General information for g2739.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial.
Parent gene
- The parent gene g2739 is located on chr_3: 19939642..19940855 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2739 | g2739.t5 | TSS | g2739.t5 | 19938544 | 19938544 |
| chr_3 | g2739 | g2739.t5 | isoform | g2739.t5 | 19938614 | 19940855 |
| chr_3 | g2739 | g2739.t5 | exon | g2739.t5.exon1 | 19938614 | 19939566 |
| chr_3 | g2739 | g2739.t5 | cds | g2739.t5.CDS1 | 19939253 | 19939566 |
| chr_3 | g2739 | g2739.t5 | exon | g2739.t5.exon2 | 19939627 | 19940855 |
| chr_3 | g2739 | g2739.t5 | cds | g2739.t5.CDS2 | 19939627 | 19940104 |
| chr_3 | g2739 | g2739.t5 | TTS | g2739.t5 | 19940861 | 19940861 |
Sequences
>g2739.t5 Gene=g2739 Length=2182
ATGCAAGCGACAAGATTTTTCAAGAAATTGGTAAGTTAAGTAAACGACATCATTTGAATG
TCATTCATTAATAAACGCAACATTGAAATCTAGAAATTTTCTAGGAATTTTTTTTTAATT
GTGCATGAACAGTAAATATCTTGTGCAATTTCTAAAATTTTCTACAAGTTCTTAGTATAA
CATAAAGAAAAATATAATCAAAATCACTCATTTCAAAGAGCTTTTTACTAAATGATCCTT
TAAATATTTCAAAATATTGAAAAAATCAAGAAAATTTGTTACGCAATATTGACCTTATAA
TTATAATGTATTAAAAATGTCACTTTAATATCTTCTAATAACAACACAAACAATGATGAG
GACATAAATATCTGTTTTTCATTTTAATTTTAGATCACGCCATCAGGATGTGTAAGAAAT
TTTGCTTCGCAGACAAAGAAAGTAACATTGATTCCAGGCGATGGAATCGGACCGGAAATC
AGTGCAGCTGTGCAAAAGATTTTCGCAACTGCTCAAGTCCCAATTGAATGGGAAAGTGTT
GATGTGACGCCTGTAAGAAATCCCGATGGTCGTTTCGGTATTCCTCAAGCTGCAATTGAT
TCGGTTAATCGAAATAAAGTTGGCTTAAAAGGGCCATTGATGACACCAGTCGGCAAGGGA
CATCGATCATTGAATTTAGCTCTTAGAAAAGAATTTGATCTCTATGCAAATGTTAGACCT
TGCAGAAGCCTTGAAGGCTACAAAACTCTCTATGATGACGTCGATGTTGTTACAATCAGA
GAAAATACTGAAGGCGAGTACTCTGGTATTGAGCACGAAATTGTTGATGGTGTCGTTCAA
AGTATCAAATTGATTACCGAAGAGGCCTCAAAACGTGTTGCCGAATATGCCTTCCAATAT
GCAAAGGAAAATCATCGTTCAAAAGTAACTGTCGTACACAAAGCTAATATCATGCGTATG
TCTGATGGTCTTTTCTTACGTTGTTGTCGTGAGACTGCTGATAAATTTCCAGAAATTAAA
TTTGAGGAAAGATACCTTGATACTGTCTGCTTAAATATGGTACAAGATCCACGTCAATAT
GATGTTCTTGTTATGCCAAACTTGTATGGTGATATTTTGTCAGACATGTGTGCTGGCTTA
GTTGGTGGTCTCGGACTTACACCCTCTGGAAATATTGGTCTTGGTGGTGCACTTTTTGAA
AGTGTTCATGGTACAGCTCCTGATATTGCTGGCAAGGATTTGGCAAATCCAACTGCTCTT
CTTCTGTCGGCTGTTATGATGCTTCGTCATATGGAAATGAACGATAAAGCTGATAAAATT
CAGAAAGCATGCTTCGAGACCATAAAAGAAGGCAAATATCTCACTGGAGATTTAGGTGGA
AAGGCAAAGTGCTCTGAATTTACAAATGCTATTTGTGAAAAATTAGCTTAATTATTAGGT
ATTCAATTCGGATTAGATATGAAGCTCTACGTTTCTTCAGCGATCTCCATTTTTTTTAAA
AATTGATCCTACTTAATTGCATGTCCTAAGTTTAGTTGTTATCATAGATAACAAATGAAT
CTTAATATCAAAGAAATTTTTTAGATTATCCAAAAAAAACGCTTTAAATAACCTAAGTTC
TTTCAACGAATATTAATAAGTTATAACATCTCCCAAAAGTGTTTGATAGTCAAATGATCC
CACCTAATTTCGCATCCTTCCCTCAAAATTATTATAACCATGTCCTTATCATTGCGAAGG
AGAAATGTAGACATAATAAGTAACATCTTAACACAAAAAATACAATGTTTTTGCATTGTC
AACTGTATGACTATTATTGAAAAACGGAAAAAGAAAGAGATGATTAAGTTGTAAATTGAA
AAAAAAAATTAATTTATTTAATACAACACAATAATTATTGTTAAACAGGTTTAAATATTA
TACACATAGCCTCTCAATAGATGCAAAAAAATATGCAATAATTTATAAATTATTTGATAT
TTAATCTCAATTAGATAGATCCGTCATTATCCGTTATTAATGGTGACTGATTCGATAGAG
CACAGAATTCGTATTTAGCGAAATCTCTTAAAGATATTTTTGATCGACGTATGAAGTTTT
AGTTGTTTCTTAGAAATAAAACAATGAAATTTCATTTGAATCTGAACAAAAAAGACAATA
AATTCATTGATTGGACATTATT
>g2739.t5 Gene=g2739 Length=263
MTPVGKGHRSLNLALRKEFDLYANVRPCRSLEGYKTLYDDVDVVTIRENTEGEYSGIEHE
IVDGVVQSIKLITEEASKRVAEYAFQYAKENHRSKVTVVHKANIMRMSDGLFLRCCRETA
DKFPEIKFEERYLDTVCLNMVQDPRQYDVLVMPNLYGDILSDMCAGLVGGLGLTPSGNIG
LGGALFESVHGTAPDIAGKDLANPTALLLSAVMMLRHMEMNDKADKIQKACFETIKEGKY
LTGDLGGKAKCSEFTNAICEKLA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g2739.t5 | Gene3D | G3DSA:3.40.718.10 | Isopropylmalate Dehydrogenase | 1 | 263 | 1.4E-123 |
| 2 | g2739.t5 | PANTHER | PTHR11835 | DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE | 1 | 260 | 1.0E-150 |
| 3 | g2739.t5 | PANTHER | PTHR11835:SF34 | ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL | 1 | 260 | 1.0E-150 |
| 1 | g2739.t5 | Pfam | PF00180 | Isocitrate/isopropylmalate dehydrogenase | 2 | 258 | 1.4E-93 |
| 6 | g2739.t5 | ProSitePatterns | PS00470 | Isocitrate and isopropylmalate dehydrogenases signature. | 154 | 173 | - |
| 5 | g2739.t5 | SMART | SM01329 | Iso_dh_2 | 1 | 258 | 5.8E-121 |
| 4 | g2739.t5 | SUPERFAMILY | SSF53659 | Isocitrate/Isopropylmalate dehydrogenase-like | 1 | 262 | 5.58E-106 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000287 | magnesium ion binding | MF |
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | MF |
| GO:0051287 | NAD binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K07246 (tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
