General information for g2417.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kinase suppressor of Ras 2.
Parent gene
- The parent gene g2417 is located on chr_3: 17861389..17864642 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2417 | g2417.t1 | TSS | g2417.t1 | 17860885 | 17860885 |
| chr_3 | g2417 | g2417.t1 | isoform | g2417.t1 | 17861389 | 17864642 |
| chr_3 | g2417 | g2417.t1 | exon | g2417.t1.exon1 | 17861389 | 17861941 |
| chr_3 | g2417 | g2417.t1 | cds | g2417.t1.CDS1 | 17861389 | 17861941 |
| chr_3 | g2417 | g2417.t1 | exon | g2417.t1.exon2 | 17862000 | 17863553 |
| chr_3 | g2417 | g2417.t1 | cds | g2417.t1.CDS2 | 17862000 | 17863553 |
| chr_3 | g2417 | g2417.t1 | exon | g2417.t1.exon3 | 17863624 | 17864642 |
| chr_3 | g2417 | g2417.t1 | cds | g2417.t1.CDS3 | 17863624 | 17864642 |
| chr_3 | g2417 | g2417.t1 | TTS | g2417.t1 | 17864725 | 17864725 |
Sequences
>g2417.t1 Gene=g2417 Length=3126
ATGGCAGATGAAGGGTTGGAGGTAATTCAGTCGATACGTGAAGTAATCGACATCTCAGCT
GATCATCTTGATCGCTTGAGGACGCAATGTGCGACAAGTGTCTTAACACTACAGGAAACG
CGTAAACTCGAGTCAAAACTTGTTCGAATGTTTTGTGAATTACTTCTAAGTCGACAAAAT
ATGTCTTCATCGGATGTGAGTCTCGATATGTCATGTTCATCGTCATCGACAACAAACACT
TCCACACCAAACAATGATGATCTGCATCAGTGGTTAAGAGTTGTTGGCCTAAGCCAGCAA
ACAATAAATGCTGTGATCCAGAATGTAAATTCATTAGAAACATTGCTGAAAATGAATGAC
GATGATATTTGTGCAATATTAGTAACGAGTAATCAAAATGACGAAGCACGTCGCTTAGTC
TGTGCCATAAACAATCTGAAGCGTTATCAGCAATGCTTGAAGGCGGGAACAGAACCATCG
GATATGTTTTGGGACTCATGGGATAGACGCAATAACATTCGTTTGGGCACATCACCGAAA
ATATCAAATAATCGTTCATGTCGACCATTGGGTGATACAGTGATTATTAATAGCGAGCAA
CAACAGGATGATTTAATTCAATCACCAGTTGAGTTATCACCACCTGAGACACAAATTTCC
ACTTCAAGCCTAATGACAACTGGAACACTAACATCATCGTCCGATAAACTTCTCAATATG
ATCTCACCAGAGGAATGCTCTGTTAATTTATCACCGAGTCCACCACAAAGTCCACCTGTA
CATAATAATCATAACAACAGCACATCACAATCGTCATTATCATTAGGAAGCAATAAGAGG
CTGGCCCAAAATAATCAGAAAATCCATCATAACAATATCAATAATAACATTTTGAACTTT
AACAATCTGAAGCAGGATAATAATGGTATCGTGTGCAGTAGCAGTATTTTAACTACTTCA
ATTACTTCAAATAATATTAATAATAATAATAATTTAAATAATAATATTAATAATAATTAT
CATGATAATACTGAGTTTCCAACTTCAAACACAACAAACTCTTTGGACGCGTGCGAGGAC
TCAAATATCGATGGCATGAGCAAATCAAAATCAAATGAGTCCCAATTATCAGTGAATGGT
GGAGTTGGTGGAAATAATAAGCAATGGCAACAGCAAAATGGCTCGGCAATGAGCAGTTCT
GAAATGTCGAGCAATGAAATGGTGCCAGTTCCAGCACCAAGAACAAAATATCCAATTACG
ACAACTTCAACAGACTTTAACAATCATCAGCAACAAAATAATGAAAATAGTAATAGTTTC
AATGAAATGAGCACTTCTGGCAGCATTAGCAGTCATAGTGTGGTAACACCTATTATCGTT
AGTGACACATCAGCACCACCTCGTTCGCCTTGCACGCCTGTCAGAGGAATGGGTCACATG
ATACAGCATCGTTTCTCAAAGAAATTCAAATTTACTAGTAGTACCTGTGATCTGTGTAAT
AAGCAAATGTTTTTTGGCTTCAAGTGCACCGAATGTAAATATCGCTGCCATAAAGATTGC
AAATCAAATGTTCCGCCATCGTGTGGTCTACCGAAAGAATTTGTCGATGAATTTAAAAAG
ACCTTATATTCTGATGGTCTGATGCCAAATGCATCGCCAAATTTACCACGCGCATTAATG
CCTATCAAACGAGAAACTCGTCATCGTCCGCCAATGCATTCAATTCCATTTCATGACAGC
AGCTCAGCAGGTTCTAGCTGCAACAGTTCATCACCTTCAAGTCCTGCACTTCTCACTATA
CCACCACAAACGCCAGCCAATAAATCGCAATTTAATTTTCCTGAAGTATCTCCATCAACC
GTCTCATCACTTTCTAATCAAAATTCGCATCACAATAGCAGCAATTTTTCTTCTTATCCA
AATACAATCATTGAAGATGCTGAGCTCGAACAACAACAATCTGGGCAACATTCCAAAACG
ATTGGTAACAATTCTGCTAGTGATAATAACACGGGAATGTATTTAAGAAACAATTATCAG
CATTCATTTGCATCACAAAATAACAATATTGATAATCAACTAAATGGGAGTATCATTGAC
AGTAGCGGTACTCATCAAAGCATAGAGAGTGATAAAACAATGAGTACAGAGTCAGATCGC
ATGACACCGAGACTTGAATCAGTAGAAGAGCGTGATAGTATTTGGCCGAGACAAAATAGT
CTTTCATTGAAAGAATGGGATATACCATATGATGATTTGAATTTGGGTACGATGATTGGA
AAAGGTAGATTTGGTACTGTATATAGAGGAAATTGGCATGGAGATGTTGCTGTTAAAATT
CTCAAGGAAGATTTTCTAGATGATGAGCATGCAATTGAAGCTTTCAAATTGGAAGTAGCA
ATATTTAAAAATACGCGTCATGAGAATTTAGTGCTTTTTATGGGTGCTTGCATGAAACCA
CCTCGATTAGCAATTGTTACATCGATGTGCAAAGGAAATACTCTCTATACACATGTGCAT
TTGCGACGTGACAAATTCAATTTAAATAAAACCACGCTTATTGCTCAACAGATATCACAA
GGAATGGGCTATTTACATCGACGTGGTATTGTTCATAAAGATCTCAAATCTAAAAATATT
TTCCTCGAGAACGAGAAAGTGATTATTACTGATTTTGGACTTTTTAGTGTAACAAAATTA
GTGTACGATAGTAATTTAGGACTTGGTATTCCCTACGGTTGGCTTTGCTATATGGCACCA
GAATTGATTCGCAACTTAAAGCCCTATCGACCTACTAATGAGGATTTGCAATTTACAAAA
GCTTCAGATACTTATGCTTTTGGAACTATTTGGTATGAATTACTATGCGGTGAATTTCCC
TTTAAATCGCAACCGCCAGAATCGGTAATTTGGCAAGTCGGTCGCGGAATGAAACAATCA
TTATCAAATTTACAAGCATCACGTGATGTCAAAGATATTTTAATGATGTGTTGGACATTC
AATGCTGACGATCGTCCAGACTTTAACAAACTTTTTACAACACTCGAGCGCTTGCCAAAA
AAGCGTCTTGCTCGTTCACCATCACATCCAATTCAATTGTCTCGATCAGCAGAATCGGTT
TTCTAG
>g2417.t1 Gene=g2417 Length=1041
MADEGLEVIQSIREVIDISADHLDRLRTQCATSVLTLQETRKLESKLVRMFCELLLSRQN
MSSSDVSLDMSCSSSSTTNTSTPNNDDLHQWLRVVGLSQQTINAVIQNVNSLETLLKMND
DDICAILVTSNQNDEARRLVCAINNLKRYQQCLKAGTEPSDMFWDSWDRRNNIRLGTSPK
ISNNRSCRPLGDTVIINSEQQQDDLIQSPVELSPPETQISTSSLMTTGTLTSSSDKLLNM
ISPEECSVNLSPSPPQSPPVHNNHNNSTSQSSLSLGSNKRLAQNNQKIHHNNINNNILNF
NNLKQDNNGIVCSSSILTTSITSNNINNNNNLNNNINNNYHDNTEFPTSNTTNSLDACED
SNIDGMSKSKSNESQLSVNGGVGGNNKQWQQQNGSAMSSSEMSSNEMVPVPAPRTKYPIT
TTSTDFNNHQQQNNENSNSFNEMSTSGSISSHSVVTPIIVSDTSAPPRSPCTPVRGMGHM
IQHRFSKKFKFTSSTCDLCNKQMFFGFKCTECKYRCHKDCKSNVPPSCGLPKEFVDEFKK
TLYSDGLMPNASPNLPRALMPIKRETRHRPPMHSIPFHDSSSAGSSCNSSSPSSPALLTI
PPQTPANKSQFNFPEVSPSTVSSLSNQNSHHNSSNFSSYPNTIIEDAELEQQQSGQHSKT
IGNNSASDNNTGMYLRNNYQHSFASQNNNIDNQLNGSIIDSSGTHQSIESDKTMSTESDR
MTPRLESVEERDSIWPRQNSLSLKEWDIPYDDLNLGTMIGKGRFGTVYRGNWHGDVAVKI
LKEDFLDDEHAIEAFKLEVAIFKNTRHENLVLFMGACMKPPRLAIVTSMCKGNTLYTHVH
LRRDKFNLNKTTLIAQQISQGMGYLHRRGIVHKDLKSKNIFLENEKVIITDFGLFSVTKL
VYDSNLGLGIPYGWLCYMAPELIRNLKPYRPTNEDLQFTKASDTYAFGTIWYELLCGEFP
FKSQPPESVIWQVGRGMKQSLSNLQASRDVKDILMMCWTFNADDRPDFNKLFTTLERLPK
KRLARSPSHPIQLSRSAESVF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g2417.t1 | CDD | cd00029 | C1 | 483 | 528 | 1.33557E-13 |
| 12 | g2417.t1 | CDD | cd14063 | PK_KSR | 752 | 1021 | 1.56654E-159 |
| 11 | g2417.t1 | Gene3D | G3DSA:1.10.150.50 | Transcription Factor | 74 | 158 | 2.4E-5 |
| 9 | g2417.t1 | Gene3D | G3DSA:3.30.60.20 | - | 480 | 529 | 1.5E-17 |
| 8 | g2417.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 740 | 831 | 6.4E-34 |
| 10 | g2417.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 832 | 1019 | 8.1E-44 |
| 20 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 246 | 277 | - |
| 22 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 260 | 277 | - |
| 24 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 381 | 405 | - |
| 21 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 613 | 634 | - |
| 19 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 702 | 731 | - |
| 23 | g2417.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 712 | 731 | - |
| 4 | g2417.t1 | PANTHER | PTHR23257 | SERINE-THREONINE PROTEIN KINASE | 456 | 1039 | 1.6E-128 |
| 5 | g2417.t1 | PANTHER | PTHR23257:SF716 | KINASE SUPPRESSOR OF RAS 1 | 456 | 1039 | 1.6E-128 |
| 1 | g2417.t1 | Pfam | PF13543 | SAM like domain present in kinase suppressor RAS 1 | 10 | 147 | 7.2E-31 |
| 3 | g2417.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 483 | 529 | 1.9E-8 |
| 2 | g2417.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 753 | 1014 | 6.1E-56 |
| 15 | g2417.t1 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 483 | 528 | - |
| 16 | g2417.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 759 | 779 | - |
| 14 | g2417.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 870 | 882 | - |
| 25 | g2417.t1 | ProSiteProfiles | PS50105 | SAM domain profile. | 83 | 149 | 8.918 |
| 26 | g2417.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 482 | 528 | 12.065 |
| 27 | g2417.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 753 | 1018 | 37.121 |
| 18 | g2417.t1 | SMART | SM00109 | c1_12 | 483 | 528 | 9.8E-12 |
| 17 | g2417.t1 | SMART | SM00220 | serkin_6 | 753 | 1018 | 1.1E-41 |
| 6 | g2417.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 468 | 531 | 1.5E-14 |
| 7 | g2417.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 727 | 1014 | 1.27E-63 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0005515 | protein binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K18529 (kinase suppressor of Ras 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
