General information for g2428.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as G protein-coupled receptor kinase 2.
Parent gene
- The parent gene g2428 is located on chr_3: 17928396..17931440 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2428 | g2428.t2 | isoform | g2428.t2 | 17925626 | 17926427 |
| chr_3 | g2428 | g2428.t2 | exon | g2428.t2.exon1 | 17925626 | 17925721 |
| chr_3 | g2428 | g2428.t2 | cds | g2428.t2.CDS1 | 17925626 | 17925721 |
| chr_3 | g2428 | g2428.t2 | exon | g2428.t2.exon2 | 17925792 | 17926055 |
| chr_3 | g2428 | g2428.t2 | cds | g2428.t2.CDS2 | 17925792 | 17926055 |
| chr_3 | g2428 | g2428.t2 | exon | g2428.t2.exon3 | 17926126 | 17926427 |
| chr_3 | g2428 | g2428.t2 | cds | g2428.t2.CDS3 | 17926126 | 17926404 |
| chr_3 | g2428 | g2428.t2 | TSS | g2428.t2 | NA | NA |
| chr_3 | g2428 | g2428.t2 | TTS | g2428.t2 | NA | NA |
Sequences
>g2428.t2 Gene=g2428 Length=662
GTTTTGGTTGTCTCATATACGAAATGATTGAAGGTCAGGCGCCATTTAGAGCTCGAAAAG
AGAAAGTGAAACGAGAAGAAGTTGATAGACGTGTGAAAGAAGATGCCGAAAAATATTCAT
CAAAATTTAGTGAAGAAGCCAAATCGCTGTGTCAACAATTGTTAATAAAATCACCGAAGA
ATCGATTGGGTTGTAAAAATGGTCGCTATGGAGCACGTGAAGTTAAGTTATCATCATTCT
TTAGCGCCATCAATTGGAAACGACTTGAAGCAGGCATGGTGGAGCCCCCATTTATTCCAG
ACCCACATGCTGTTTACGCAAAAGATGTATTAGACATTGAACAATTTTCTACGGTGAAAG
GTGTTAATTTAGATGCTACTGATGAGAATTTCTATTCTAAATTCAATACCGGATCAGTGT
CGATACCATGGCAAAATGAAATGATTGAAACTGATTGTGTGCGAGAACTGAATATCTTTG
GTCCAGACGATAGTCCGACTCCCGATTTGATATTGAATGCACCCATTATTGAAGAAAAAC
AAGGATGCTTTCCATTTAGAAGAAAGAAAAAGCAACTTCCTCGAACAAAACCAATTCCCG
TACCCGAACATCTTTTGACACCTAGTCAAGCAGAAACAATAAGCGCAACAAATGAAAGCT
GA
>g2428.t2 Gene=g2428 Length=212
MIEGQAPFRARKEKVKREEVDRRVKEDAEKYSSKFSEEAKSLCQQLLIKSPKNRLGCKNG
RYGAREVKLSSFFSAINWKRLEAGMVEPPFIPDPHAVYAKDVLDIEQFSTVKGVNLDATD
ENFYSKFNTGSVSIPWQNEMIETDCVRELNIFGPDDSPTPDLILNAPIIEEKQGCFPFRR
KKKQLPRTKPIPVPEHLLTPSQAETISATNES
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g2428.t2 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 93 | 2.0E-17 |
| 7 | g2428.t2 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 96 | 168 | 2.0E-25 |
| 6 | g2428.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 23 | - |
| 5 | g2428.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 181 | 212 | - |
| 1 | g2428.t2 | PANTHER | PTHR24355:SF28 | G PROTEIN-COUPLED RECEPTOR KINASE 2 | 1 | 204 | 2.3E-99 |
| 2 | g2428.t2 | PANTHER | PTHR24355 | G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE | 1 | 204 | 2.3E-99 |
| 9 | g2428.t2 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 74 | 139 | 10.524 |
| 4 | g2428.t2 | SMART | SM00133 | pkinase_C_6 | 74 | 153 | 3.6E-11 |
| 3 | g2428.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1 | 154 | 6.47E-8 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
