General information for g236.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Carbonic anhydrase 14.
Parent gene
- The parent gene g236 is located on chr_3: 1898680..1907157 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g236 | g236.t1 | TSS | g236.t1 | 1898333 | 1898333 |
| chr_3 | g236 | g236.t1 | isoform | g236.t1 | 1898680 | 1907157 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon1 | 1898680 | 1898710 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS1 | 1898680 | 1898710 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon2 | 1903412 | 1903483 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS2 | 1903412 | 1903483 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon3 | 1905344 | 1905502 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS3 | 1905344 | 1905502 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon4 | 1906382 | 1906499 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS4 | 1906382 | 1906499 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon5 | 1906558 | 1906894 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS5 | 1906558 | 1906894 |
| chr_3 | g236 | g236.t1 | exon | g236.t1.exon6 | 1906957 | 1907157 |
| chr_3 | g236 | g236.t1 | cds | g236.t1.CDS6 | 1906957 | 1907157 |
| chr_3 | g236 | g236.t1 | TTS | g236.t1 | NA | NA |
Sequences
>g236.t1 Gene=g236 Length=918
ATGCATAAAGCTTTAGTGATATTCTTAGCAGCATATTTTACCGTTAATTTAGGAGTCGCT
GACTGGCACTATCCAAATGAATTACCCAATGGTGTACTTTCTGATGATGAAGATTGGGGT
GGAATGTGTAGCAGTGGTAAACGTCAATCTCCTGTAGACTTAGCAAAGGATGCTGCTATC
AAAGGAGAATTTCCACTTTTTGATTTCGAATCGTATAAATCAGTCATGAAAAATCCCTTG
ATTGTTAACAATGGACATTCGATTCAAATAAGTTCAGTGCCTGGTCCAATTTACATTAGT
GGTGGCGGATTAAATTCAGTTTATATTTTTGATCAAATGCATTTTCATTGGTCATCAGAA
CACACAATTGATCATAAGAGATTCGCTCTTGAACTTCACATTGTTCATCATGATAAACGT
TATGACACACTTTCACGTGCTGCACAAGAAAAGAATGGAATTGCTGTAGTTGGAATCTTA
TATAAAATTACGACTATTCCTAATCCATTTATTGAGAAACTTTTGGAAAATTCAAAGAAC
ATTTTCGATGCTGCTGGCAAAAATGTTACCTTCCAAGATAAATTAGTGCTTTATGATTTA
TTGCCAAGAAATAAGCAAAGATACTTTAGATATGAAGGATCATTGACTACTCCATCATGT
GGTGAGGCTGTAATTTGGACTGTCTTTGAAGATGCTATTGGAATTACTATTGAACAACTT
GAAAGATTCAAATCAGTTCATGATGACTCAGGACATGAATTGACCCACAATTATCGTCAT
ATTCAGCCATTAAATAGTCGTGCATTGATTTATGTTGAAGGCGAATACAATCCAAATTCA
GCTAATTCTTTCAAATCATTCTCAGCTTCAATTTTGATCATTTCTGTCATCTTTGCCTTC
ATTGGAATGAAAATTTAA
>g236.t1 Gene=g236 Length=305
MHKALVIFLAAYFTVNLGVADWHYPNELPNGVLSDDEDWGGMCSSGKRQSPVDLAKDAAI
KGEFPLFDFESYKSVMKNPLIVNNGHSIQISSVPGPIYISGGGLNSVYIFDQMHFHWSSE
HTIDHKRFALELHIVHHDKRYDTLSRAAQEKNGIAVVGILYKITTIPNPFIEKLLENSKN
IFDAAGKNVTFQDKLVLYDLLPRNKQRYFRYEGSLTTPSCGEAVIWTVFEDAIGITIEQL
ERFKSVHDDSGHELTHNYRHIQPLNSRALIYVEGEYNPNSANSFKSFSASILIISVIFAF
IGMKI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g236.t1 | CDD | cd00326 | alpha_CA | 46 | 267 | 8.44739E-82 |
| 6 | g236.t1 | Gene3D | G3DSA:3.10.200.10 | Carbonic Anhydrase II | 18 | 283 | 3.1E-72 |
| 2 | g236.t1 | PANTHER | PTHR18952:SF238 | CARBONIC ANHYDRASE 5-RELATED | 11 | 270 | 6.6E-64 |
| 3 | g236.t1 | PANTHER | PTHR18952 | CARBONIC ANHYDRASE | 11 | 270 | 6.6E-64 |
| 1 | g236.t1 | Pfam | PF00194 | Eukaryotic-type carbonic anhydrase | 22 | 269 | 8.2E-70 |
| 9 | g236.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 10 | g236.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 11 | g236.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 13 | g236.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 8 | g236.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 285 | - |
| 12 | g236.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 286 | 303 | - |
| 7 | g236.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 304 | 305 | - |
| 15 | g236.t1 | ProSitePatterns | PS00162 | Alpha-carbonic anhydrases signature. | 119 | 135 | - |
| 17 | g236.t1 | ProSiteProfiles | PS51144 | Alpha-carbonic anhydrases profile. | 20 | 273 | 57.582 |
| 16 | g236.t1 | SMART | SM01057 | Carb_anhydrase_2a | 22 | 271 | 6.5E-88 |
| 4 | g236.t1 | SUPERFAMILY | SSF51069 | Carbonic anhydrase | 31 | 269 | 6.28E-69 |
| 5 | g236.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008270 | zinc ion binding | MF |
| GO:0004089 | carbonate dehydratase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01672 (carbonic anhydrase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed