General information for g2468.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Eukaryotic translation initiation factor 2-alpha kinase 1.
Parent gene
- The parent gene g2468 is located on chr_3: 18118060..18119357 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2468 | g2468.t4 | TSS | g2468.t4 | 18117434 | 18117434 |
| chr_3 | g2468 | g2468.t4 | isoform | g2468.t4 | 18118060 | 18119357 |
| chr_3 | g2468 | g2468.t4 | exon | g2468.t4.exon1 | 18118060 | 18118787 |
| chr_3 | g2468 | g2468.t4 | cds | g2468.t4.CDS1 | 18118106 | 18118787 |
| chr_3 | g2468 | g2468.t4 | exon | g2468.t4.exon2 | 18118852 | 18119357 |
| chr_3 | g2468 | g2468.t4 | cds | g2468.t4.CDS2 | 18118852 | 18119357 |
| chr_3 | g2468 | g2468.t4 | TTS | g2468.t4 | 18119507 | 18119507 |
Sequences
>g2468.t4 Gene=g2468 Length=1234
ACTTGAGAAAATATCCAGTGGCGGTTTTGGTGATGTTTACAAAGCGATGCACAATCTTGA
TAATAATATTTATGCGATAAAGAAAATTCTCATCAAATCTACATCAGTTAAGAACATTCT
TTCTCATTTAAGAGAAGTTAAAACTTTTGCTTCTCTAAATCATATTAATGTGGTACCTTA
CAAGAGTTGCTGGCTAGAACCTTTGATTTCATATGAACAAGAAAAGACAATATACGATGA
TGAAAGTTGGACAACAAAAAGTGAAAGTACTACCAATATAAGAATAACGAAAAGCAATGA
CAATTCATTACGCCTTCAAACCAACGACAGTTTTAGTATTAACTTTGAATACAGTGAAAA
CCATCAAAGTGAGCAAGTAAAAAGTGCAGATTCTTCAATTATGACAAATGGAGAAAGTCA
TTCTCAAGTGGAACGACTTAGAAAACTCAATTTATCAAAATCAAATGGAAGTACTTATTC
TTTAGCAATTGTTCCTCATGTAAAATTGGCATGGTCTGTTTTATACATCCAAATGAAGCT
ATGTCAAAAGACGCTGAGAAACTTTTTAGATGAGCGAAATGAACATGAATGCTTCAAAAA
GTATTATGAGAATGTTAATCTTCGTAGTGATAATCTTACTTACACAGCCTTAAGTATTTT
TAAGCAAATATGCAATGGATTAGAGTACATACATAGTAAATCAATAGTTCATCATGATCT
TAAGCCTGCAAATGTTTTTGTGTCATTTGAGAAATCTAATAATAAAGTAACATTCTCTAT
AGGAGATTTTGGACTAGCTTGTCCACTCGATAATAATGAGATGCTATCACACAATGGTAT
TGGAACGAGGCTTTATGCTGCGAAAGAGCAATTTGAAGGTCGAATTTGTTCAAAAAAGTC
GGACATTTATAGTTTAGGTGTCATTCTAATTGAACTTCTTTTACCTTGCAAAACAGTCAT
GGAATGTTTCAAGAAAGTAGAAAAAATCAAAAAAGGCGAATTATTACCAGAAATAGAAGC
CAACTTGTGTAAACTTATTGGCAGACTTCTGAGTCAACGAGCAGAATTGAGACCAGATGT
GAGCGAATTAAAAAGCTTAATACGTCATTTTATGGACAACACATCAGATGAAGTTGCGCG
TTTAAAAAATATTATCGAAGATAAAGATAAGCAAATTGATGATTTGATGCAAGAGATTAG
GAATTTAAAGAAACAGTTGTCAAAAGAAACATGA
>g2468.t4 Gene=g2468 Length=395
MHNLDNNIYAIKKILIKSTSVKNILSHLREVKTFASLNHINVVPYKSCWLEPLISYEQEK
TIYDDESWTTKSESTTNIRITKSNDNSLRLQTNDSFSINFEYSENHQSEQVKSADSSIMT
NGESHSQVERLRKLNLSKSNGSTYSLAIVPHVKLAWSVLYIQMKLCQKTLRNFLDERNEH
ECFKKYYENVNLRSDNLTYTALSIFKQICNGLEYIHSKSIVHHDLKPANVFVSFEKSNNK
VTFSIGDFGLACPLDNNEMLSHNGIGTRLYAAKEQFEGRICSKKSDIYSLGVILIELLLP
CKTVMECFKKVEKIKKGELLPEIEANLCKLIGRLLSQRAELRPDVSELKSLIRHFMDNTS
DEVARLKNIIEDKDKQIDDLMQEIRNLKKQLSKET
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g2468.t4 | Coils | Coil | Coil | 356 | 395 | - |
| 4 | g2468.t4 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 1 | 94 | 2.1E-12 |
| 5 | g2468.t4 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 198 | 383 | 4.6E-39 |
| 2 | g2468.t4 | PANTHER | PTHR11042 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED | 2 | 352 | 3.0E-64 |
| 1 | g2468.t4 | Pfam | PF00069 | Protein kinase domain | 159 | 349 | 3.5E-27 |
| 7 | g2468.t4 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 220 | 232 | - |
| 9 | g2468.t4 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 356 | 26.534 |
| 8 | g2468.t4 | SMART | SM00220 | serkin_6 | 1 | 356 | 1.1E-10 |
| 3 | g2468.t4 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 2 | 351 | 3.38E-45 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
