General information for g2428.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative G protein-coupled receptor kinase 5.
Parent gene
- The parent gene g2428 is located on chr_3: 17928396..17931440 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2428 | g2428.t4 | isoform | g2428.t4 | 17927139 | 17931440 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon1 | 17927139 | 17927143 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS1 | 17927141 | 17927143 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon2 | 17927408 | 17927513 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS2 | 17927408 | 17927513 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon3 | 17928212 | 17928311 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS3 | 17928212 | 17928311 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon4 | 17928389 | 17928660 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS4 | 17928389 | 17928660 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon5 | 17928853 | 17928967 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS5 | 17928853 | 17928967 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon6 | 17929631 | 17929726 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS6 | 17929631 | 17929726 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon7 | 17930383 | 17930712 |
| chr_3 | g2428 | g2428.t4 | cds | g2428.t4.CDS7 | 17930383 | 17930434 |
| chr_3 | g2428 | g2428.t4 | exon | g2428.t4.exon8 | 17931143 | 17931440 |
| chr_3 | g2428 | g2428.t4 | TSS | g2428.t4 | 17931433 | 17931433 |
| chr_3 | g2428 | g2428.t4 | TTS | g2428.t4 | NA | NA |
Sequences
>g2428.t4 Gene=g2428 Length=1322
AGTACAGCCAAACTTCGATCGAGAGTGCAGAGCGATAAAATTTCTAGGCTTCAATCTCTT
CTCAATTTTTACTCAAATCTTATTAATTAAATCGAGTCAAAATAAATTTTTATTATTGTA
AAAGTGTAGTTTAAGGTGTAAAGATTTATATGTGGATAAGTGTGAAAAGTGTTTAGCAGA
CAAATTTGAGCTCAGAACATTTCAAAAATTATTCCAACCACCACACCTCGAAATTTTTTC
TTCTCCTCTCCTTCGTTCGCATTTCGCACACAAGAAATCAAAAAAAAATTATGAAGATGG
TTGTTGCGAGTTGAGAGTGTTTCGCGTGAGCTCATAAATTTGGAAAATATATAAAATAAA
AAAATTATCTAATAGAGGAAAGAGAAGAGTGGAGATTCGCATTGAGAGAGGAGCACAGAG
AAGTGTCTCGCTCACTTATTTTGGAAAAATTTCAATTTTTCAACGCAAAAATCTCGTAAA
CAGTGCTGCAGCAACGGAACCACTTGAGGTTCATCGTGAGCAATCTTTCTTTGGTTGAAG
GAGTGTTGAAAATAAAAGGCCGCCAACCTGGAACTGATGGAGTTAGAGAACATTGTGGCC
AATACGGTCTACCTCAAAGCCAGAGAGGGTGGCTCTGATAGTAACAAAGGAAAGAGCAAG
AAATGGCGCAAAATTTTACAATTTCCACACATATCTCAATGTATAGATCTCAAAAATAAA
ATAGATGTAAGCTATGATTATGTCGTAGACAAACAACCAATTGGAAGAGTATTGTTTCGG
CAGTTTTGTCAAGTCAAACAATTTTACAAATATGTAAAATTCTTAGAAGAAGTATCAAGG
TATGAGTTGGAGTCTGATGAAAATCGACAGGCAATTGCCATTAGAATAGGGCGACAATTT
TTGGGTATTGAAGAGGATGAAATTAAAGCGATAGATCAGGAGAATGAAAAAACTGACTCG
AACAATATCAATCCAAATAATGTTTCATCACCAAAAAACAACTCATCACAGCTCGATAAA
GCTGATGGCCAATCGGAACAGAATGAAATATCTCCCGTCACTGACATTCTAAATGATGAA
CTCATTTCAGACTGTAAGCAAAAACTTCACATCGCTTCAAAAGAGTTGTTTGAACCGTGC
TTTGTAGCAGTTAAAACGTATTTGGCAGGAGAGCCATTTAGAGAATTTGAGACTTCAATG
TATTTCCATAGATATCTTCAATGGAAATGGCTCGAAGCTCAGCCGGTTACATATAAAACT
TTTCGCATGTATCGAGTCCTGGGCAAGGGTGGTTTTGGTGAAGTGTGTGCTTGCCAAGTA
CG
>g2428.t4 Gene=g2428 Length=248
MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCIDLKNKIDVSYDYVVDKQ
PIGRVLFRQFCQVKQFYKYVKFLEEVSRYELESDENRQAIAIRIGRQFLGIEEDEIKAID
QENEKTDSNNINPNNVSSPKNNSSQLDKADGQSEQNEISPVTDILNDELISDCKQKLHIA
SKELFEPCFVAVKTYLAGEPFREFETSMYFHRYLQWKWLEAQPVTYKTFRMYRVLGKGGF
GEVCACQV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g2428.t4 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 227 | 248 | 1.7E-5 |
| 6 | g2428.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 121 | 153 | - |
| 7 | g2428.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 125 | 153 | - |
| 2 | g2428.t4 | PANTHER | PTHR24355:SF28 | G PROTEIN-COUPLED RECEPTOR KINASE 2 | 1 | 248 | 2.6E-82 |
| 3 | g2428.t4 | PANTHER | PTHR24355 | G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE | 1 | 248 | 2.6E-82 |
| 1 | g2428.t4 | Pfam | PF00615 | Regulator of G protein signaling domain | 53 | 213 | 6.4E-11 |
| 9 | g2428.t4 | ProSiteProfiles | PS50132 | RGS domain profile. | 59 | 214 | 19.536 |
| 5 | g2428.t4 | SMART | SM00315 | RGS_3 | 52 | 214 | 2.1E-14 |
| 4 | g2428.t4 | SUPERFAMILY | SSF48097 | Regulator of G-protein signaling, RGS | 31 | 224 | 1.43E-29 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
