General information for g2685.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Endoplasmic reticulum chaperone BiP.
Parent gene
- The parent gene g2685 is located on chr_3: 19712637..19714329 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2685 | g2685.t8 | isoform | g2685.t8 | 19711137 | 19714329 |
| chr_3 | g2685 | g2685.t8 | exon | g2685.t8.exon1 | 19711137 | 19713361 |
| chr_3 | g2685 | g2685.t8 | TTS | g2685.t8 | 19711154 | 19711154 |
| chr_3 | g2685 | g2685.t8 | cds | g2685.t8.CDS1 | 19711516 | 19713045 |
| chr_3 | g2685 | g2685.t8 | exon | g2685.t8.exon2 | 19713513 | 19714329 |
| chr_3 | g2685 | g2685.t8 | TSS | g2685.t8 | NA | NA |
Sequences
>g2685.t8 Gene=g2685 Length=3042
ATGAAAAGTTTGAATGGTTTGAATTTTACATATTACGTTAATTTTTAAATCAAAAATTTT
ATGTCATCCAAAATATGATGAAATATGACCAAGTTTCTCAATAAAACATCCTCTCAAATT
CAAAAATACTCTTTGAAATAGGTTGAAAATGGAACAAAAGAACACGTCAGTATAGAAAAG
AGAGATAGTATAAATTGTGAGCAATGAGACCAAATTTCAAGTCATTTCACTAAAAGGTTT
CACAGAGTAAAGACATCAAAAAAAAGATTGAGTAAAAAATTGAGTTATTTAAAGTGATTT
ATCTAGTGAAGTTAAGTAAATTTAATAATTTTGAATTATCAAATAAGAGTAGTTAAGCTA
TTAAAAGTAAGTGAAAAGTCAAATTATAGTGAAAATAAATTATTTTCATTGTGAAATTTA
AGAAAGAACATTTTCCTTTCAAAAAGCCGAAGAACAGAAAAGCCACGTCATTCATAAAGT
TTTTCTAAAAAGTTGTATTAAAATTTAATCATATTTGTGATTTAAATGATTTTAATTAAT
CAATTCATTGGAATACACATGATTTCATATTAAAAACTAGTTAATTTTACAACGAGTTTT
CACATTATATCACATTTTTACTAGAAACTTCTTCCTAACATCTTTTCTCTCTCTCTTTGT
CTTATTAGGTAGAAACAAAATTAAAATGAGATTAACTCTATCATTCGTGGTCGTGGCGGC
ATTCAGTGTGCTGGGAGTTCTTTCGAGCGAGGATAAGGATAAAAAAGATATTGGTACAGT
GATCGGTATCGATTTGGGAACGACATATTCATGGTAGTGTCGGAGTATATAAGAATGGAC
GTGTAGAAATTATTGCAAATGATCAAGGAAATCGTATCACACCTTCATATGTGGCATTCA
CTTCTGAAGGAGAACGATTGATTGGTGATGCAGCTAAAAATCAGCTCACAACAAATCCAG
AAAATACTGTGTTCGATGCTAAACGTCTAATTGGTCGTGAATGGAGTGACTCAGCTGTTC
AACATGATATCAAATTTTTCCCATTCAAAGTTGTCGAGAAGAACTCAAAGCCACACATTG
AAGTGAAAACTCAACAAGGCAACAAAGTCTTCACACCTGAAGAAATTTCAGCTATGGTTC
TTGGAAAAATGAAAGAAACTGCTGAGGCATATTTGGGCAAAAAAGTTACGCACGCTGTTG
TCACAGTTCCAGCTTATTTCAATGATGCTCAACGTCAAGCCACAAAAGATGCTGGTATCA
TCTCTGGATTGAATGTTATGCGTATTATTAATGAACCTACTGCAGCAGCTATTGCATATG
GGCTTGATAAAAAAGACGGCGAAAAGAATGTTTTAGTATTCGATCTTGGTGGTGGTACAT
TCGATGTCTCGCTTCTTACAATTGATAACGGTGTTTTCGAAGTTGTTGCCACAAATGGTG
ATACTCACTTAGGAGGTGAAGATTTTGATCAACGTGTGATGGATCATTTCATTAAATTGT
ACAAGAAGAAGAAGGGCAAGGACATTCGTAAAGATAATCGCGCTGTACAGAAACTTCGTC
GTGAAGTTGAAAAGGCTAAACGTGCTCTCTCAGCTAGTCAACAAGTTCGTATTGAAATAG
AATCATTCTTTGAAGGAGAAGACTTTAGCGAATCACTTTCACGTGCCAAATTTGAAGAAT
TGAATATGGATTTGTTCAGATCAACTTTGAAACCAGTTCAAAAAGTCTTGGAAGATGCTG
ACATGAATAAGAAAGATGTTGATGAAATTGTTTTGGTCGGTGGTTCAACACGTATTCCAA
AAGTTCAGCAATTGGTCAAAGAATTCTTCAATGGAAAAGAACCATCACGTGGCATCAATC
CAGATGAAGCTGTTGCTTATGGTGCTGCTGTTCAAGCTGGTGTTCTTTCAGGCGAACAAG
ACACAGACGCTATCGTTCTTCTCGATGTCAATCCTTTGACTATGGGTATTGAAACAGTCG
GAGGTGTGATGACAAAATTGATTCCAAGAAATACAGTTATTCCAACAAAGAAATCTCAAA
TTTTCTCCACTGCCAGTGATAATCAACATACTGTAACAATTCAAGTTTATGAAGGTGAAC
GTCCAATGACCAAAGACAATCACTTATTAGGTAAATTCGATCTTACTGGCATTCCTCCTG
CTCCACGTGGAATCCCACAAATTGAAGTTTCATTTGAAATCGATGCTAATGGTATTCTTC
AAGTATCAGCTGAAGATAAGGGCACAGGAAATCGCGAGAAAATTGTAATCACAAATGATC
AAAACAGACTCACACCAGAAGATATTGAACGTATGATTAAGGATGCTGAGCGTTTTGCTG
ATGATGACAAGAAACTCAAGGAACGTGTCGAGGCACGTAATGAACTTGAGAGTTACGCAT
ACAGTCTGAAAAATCAAATTGGAGATAAGGAAAAACTTGGAGCAAAATTGTCAGATGATG
AAAAGAGCAAAATGGAAGAGGCTATTGACGAAAAGATCAAATGGTTAGAAGAAAATCAAG
ATACAGATGCTGAAGATTACAAGAAACAAAAGAAAGAACTTGAAGAAGTTGTGCAGCCAA
TTATCGCTAAATTATATCAAGGACAAGGTGGTGTACCACCACCGACAGGCGATGATGATG
ATGATCTCAAAGACGAATTGTAAAAAAGCTCCCAAAAATTCCTTCCCTTCGTACAACATA
CACAAACAATATTTTCCTGAAATCAACCTCAATTTTTTTAATGTTAAATTGTTTATTGTA
AAAGCTACATTCATTTGTTAATTGAAAGTCATTTTCTCTTTTAATTTTCATTCCGTTCTA
TATCCGTTCTCAAAAAAAAATTCTTTTCATAGGTCGCATAAAAAAATACATTTTCCTAGA
ATTTAATTTATTAGTAGAAGAAAAAAGAAATTTACAACCAATGCACATGACTGAATGAGC
GAATGCGTGCGTGATCTTAATTTTGTAATGCATTAGTTTCTATTATGAAACAAATTGAAT
AAAAAACTAAACTAGTAAAACTTAGAAAATAATAGTTCTTTT
>g2685.t8 Gene=g2685 Length=509
MVLGKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGIISGLNVMRIINEPTAAAI
AYGLDKKDGEKNVLVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMDHFI
KLYKKKKGKDIRKDNRAVQKLRREVEKAKRALSASQQVRIEIESFFEGEDFSESLSRAKF
EELNMDLFRSTLKPVQKVLEDADMNKKDVDEIVLVGGSTRIPKVQQLVKEFFNGKEPSRG
INPDEAVAYGAAVQAGVLSGEQDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKK
SQIFSTASDNQHTVTIQVYEGERPMTKDNHLLGKFDLTGIPPAPRGIPQIEVSFEIDANG
ILQVSAEDKGTGNREKIVITNDQNRLTPEDIERMIKDAERFADDDKKLKERVEARNELES
YAYSLKNQIGDKEKLGAKLSDDEKSKMEEAIDEKIKWLEENQDTDAEDYKKQKKELEEVV
QPIIAKLYQGQGGVPPPTGDDDDDLKDEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 20 | g2685.t8 | Coils | Coil | Coil | 131 | 158 | - |
| 19 | g2685.t8 | Coils | Coil | Coil | 459 | 479 | - |
| 16 | g2685.t8 | Gene3D | G3DSA:3.30.420.40 | - | 1 | 94 | 3.6E-37 |
| 15 | g2685.t8 | Gene3D | G3DSA:3.90.640.10 | Actin; Chain A | 107 | 181 | 4.0E-25 |
| 17 | g2685.t8 | Gene3D | G3DSA:3.30.420.40 | - | 182 | 237 | 4.9E-12 |
| 14 | g2685.t8 | Gene3D | G3DSA:2.60.34.10 | Substrate Binding Domain Of DNAk; Chain A | 253 | 384 | 6.1E-59 |
| 18 | g2685.t8 | Gene3D | G3DSA:1.20.1270.10 | - | 386 | 490 | 4.7E-35 |
| 23 | g2685.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 486 | 509 | - |
| 2 | g2685.t8 | PANTHER | PTHR19375 | HEAT SHOCK PROTEIN 70KDA | 1 | 495 | 0.0 |
| 3 | g2685.t8 | PANTHER | PTHR19375:SF380 | ENDOPLASMIC RETICULUM CHAPERONE BIP | 1 | 495 | 0.0 |
| 4 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 21 | 41 | 1.7E-62 |
| 7 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 81 | 91 | 1.7E-62 |
| 9 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 209 | 225 | 1.7E-62 |
| 8 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 241 | 261 | 1.7E-62 |
| 6 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 266 | 285 | 1.7E-62 |
| 5 | g2685.t8 | PRINTS | PR00301 | 70kDa heat shock protein signature | 347 | 363 | 1.7E-62 |
| 1 | g2685.t8 | Pfam | PF00012 | Hsp70 protein | 1 | 489 | 3.9E-213 |
| 22 | g2685.t8 | ProSitePatterns | PS00329 | Heat shock hsp70 proteins family signature 2. | 75 | 88 | - |
| 21 | g2685.t8 | ProSitePatterns | PS01036 | Heat shock hsp70 proteins family signature 3. | 212 | 226 | - |
| 12 | g2685.t8 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 1 | 66 | 6.8E-25 |
| 13 | g2685.t8 | SUPERFAMILY | SSF53067 | Actin-like ATPase domain | 68 | 260 | 2.07E-67 |
| 11 | g2685.t8 | SUPERFAMILY | SSF100920 | Heat shock protein 70kD (HSP70), peptide-binding domain | 263 | 419 | 3.14E-62 |
| 10 | g2685.t8 | SUPERFAMILY | SSF100934 | Heat shock protein 70kD (HSP70), C-terminal subdomain | 413 | 495 | 7.59E-26 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09490 (endoplasmic reticulum chaperone BiP)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
