General information for g2703.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Piwi-like protein Siwi.
Parent gene
- The parent gene g2703 is located on chr_3: 19775002..19776689 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2703 | g2703.t3 | isoform | g2703.t3 | 19775002 | 19776116 |
| chr_3 | g2703 | g2703.t3 | exon | g2703.t3.exon1 | 19775002 | 19775263 |
| chr_3 | g2703 | g2703.t3 | cds | g2703.t3.CDS1 | 19775002 | 19775263 |
| chr_3 | g2703 | g2703.t3 | exon | g2703.t3.exon2 | 19775320 | 19775641 |
| chr_3 | g2703 | g2703.t3 | cds | g2703.t3.CDS2 | 19775320 | 19775641 |
| chr_3 | g2703 | g2703.t3 | exon | g2703.t3.exon3 | 19775701 | 19776116 |
| chr_3 | g2703 | g2703.t3 | cds | g2703.t3.CDS3 | 19775701 | 19776064 |
| chr_3 | g2703 | g2703.t3 | TSS | g2703.t3 | NA | NA |
| chr_3 | g2703 | g2703.t3 | TTS | g2703.t3 | NA | NA |
Sequences
>g2703.t3 Gene=g2703 Length=1000
CAAAGGAGTAAAGAGCGGTCGTCAAGAAGAGTACACAAGTATCATTGAAGAGATGGCTGA
AAAGAAACCCAATTTCGTATTGTGTATTTTACCAACAAATCGTGCCGACAGATATAGTGC
ATTGAAACGTTTGCTTATTGTGAAATTTGGTATACCTAGTCAAGTAATAGTTGAGAATCG
TGTGTTGAAACATAAGAATTTGCGTTCGATCTGTACTAAAGTGGCAATTCAAATCAATTG
TAAGCTTGGGGGGATCCCTTGGCTTATTAAAATGCCGGTAGCCGGCTTGATGGCGGTTGG
TTTCGATGTAAGTTATGATCCACGAAATCGAAAGCGAAGTATTGGAGCAATGGTTGCAAC
TATGGATGTTCGAGTTTCTTCTTGCTTCTATTCTGTTACTGTGCAATATAAAGATGGAAA
TGAAATGGTTGAAAAAATTGATCAACATATGCAAAAGGCGATTGAATTTTACAAGTATGC
AGTTGGTGCTTTTCCTGAGAGAATTGTATATTATCGTGATGGTATTGGTGATTCACAAAT
TCAATTTGTAAAGAGACAGGAAGTAGAACCTATTATCAAGACTTTGAGTCAAATTTATGG
AAGCAATCCTCGATTTGCTTTCATTGTTGTTAACAAACGAACCAATGCACGATTCTTCAA
AAAACAAGGCAATCAATTTATCAATCCGCAACCAGGTTCTATTATTGATAACGGTGTCAC
ATGTAAAGGTCGTCAAGATTTTTATTTAATTTCTCAACATGTTGGACAAGGTACTGTAGC
TCCGACTTATTACAACATCATACATAACAATACTGGTTTGGATATAGAAAAAGTCGAATT
ATTAACTTATAAATTATGCCATTTATATTACAATTGGGGTGGCACTGTTCGTATTCCAAG
CATCTGTCAAAATGCAAAGAAACTTGCATTTTTAACCACACAATCTCTTAATGGTGCAAT
TGCTAATGAACATTTAACTAAGAGCTTATACTTTTTGTAA
>g2703.t3 Gene=g2703 Length=315
MAEKKPNFVLCILPTNRADRYSALKRLLIVKFGIPSQVIVENRVLKHKNLRSICTKVAIQ
INCKLGGIPWLIKMPVAGLMAVGFDVSYDPRNRKRSIGAMVATMDVRVSSCFYSVTVQYK
DGNEMVEKIDQHMQKAIEFYKYAVGAFPERIVYYRDGIGDSQIQFVKRQEVEPIIKTLSQ
IYGSNPRFAFIVVNKRTNARFFKKQGNQFINPQPGSIIDNGVTCKGRQDFYLISQHVGQG
TVAPTYYNIIHNNTGLDIEKVELLTYKLCHLYYNWGGTVRIPSICQNAKKLAFLTTQSLN
GAIANEHLTKSLYFL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g2703.t3 | CDD | cd04658 | Piwi_piwi-like_Euk | 4 | 297 | 0.000 |
| 7 | g2703.t3 | Gene3D | G3DSA:3.40.50.2300 | - | 1 | 64 | 0.000 |
| 6 | g2703.t3 | Gene3D | G3DSA:3.30.420.10 | - | 74 | 314 | 0.000 |
| 2 | g2703.t3 | PANTHER | PTHR22891 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2C | 5 | 315 | 0.000 |
| 3 | g2703.t3 | PANTHER | PTHR22891:SF164 | PIWI-LIKE PROTEIN 1 | 5 | 315 | 0.000 |
| 1 | g2703.t3 | Pfam | PF02171 | Piwi domain | 8 | 299 | 0.000 |
| 8 | g2703.t3 | ProSiteProfiles | PS50822 | Piwi domain profile. | 8 | 300 | 41.944 |
| 5 | g2703.t3 | SMART | SM00950 | Piwi_a_2 | 8 | 300 | 0.000 |
| 4 | g2703.t3 | SUPERFAMILY | SSF53098 | Ribonuclease H-like | 4 | 315 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003676 | nucleic acid binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
