGCTATCAAATTCAAATCATTTAGGAATTGGCGA
TGTAGAACCACTTGGAAATGCAAATTCAATTTTAATTTCTTCTCCACTTGGTGATCGTGT
GATTGCAGTTCAAGTGAGATGTGATCCACAAATTGTTGTGAAAGTTTATGTTTCACACAG
TCCAAAAGAATTTTTCACAAGTGGTCCATTGGTACAAGGAAAACATTGGGAAAATCTCGG
TAGTGAATTTAAAGAGGTAAATTTCGAAAAGATGGAAGGAAGAAATTTTCTCAATAAAAT
GGAATTCTTGATGGCTGCTCTCACTAAAATTAAGGAATAA
>g228.t8 Gene=g228 Length=497
MAVVYARKKALGMAGNILISGAERLKRLDNGPDFQIELLRLRQNWRLKKVSDNIIGDLSF
RTAGSKFMHPGNFEVSKADESEMKGPNPSTLSVTIPSDLQGVAYIKVVTQKDQEDLCSAN
VSTIDQKCKLQVGEISSWQKKFEFAQNVLFCKELFNQLAREAVQLQATIPHVVIGNQIRA
TLLPGIQLIISLCHSNSYQEFQEENKKKVDDLHDHVLEHSLHQLLREIHYKNVHFPIPHS
GHAVGIAKKRLLAGPHSYDRHELWEITKSQNQTLLEQIIAQAQHVFMRKRTQYVLDTLAR
DVKDPQITSHWNALNSPTLSWVKINITTHGYDTITRTSLVIHVKERSLKAICRDGRVMHM
SYEPQELRDLILQQICQHQISGLQSLSRCMAWQLLSNSNHLGIGDVEPLGNANSILISSP
LGDRVIAVQVRCDPQIVVKVYVSHSPKEFFTSGPLVQGKHWENLGSEFKEVNFEKMEGRN
FLNKMEFLMAALTKIKE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g228.t8 | PANTHER | PTHR13114 | MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17 | 5 | 493 | 0e+00 |
| 1 | g228.t8 | Pfam | PF10156 | Subunit 17 of Mediator complex | 109 | 298 | 1e-06 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006357 | regulation of transcription by RNA polymerase II | BP |
| GO:0016592 | mediator complex | CC |
| GO:0003712 | transcription coregulator activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
