General information for g2599.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Trypsin-4.
Parent gene
- The parent gene g2599 is located on chr_3: 18994256..18996558 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2599 | g2599.t1 | isoform | g2599.t1 | 18993395 | 18996558 |
| chr_3 | g2599 | g2599.t1 | exon | g2599.t1.exon1 | 18993395 | 18993508 |
| chr_3 | g2599 | g2599.t1 | cds | g2599.t1.CDS1 | 18993395 | 18993508 |
| chr_3 | g2599 | g2599.t1 | exon | g2599.t1.exon2 | 18993562 | 18993790 |
| chr_3 | g2599 | g2599.t1 | cds | g2599.t1.CDS2 | 18993562 | 18993790 |
| chr_3 | g2599 | g2599.t1 | exon | g2599.t1.exon3 | 18994256 | 18994819 |
| chr_3 | g2599 | g2599.t1 | cds | g2599.t1.CDS3 | 18994256 | 18994819 |
| chr_3 | g2599 | g2599.t1 | exon | g2599.t1.exon4 | 18996398 | 18996558 |
| chr_3 | g2599 | g2599.t1 | cds | g2599.t1.CDS4 | 18996398 | 18996558 |
| chr_3 | g2599 | g2599.t1 | TTS | g2599.t1 | 18997172 | 18997172 |
| chr_3 | g2599 | g2599.t1 | TSS | g2599.t1 | NA | NA |
Sequences
>g2599.t1 Gene=g2599 Length=1068
ATGACAGATACTTCTTGTTTAGTTTGTTTTAATAACGAAAGTAATATTAAATACATTTCA
TTAAAAGAGAATAAAGATTTGTTAAAAAAATTTGAAGAATGCTTCAAAACCAACGTTGAT
GAAAAAGTATTAATATGCGAAAGATGTTTAGATCTAGTCATTCAATTTTGGGAGATGAAA
AATCGTGTACAAGTTTTAGATAAGTCTTATTCTAAAGCTTTACGAAAACAAAAAATGTCA
GAAGAAACTGCTAAAACTATAACTAATAAAATGAAACAATCAAAGAAGAAAATGAGAAGA
AACACATTAGATGAAAGAAAATTTAACCGAAAACAAATTAAGAAAGAAAATGAATTTGGT
GAAGGATTAGAAAGAATAGTGGGAGGACAAATTATGCAAATTGAAAGTGTTCCATATATG
GTATCAATATTTTACAAAAACAATCATCTTTGCGGCGGGTCTATTATCTCTAGACAATGG
ATTATATCTGCAGCACACTGTGTTGTAGATACAGACGCGAAAAATTATTGGGTGAGAAGT
GGATCTTCAAGGAAATCACGAGGTGGAACTGTTACAAACGCAGAGAAAATAATTCCTCAT
CCCGATTATGATACAAAAACGCTTAACTATGATTTCATGCTTCTTAAAATTTCACATCCA
CTCATCTATTCAGAACGACAGCAGCCAATCAAAATTGCAGACGATTCATCACGAGCTTAT
TTTGCAGGAGAAGAAGTTTTGACTAGCGGTTTTGGACTGACTCAAAATGATATGGAATCT
AATGAATTTTTGCGTGGAGTTATTGTCAAATTTTCAAGTAAAAATGAATGTTTAAAAGCA
TATCCAGAAATAATCACCGAAAATATGGTTTGTGCTGGCTCCCAAAATAATCAAGATTCT
TGTCAAGGTGATAGTGGTGGGCCTTTGGAATCACTCAGAACAGGTGAATTAGTCGGAATC
GTCAGCTTCGGAATGTCATGTGCAGACCCTTCATATCCTGGTGTATATTCAAAAGTATCA
AAAGTGAAAGACTGGATTGAACAAACGAAATCTGAAGTTGATAATTGA
>g2599.t1 Gene=g2599 Length=355
MTDTSCLVCFNNESNIKYISLKENKDLLKKFEECFKTNVDEKVLICERCLDLVIQFWEMK
NRVQVLDKSYSKALRKQKMSEETAKTITNKMKQSKKKMRRNTLDERKFNRKQIKKENEFG
EGLERIVGGQIMQIESVPYMVSIFYKNNHLCGGSIISRQWIISAAHCVVDTDAKNYWVRS
GSSRKSRGGTVTNAEKIIPHPDYDTKTLNYDFMLLKISHPLIYSERQQPIKIADDSSRAY
FAGEEVLTSGFGLTQNDMESNEFLRGVIVKFSSKNECLKAYPEIITENMVCAGSQNNQDS
CQGDSGGPLESLRTGELVGIVSFGMSCADPSYPGVYSKVSKVKDWIEQTKSEVDN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g2599.t1 | CDD | cd00190 | Tryp_SPc | 126 | 349 | 1.65608E-87 |
| 8 | g2599.t1 | Gene3D | G3DSA:2.40.10.10 | - | 121 | 352 | 5.5E-67 |
| 2 | g2599.t1 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 113 | 352 | 2.6E-77 |
| 3 | g2599.t1 | PANTHER | PTHR24276:SF83 | GH13245P2-RELATED | 113 | 352 | 2.6E-77 |
| 6 | g2599.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 152 | 167 | 7.4E-15 |
| 5 | g2599.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 207 | 221 | 7.4E-15 |
| 4 | g2599.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 298 | 310 | 7.4E-15 |
| 1 | g2599.t1 | Pfam | PF00089 | Trypsin | 126 | 346 | 5.8E-60 |
| 10 | g2599.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 162 | 167 | - |
| 12 | g2599.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 126 | 351 | 31.351 |
| 11 | g2599.t1 | SMART | SM00020 | trypsin_2 | 125 | 346 | 1.4E-76 |
| 7 | g2599.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 123 | 349 | 3.28E-74 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
- This transcript has been annotated with K01312 (trypsin)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
