General information for g2428.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as G protein-coupled receptor kinase 2.
Parent gene
- The parent gene g2428 is located on chr_3: 17928396..17931440 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2428 | g2428.t1 | isoform | g2428.t1 | 17925626 | 17930434 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon1 | 17925626 | 17925721 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS1 | 17925626 | 17925721 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon2 | 17925792 | 17926055 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS2 | 17925792 | 17926055 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon3 | 17926126 | 17926736 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS3 | 17926126 | 17926736 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon4 | 17926954 | 17927143 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS4 | 17926954 | 17927143 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon5 | 17927408 | 17927513 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS5 | 17927408 | 17927513 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon6 | 17928212 | 17928311 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS6 | 17928212 | 17928311 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon7 | 17928389 | 17928660 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS7 | 17928389 | 17928660 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon8 | 17928853 | 17928967 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS8 | 17928853 | 17928967 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon9 | 17929631 | 17929726 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS9 | 17929631 | 17929726 |
| chr_3 | g2428 | g2428.t1 | exon | g2428.t1.exon10 | 17930383 | 17930434 |
| chr_3 | g2428 | g2428.t1 | cds | g2428.t1.CDS10 | 17930383 | 17930434 |
| chr_3 | g2428 | g2428.t1 | TSS | g2428.t1 | 17931433 | 17931433 |
| chr_3 | g2428 | g2428.t1 | TTS | g2428.t1 | NA | NA |
Sequences
>g2428.t1 Gene=g2428 Length=1902
ATGGAGTTAGAGAACATTGTGGCCAATACGGTCTACCTCAAAGCCAGAGAGGGTGGCTCT
GATAGTAACAAAGGAAAGAGCAAGAAATGGCGCAAAATTTTACAATTTCCACACATATCT
CAATGTATAGATCTCAAAAATAAAATAGATGTAAGCTATGATTATGTCGTAGACAAACAA
CCAATTGGAAGAGTATTGTTTCGGCAGTTTTGTCAAGTCAAACAATTTTACAAATATGTA
AAATTCTTAGAAGAAGTATCAAGGTATGAGTTGGAGTCTGATGAAAATCGACAGGCAATT
GCCATTAGAATAGGGCGACAATTTTTGGGTATTGAAGAGGATGAAATTAAAGCGATAGAT
CAGGAGAATGAAAAAACTGACTCGAACAATATCAATCCAAATAATGTTTCATCACCAAAA
AACAACTCATCACAGCTCGATAAAGCTGATGGCCAATCGGAACAGAATGAAATATCTCCC
GTCACTGACATTCTAAATGATGAACTCATTTCAGACTGTAAGCAAAAACTTCACATCGCT
TCAAAAGAGTTGTTTGAACCGTGCTTTGTAGCAGTTAAAACGTATTTGGCAGGAGAGCCA
TTTAGAGAATTTGAGACTTCAATGTATTTCCATAGATATCTTCAATGGAAATGGCTCGAA
GCTCAGCCGGTTACATATAAAACTTTTCGCATGTATCGAGTCCTGGGCAAGGGTGGTTTT
GGTGAAGTGTGTGCTTGCCAAGTACGAGCAACTGGAAAAATGTATGCCTGCAAGAAACTC
GAGAAAAAGCGAATAAAGAAGCGAAAGGGTGAATCAATGGTGCTTATTGAGAAACAGATA
TTGCAAAAGATCAACTCACGATTTGTCGTAAATTTGGCATATGCTTATGAGACTAAAGAT
GCTTTATGTCTTGTTCTTACAATCATGAACGGTGGAGATTTAAAATTTCATATCTATAAT
ATGGGTGGTGAGCCAGGTTTTGAGTTACCTAGAGCGCGTTTCTATTCAGCTGAGGTTGTG
TGTGGCCTAGAGCATCTGCATCAGCAGGGAATTGTTTATAGAGACTGCAAACCAGAAAAT
ATTTTACTGGATGACCATGGTCATGTGAGAATTTCAGATTTGGGATTAGCTGTTGAAATA
CCAGAAGGTGAAATGGTGCGTGGTCGTGTTGGAACTGTTGGTTATATGGCACCAGAAGTC
ATTGATAATGAAAAATATTCATTTTCACCGGACTGGTTTAGTTTTGGTTGTCTCATATAC
GAAATGATTGAAGGTCAGGCGCCATTTAGAGCTCGAAAAGAGAAAGTGAAACGAGAAGAA
GTTGATAGACGTGTGAAAGAAGATGCCGAAAAATATTCATCAAAATTTAGTGAAGAAGCC
AAATCGCTGTGTCAACAATTGTTAATAAAATCACCGAAGAATCGATTGGGTTGTAAAAAT
GGTCGCTATGGAGCACGTGAAGTTAAGTTATCATCATTCTTTAGCGCCATCAATTGGAAA
CGACTTGAAGCAGGCATGGTGGAGCCCCCATTTATTCCAGACCCACATGCTGTTTACGCA
AAAGATGTATTAGACATTGAACAATTTTCTACGGTGAAAGGTGTTAATTTAGATGCTACT
GATGAGAATTTCTATTCTAAATTCAATACCGGATCAGTGTCGATACCATGGCAAAATGAA
ATGATTGAAACTGATTGTGTGCGAGAACTGAATATCTTTGGTCCAGACGATAGTCCGACT
CCCGATTTGATATTGAATGCACCCATTATTGAAGAAAAACAAGGATGCTTTCCATTTAGA
AGAAAGAAAAAGCAACTTCCTCGAACAAAACCAATTCCCGTACCCGAACATCTTTTGACA
CCTAGTCAAGCAGAAACAATAAGCGCAACAAATGAAAGCTGA
>g2428.t1 Gene=g2428 Length=633
MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCIDLKNKIDVSYDYVVDKQ
PIGRVLFRQFCQVKQFYKYVKFLEEVSRYELESDENRQAIAIRIGRQFLGIEEDEIKAID
QENEKTDSNNINPNNVSSPKNNSSQLDKADGQSEQNEISPVTDILNDELISDCKQKLHIA
SKELFEPCFVAVKTYLAGEPFREFETSMYFHRYLQWKWLEAQPVTYKTFRMYRVLGKGGF
GEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSRFVVNLAYAYETKD
ALCLVLTIMNGGDLKFHIYNMGGEPGFELPRARFYSAEVVCGLEHLHQQGIVYRDCKPEN
ILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYSFSPDWFSFGCLIY
EMIEGQAPFRARKEKVKREEVDRRVKEDAEKYSSKFSEEAKSLCQQLLIKSPKNRLGCKN
GRYGAREVKLSSFFSAINWKRLEAGMVEPPFIPDPHAVYAKDVLDIEQFSTVKGVNLDAT
DENFYSKFNTGSVSIPWQNEMIETDCVRELNIFGPDDSPTPDLILNAPIIEEKQGCFPFR
RKKKQLPRTKPIPVPEHLLTPSQAETISATNES
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g2428.t1 | CDD | cd05605 | STKc_GRK4_like | 228 | 515 | 0.0 |
| 12 | g2428.t1 | Coils | Coil | Coil | 631 | 633 | - |
| 18 | g2428.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 121 | 153 | - |
| 19 | g2428.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 125 | 153 | - |
| 17 | g2428.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 610 | 633 | - |
| 3 | g2428.t1 | PANTHER | PTHR24355:SF28 | G PROTEIN-COUPLED RECEPTOR KINASE 2 | 1 | 625 | 0.0 |
| 4 | g2428.t1 | PANTHER | PTHR24355 | G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE | 1 | 625 | 0.0 |
| 8 | g2428.t1 | PRINTS | PR00717 | GPCR kinase signature | 210 | 222 | 3.8E-37 |
| 6 | g2428.t1 | PRINTS | PR00717 | GPCR kinase signature | 268 | 286 | 3.8E-37 |
| 5 | g2428.t1 | PRINTS | PR00717 | GPCR kinase signature | 509 | 526 | 3.8E-37 |
| 7 | g2428.t1 | PRINTS | PR00717 | GPCR kinase signature | 532 | 545 | 3.8E-37 |
| 9 | g2428.t1 | PRINTS | PR00717 | GPCR kinase signature | 547 | 564 | 3.8E-37 |
| 1 | g2428.t1 | Pfam | PF00615 | Regulator of G protein signaling domain | 53 | 213 | 6.0E-10 |
| 2 | g2428.t1 | Pfam | PF00069 | Protein kinase domain | 231 | 480 | 2.0E-64 |
| 16 | g2428.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 235 | 267 | - |
| 20 | g2428.t1 | ProSiteProfiles | PS50132 | RGS domain profile. | 59 | 214 | 19.536 |
| 22 | g2428.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 229 | 494 | 41.587 |
| 21 | g2428.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 495 | 560 | 10.524 |
| 14 | g2428.t1 | SMART | SM00315 | RGS_3 | 52 | 214 | 2.1E-14 |
| 15 | g2428.t1 | SMART | SM00133 | pkinase_C_6 | 495 | 574 | 3.6E-11 |
| 11 | g2428.t1 | SUPERFAMILY | SSF48097 | Regulator of G-protein signaling, RGS | 31 | 224 | 1.6E-28 |
| 10 | g2428.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 226 | 574 | 2.16E-79 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004703 | G protein-coupled receptor kinase activity | MF |
| GO:0007165 | signal transduction | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08291 (G protein-coupled receptor kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
