General information for g2421.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aminopeptidase N.
Parent gene
- The parent gene g2421 is located on chr_3: 17881631..17884779 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2421 | g2421.t1 | TTS | g2421.t1 | 17878481 | 17878481 |
| chr_3 | g2421 | g2421.t1 | isoform | g2421.t1 | 17878582 | 17882314 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon1 | 17878582 | 17878807 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS1 | 17878582 | 17878807 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon2 | 17878861 | 17879169 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS2 | 17878861 | 17879169 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon3 | 17879227 | 17879420 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS3 | 17879227 | 17879420 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon4 | 17879532 | 17880125 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS4 | 17879532 | 17880125 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon5 | 17880186 | 17880372 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS5 | 17880186 | 17880372 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon6 | 17880428 | 17880724 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS6 | 17880428 | 17880724 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon7 | 17880786 | 17880856 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS7 | 17880786 | 17880856 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon8 | 17880911 | 17880961 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS8 | 17880911 | 17880961 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon9 | 17881137 | 17881166 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS9 | 17881137 | 17881166 |
| chr_3 | g2421 | g2421.t1 | exon | g2421.t1.exon10 | 17881226 | 17882314 |
| chr_3 | g2421 | g2421.t1 | cds | g2421.t1.CDS10 | 17881226 | 17882314 |
| chr_3 | g2421 | g2421.t1 | TSS | g2421.t1 | NA | NA |
Sequences
>g2421.t1 Gene=g2421 Length=3048
ATGCGCGATACATTTACAATAGAGAATTCAAGGCATCCGGGAGATTATGATACAAAGAAG
CACTTCCTTAACTTAAGTCGCTTGACGTTCTTTTTGCTCCTGGCTATGTTCATTTGCAGT
TTAGTGGCTGTTGCTCTTCTAGTCTACAATTTTGCTGTATGTCCTCAAGACGATGATGTC
AAAGTATGCTCAAAACATTATCATATCAACGAAAATTCATCACCATCAACAACATCAATT
GCATCTATAACTGATATTGGTGAAACAACAGAACCACCAATTATAAAAGATGTTCGATTA
CCGAGATCTATTAAACCTTTATCATATGACATTGTGATTGTTCCAAATTTAAATGGCGAA
AATTTCACATTCGAGGGCGATGTCGCTATAAAAATTCACGTGGATGAGTCATGCAACAAT
ATCACTTTACATTCATGGACTTTGAAAATTCGACGAGATTTTACGAAAATATTTATTTTT
GACATTGATGGAAATGTAACCGCTGATCAAATTGATATTAAAAATCAATTTTTTATTGAT
GAAAAACAATTTCTTGTGCTTGAGACTAAACAAGAACTGGAAAAGGGAAAAAATTATTTA
TTAAAGATCAAATATATTGGACAAATAACAGATAATTTGCAGGGTTTTTATAAATCATCA
TATACAGTCAATGGAGAGACAAAGTGGTTGGTATCAACACAATTTCAATCAACAGATGCT
CGCAGATGCTTTCCGTGTTGGGACGAGCCAGAATTTAAAGCTACTTTTAAAATTACTTTA
GGCAGACCAAAAACAATGAACTCATTGTCAAATATGCCCCTTGAGAGAATAGATACACAA
AATGATCGTGTTTCAACACCTCTTCCTGATTATGTCTATGACATTTATTCCGAATCTGTC
AAAATGTCAACTTATCTTGTTGCATTTGTTGTCAGTGATTTTGTGAATAATTCTGATAGT
TCAGTTTCAGTTTGGGCTCGTTCTGATACAATTCAATCAACTAACTATGCACTTTCAATT
GCTCCAAAAGTTATAAAATTCTTAGAAGATTTTTTTGATATCAAATATCCTTTACCAAAA
GTGGACATGATTGCAATTCCCGATTTCCAATTTGGAGCTATGGAAAATTTTGGGTTGATC
ACCTTTAGGGAAACTGCTATGCTTTATGATGAAGGAGTGTCAGCAATAGGAAAGAAGATG
ACAGTTGCTTTAATTTGTGCTCATGAAATAGCTCATCAATGGTTTGGAAATTTAGTTTCT
CCACACTGGTGGTCAGATCTTTGGTTAAATGAAGTTGAGTATTTTTTTACTATTAAAATT
AAATTTGGTATTAATTCAAAATTTCATATTATAGGCTTTGCAACATTCATGGAAGATGTA
ATCGTTGACCATATTGAGCCAGCTTGGAACAGTAAAGATCTTTTTGTAGTCAATGAACTT
CACCAAGTTTTTAGACTCGATGCTCTTTTATCATCACATAAAGTTTCAGTTGAAGTTCAA
AATCCAGATCAAATTAGTGAAATTTTCGATTCAATATCTTATTCAAAAGGAGCAGCACTT
ATTCGCTCAATGGAATATTTTCTCACAACTGAAGTCTTTCGACGCGGCTTATCGAATTAT
CTTAAGGAGTTTTCATATAGAACAGCGACACAGAATGATTTATGGAATGCATTGACAAAA
GAAGCCCATCGTTCAAATGTCCTCAATGAAAATATGTCAGTTAAGGAAATAATGGATGGT
TGGACCTTACAAACTGGTCTTCCCTATTTAACAGTTACTAGAAATTATGAAAATAACGAA
ATAACACTCGAACAAAAAAGATTTATTTTAATTGAAGCAAATTCTACAGAAGCCGCAACA
TCGAATGATAACGATGATAAAGATCCACTCTGGTGGATTCCAATATCATATACGACACAA
GCTGAATTAGATTTTGATAAAACTCATCCATCGCAATGGATGCAAGCAACTCGAAAAATT
ACAATAGAAGAACAACTCGATGAGAATCAATGGATAATTTTTAATCCACAAGTTGCTCAC
TATTATCGTGTCAACTACGACTCACAGAACTGGAAACTCATCACAGATCATCTCAATGAT
CCTCAACGTTACAAACAAATTGCCAATGCAAATCGTGCTCAATTGATTGATGACTCAATG
AATTTGGCACGTGCTGACATTCTCGATTATACTGTCGCTATGGATATGACAAAGTACTTA
AAGCACGAAAAAGATTTTGTACCGTGGAAAACTGCAATCAACAATTTGCTATACGTTGAC
TCAATGCTCATCCGCACGCCTGATTATAATTTAATGAAGAAGTACTTTAGATCGAGAATA
GAAAACATTTATGAACAGCTTGGATTTGAAGATCGTGGCGATATTTTGACGATGCTTAGC
AGACATGAGATTTTAAAAGCAGCTTGTCACTTGGGAATTCCTGATTGTATTGCAAATTCT
ATTAAAGAATTTCATAAGTGGAAAATGGAAGCTAATCCTGACATTAATAATCCCATTTCA
CCAAATCTTAAAATTGTCGTTTATTGCACTGCAATAAAACATGGAGATGAAGAAGAATGG
GATTTTGCATGGGATCGTTATTTGAACACAACAATGTCAAGTGAAAAGGAATTATTGATG
CAAGCACTTGGTTGCACAAGACAACCATGGTTATTGAGTAGATATCTTGAAAGAACATTA
GAAAATGACGGCATCAGAAAACAAGACACATTCAGAGTTTTTTCAGCTGTTTCAAACAAT
GTTTGGGGTCAATCAATTGCATTTAATTTCATTAAAAATAATTGGAAGAGAATTCGAAAT
CATTTCGGAGTTGGTTCACCAATGAGTACATTGAATTCTCTCATAAAATTCGTCACGAAA
GGAATGACAGATAAAAACCAACTCGATGATTTAAAGAGATTTGATGATAAAGAATTGAAA
CAAGGGCGCACATTAAAACAAGCGATTGAACAAGCTGAGGCGAATATTTTATGGTTAAAC
AAAAATTATAAGAAAATTGTCGATTGGCTAAAAGAGAATTCTGATTAA
>g2421.t1 Gene=g2421 Length=1015
MRDTFTIENSRHPGDYDTKKHFLNLSRLTFFLLLAMFICSLVAVALLVYNFAVCPQDDDV
KVCSKHYHINENSSPSTTSIASITDIGETTEPPIIKDVRLPRSIKPLSYDIVIVPNLNGE
NFTFEGDVAIKIHVDESCNNITLHSWTLKIRRDFTKIFIFDIDGNVTADQIDIKNQFFID
EKQFLVLETKQELEKGKNYLLKIKYIGQITDNLQGFYKSSYTVNGETKWLVSTQFQSTDA
RRCFPCWDEPEFKATFKITLGRPKTMNSLSNMPLERIDTQNDRVSTPLPDYVYDIYSESV
KMSTYLVAFVVSDFVNNSDSSVSVWARSDTIQSTNYALSIAPKVIKFLEDFFDIKYPLPK
VDMIAIPDFQFGAMENFGLITFRETAMLYDEGVSAIGKKMTVALICAHEIAHQWFGNLVS
PHWWSDLWLNEVEYFFTIKIKFGINSKFHIIGFATFMEDVIVDHIEPAWNSKDLFVVNEL
HQVFRLDALLSSHKVSVEVQNPDQISEIFDSISYSKGAALIRSMEYFLTTEVFRRGLSNY
LKEFSYRTATQNDLWNALTKEAHRSNVLNENMSVKEIMDGWTLQTGLPYLTVTRNYENNE
ITLEQKRFILIEANSTEAATSNDNDDKDPLWWIPISYTTQAELDFDKTHPSQWMQATRKI
TIEEQLDENQWIIFNPQVAHYYRVNYDSQNWKLITDHLNDPQRYKQIANANRAQLIDDSM
NLARADILDYTVAMDMTKYLKHEKDFVPWKTAINNLLYVDSMLIRTPDYNLMKKYFRSRI
ENIYEQLGFEDRGDILTMLSRHEILKAACHLGIPDCIANSIKEFHKWKMEANPDINNPIS
PNLKIVVYCTAIKHGDEEEWDFAWDRYLNTTMSSEKELLMQALGCTRQPWLLSRYLERTL
ENDGIRKQDTFRVFSAVSNNVWGQSIAFNFIKNNWKRIRNHFGVGSPMSTLNSLIKFVTK
GMTDKNQLDDLKRFDDKELKQGRTLKQAIEQAEANILWLNKNYKKIVDWLKENSD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g2421.t1 | CDD | cd09601 | M1_APN-Q_like | 107 | 583 | 0.0 |
| 14 | g2421.t1 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 93 | 313 | 8.0E-72 |
| 15 | g2421.t1 | Gene3D | G3DSA:1.10.390.60 | - | 314 | 438 | 2.2E-51 |
| 16 | g2421.t1 | Gene3D | G3DSA:1.10.390.60 | - | 451 | 586 | 6.7E-45 |
| 13 | g2421.t1 | Gene3D | G3DSA:2.60.40.1910 | - | 587 | 686 | 4.2E-25 |
| 17 | g2421.t1 | Gene3D | G3DSA:1.25.50.20 | - | 687 | 1015 | 3.8E-112 |
| 5 | g2421.t1 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 89 | 1013 | 0.0 |
| 6 | g2421.t1 | PANTHER | PTHR11533:SF246 | AMINOPEPTIDASE | 89 | 1013 | 0.0 |
| 9 | g2421.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 241 | 256 | 1.6E-17 |
| 8 | g2421.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 369 | 379 | 1.6E-17 |
| 7 | g2421.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 405 | 420 | 1.6E-17 |
| 10 | g2421.t1 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 424 | 436 | 1.6E-17 |
| 4 | g2421.t1 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 105 | 306 | 8.3E-49 |
| 2 | g2421.t1 | Pfam | PF01433 | Peptidase family M1 domain | 336 | 431 | 3.1E-38 |
| 3 | g2421.t1 | Pfam | PF01433 | Peptidase family M1 domain | 452 | 581 | 7.2E-32 |
| 1 | g2421.t1 | Pfam | PF11838 | ERAP1-like C-terminal domain | 671 | 993 | 4.4E-85 |
| 18 | g2421.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 27 | - |
| 20 | g2421.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 28 | 52 | - |
| 19 | g2421.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 53 | 1015 | - |
| 12 | g2421.t1 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 97 | 313 | 2.75E-47 |
| 11 | g2421.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 321 | 592 | 1.32E-76 |
| 22 | g2421.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11140 (aminopeptidase N)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
