General information for g230.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Guanine deaminase.
Parent gene
- The parent gene g230 is located on chr_3: 1884600..1885778 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g230 | g230.t1 | TSS | g230.t1 | 1882416 | 1882416 |
| chr_3 | g230 | g230.t1 | isoform | g230.t1 | 1882475 | 1884600 |
| chr_3 | g230 | g230.t1 | exon | g230.t1.exon1 | 1882475 | 1882507 |
| chr_3 | g230 | g230.t1 | cds | g230.t1.CDS1 | 1882475 | 1882507 |
| chr_3 | g230 | g230.t1 | exon | g230.t1.exon2 | 1883015 | 1883131 |
| chr_3 | g230 | g230.t1 | cds | g230.t1.CDS2 | 1883015 | 1883131 |
| chr_3 | g230 | g230.t1 | exon | g230.t1.exon3 | 1883193 | 1883450 |
| chr_3 | g230 | g230.t1 | cds | g230.t1.CDS3 | 1883193 | 1883450 |
| chr_3 | g230 | g230.t1 | exon | g230.t1.exon4 | 1883514 | 1883668 |
| chr_3 | g230 | g230.t1 | cds | g230.t1.CDS4 | 1883514 | 1883668 |
| chr_3 | g230 | g230.t1 | exon | g230.t1.exon5 | 1883724 | 1884600 |
| chr_3 | g230 | g230.t1 | cds | g230.t1.CDS5 | 1883724 | 1884600 |
| chr_3 | g230 | g230.t1 | TTS | g230.t1 | 1884665 | 1884665 |
Sequences
>g230.t1 Gene=g230 Length=1440
ATGCTTTCATTTATTCTTAATCGCCAATATTCGGAAATCAGAGACGACATGGATCATCAT
GTGATTTATTTCGGAAATATTTTTCATTCAATTTCATTTAATGAATCCCAAGAAATTTTG
GATGGATTTATAGCTGTGGAAGATGGAAAAATTATAGCAATTGGAAATAAAACAGAGTTT
GAGGCGTGGAATTATGCAAACAAAGATACTTTTAAGAAGATAAATTTAACAGATACTCAA
TTTCTAATTCCTGGTTTTGTCGATTCACATATTCATGCACCACAAGTACCTAACATTGGT
TTAGGTTTAGATAAACCTTTACTTGATTGGCTCAACACTTATACTTTTCCATTAGAGACA
CAATACAAGGATAAAAATTTTGCAAAACAAGTTTATGAGAAAGTTGTTCAACGAACATTG
AGCAGTGGAACAACAACTGCTGCATATTTCGCAACTATTCATAAAGACTCGTGTAAAATT
CTTGCTGATGTTGCTAATGAAAAAGGACAACGTGCATTTATTGGAAAAGTTGCTATGAAT
CAATACAGTCCTGACACTTATGTAGAAGAACTTGAACAATCATTAACTGATAATGAAGAA
CTCATAAATTATATAAAAGACCTCAAATCAGATTTGATAACTCCAATTATCACACCAAGA
TTTGCAATTTCATGCAGCATGGAAATGATGAAAAAACTGGCAGAACTTGCTCGAATTCAT
AATTTGCCAATTCAATCACACATTTCTGAAAATGTTGATGAGATTAAATTCACGCTCGAG
CTTTTTTCTGGTCATTCGAACTATGCTGAAGTGTACGACACTGCTGGACTTTTGACAAAT
CGAACTATCATGGCGCATGCTGTTCATCTTGAAAATGAAGAAATTGAATTGTTTGCAAAG
AGAGGCACATCAGTTTCACATTGTCCTAATTCAAATACTAATTTAAGGTCTGGACTGTGT
GATGTGAAAAGATTGATTGCTGGTGGTGTAAAAGTTGGTTTAGGTTCAGATATTTCTGGT
GGAAATCGAATTTCAATTCTTGATGCAATGCGTGCTGCTTTAGATGTCTCTCATCATTTG
AATTTTATGAAAAAGCAAAATGTTCTCGGCACGGGACAAGTTGTTGAAAATAAAGAGAAA
AATGCAGAATATGAACCATTGGATTACAAGCAAGCTCTCTTTTTAGCTACTTTGGGTGGA
GCTCAAGCACTTTCGGTTTCTGATGTTACTGGCAATTTTCAAATAGGAAAGTCTTTTGAT
GCACTCTTGATAGACACATATGAAGGAACTGTAGATGAATTCGATCTTCCAAAAGTGCTA
ACAGAGAATTTATCTGAACATGATAAATTTGAAAAACTTTTACAAAAATTTGTCTACACA
GGTGATGATAGAAATATTATTGAAGTTTTTGTAAAAGGACGACAAGTAAAGACTAAGTAA
>g230.t1 Gene=g230 Length=479
MLSFILNRQYSEIRDDMDHHVIYFGNIFHSISFNESQEILDGFIAVEDGKIIAIGNKTEF
EAWNYANKDTFKKINLTDTQFLIPGFVDSHIHAPQVPNIGLGLDKPLLDWLNTYTFPLET
QYKDKNFAKQVYEKVVQRTLSSGTTTAAYFATIHKDSCKILADVANEKGQRAFIGKVAMN
QYSPDTYVEELEQSLTDNEELINYIKDLKSDLITPIITPRFAISCSMEMMKKLAELARIH
NLPIQSHISENVDEIKFTLELFSGHSNYAEVYDTAGLLTNRTIMAHAVHLENEEIELFAK
RGTSVSHCPNSNTNLRSGLCDVKRLIAGGVKVGLGSDISGGNRISILDAMRAALDVSHHL
NFMKKQNVLGTGQVVENKEKNAEYEPLDYKQALFLATLGGAQALSVSDVTGNFQIGKSFD
ALLIDTYEGTVDEFDLPKVLTENLSEHDKFEKLLQKFVYTGDDRNIIEVFVKGRQVKTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g230.t1 | Gene3D | G3DSA:2.30.40.10 | Urease | 24 | 474 | 0e+00 |
| 7 | g230.t1 | Gene3D | G3DSA:3.20.20.140 | - | 85 | 411 | 0e+00 |
| 2 | g230.t1 | PANTHER | PTHR11271:SF6 | GUANINE DEAMINASE | 19 | 478 | 0e+00 |
| 3 | g230.t1 | PANTHER | PTHR11271 | GUANINE DEAMINASE | 19 | 478 | 0e+00 |
| 1 | g230.t1 | Pfam | PF01979 | Amidohydrolase family | 81 | 476 | 0e+00 |
| 5 | g230.t1 | SUPERFAMILY | SSF51338 | Composite domain of metallo-dependent hydrolases | 23 | 94 | 1e-07 |
| 4 | g230.t1 | SUPERFAMILY | SSF51556 | Metallo-dependent hydrolases | 84 | 412 | 0e+00 |
| 8 | g230.t1 | TIGRFAM | TIGR02967 | guan_deamin: guanine deaminase | 40 | 473 | 0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008892 | guanine deaminase activity | MF |
| GO:0006147 | guanine catabolic process | BP |
| GO:0016787 | hydrolase activity | MF |
| GO:0008270 | zinc ion binding | MF |
| GO:0016810 | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | MF |
KEGG
Orthology
- This transcript has been annotated with K01487 (guanine deaminase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
