General information for g2782.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Prosaposin.
Parent gene
- The parent gene g2782 is located on chr_3: 20266523..20273503 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2782 | g2782.t1 | TSS | g2782.t1 | 20263032 | 20263032 |
| chr_3 | g2782 | g2782.t1 | isoform | g2782.t1 | 20263126 | 20266523 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon1 | 20263126 | 20263162 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS1 | 20263126 | 20263162 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon2 | 20263266 | 20263359 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS2 | 20263266 | 20263359 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon3 | 20263789 | 20265410 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS3 | 20263789 | 20265410 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon4 | 20265464 | 20265576 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS4 | 20265464 | 20265576 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon5 | 20265636 | 20266218 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS5 | 20265636 | 20266218 |
| chr_3 | g2782 | g2782.t1 | exon | g2782.t1.exon6 | 20266291 | 20266523 |
| chr_3 | g2782 | g2782.t1 | cds | g2782.t1.CDS6 | 20266291 | 20266523 |
| chr_3 | g2782 | g2782.t1 | TTS | g2782.t1 | 20266737 | 20266737 |
Sequences
>g2782.t1 Gene=g2782 Length=2682
ATGTGGAAGCTAAATTACTTTATTGTGTTATTATGTGTGGTATCATCATCATCATCATTA
GCAAATAATGTATCACAGAAATATGATGCCGAATGTTCGAAGGGAATTACTTTTTGGTGT
GAGCATTTAATGAATGCAAAAAAATGCAACGCAGTTGATTTCTGTATTAAAACTGTTTGG
GAAAATCATCTTGTGAAGATCGATTCAAATCCACTTTGTGATGACTGCAAAGATTGGGTG
AAGCAAGCTCGAGATTTGTTGGAACGAAAAGAAACTGCAAATGCAATTATAAGAACTTTA
GCGTGGTCATGTGCATTATGTCCAGATACCAATGGAAGGACAAAATGTAGACAAATTATT
TCTAATAATGTGATGGAAATTCTGAAACTTCTTGACTCACGAATGAATCCCGATGCAATT
TGTTCAGCAATTCATTTCTGCAATAATCGTGAATTTACAAAAATATTTGAAAAGGCATTT
GAGTTAGAATCTCAACAAAAATTGCAGCAAAAACCTAGACAAAAGTTGCTACCTTTTACT
TGTGGTCAGTGCAATCATATTGGATCAGTTCTAGGAGAAAAGCTCGCGAATGCAAATCAC
GATGATATGCTCGAGGGTGTGTTGAAAGTTTGCGGTCAAATGTCATCATTTTCTGATGCA
TGTTCAAACCTCGTATTCAAAAACTTCAATGATATCTACCAGCAACTCCCAAAACTAATA
ACGAAAGAGAAACTATGTCATTCATCATGTTCACAACATTATCAAAATCATGAAGGCATC
GTTGATATTTCACCAGCATTTGACAATCCAAATATTCCATGTGAATTATGTGAACAGCTC
ATGCTCCATCTTCGAGAAGTTTTAATAGCAAACACTACAGAAATTGAGTTTAAGAATATC
CTTGAAGGTTTCTGCAATCAAATGCCTTCCATTGCACAAGAATGTGTTTCACTTGCTGAT
CAATATTATGACTCAATTTATCAATATCTTGTGAATGGACTTGATGCAAATAAAACCTGT
GTCATGATCGGAATATGCTCACATAAATTAGATTCGTATAATGCACCAGCAATGCCACTT
TTATCAAGTGAATTCTTCCCAGTACCAGAAGTAGATTCAATCGAAGTTCATATTGTAACA
CAGGATTCATCAATAAATCTTCATAAGGATGGAAGATTGTGTACTGCATGCGAATATACA
ATGAATTTAGTTCACATCGAAATGAATAAGAATGGAGTACAAGAAAACATTTTAAAGAAA
GTCAGAGCAGAATGTAAAAAGTTACCAGCTTATGTACGTCAATGTGAAGATCTTATTGAT
ACGTTTGGTGATCAAATTATGAATGCAATTTATCAAGGCACAAATCCACGTTTAGTTTGT
CCACTCATAAAAATGTGTCCTCCCAATTTAAACTTTCAATACCTCCAAGAAACTGCTGTA
GATGAAAAACCAACATGTCCATTCTGTCTTTTCGCTATGCAAGAGGTTCGAGATTTGGTA
TCGTCAAATGCATCCAAAACAAATGTTGAAAAAGTTTTAGATCAACTATGCACGCATCTA
TCAGACAAATTAAAATCACAATGTATTGAGTTTGTTAATGAATATTCATCAGAAGTGGTA
GACATGATTTTAGCAAACTTCACACCACAAGAGGCATGTGTCTTTATAAAATTGTGTAGT
CAAGAGAAACCGAAATTAAAACGTTTTAGTATGGCTGGAATTTTTAATGAAAATCATAGC
GATGAAATGATAGATTTTGATGATGATGATAATAACAATTCAAAGTTACTTGATAATCCT
CAATGTGATTTGTGTAAAGCTGTTATAAAATTGATTGAACAAAGAGTCATTGATATTAAA
TCAAAGGATGAAATTCGTCGTGAACTTGAAAATTCTTGTAGTCATTTAAAACGGTTTTCA
AAAGAGTGCAAAGCATTTGTCGACAAATATTCTGATCGAATTGTTGATTTAGTTAGCAAG
GAATTAGCACCCGAAAATGTATGCAAAGAACTGATATTTTGTGTAACTGAAGAGAAGGTT
GACATGCAAGATTATGATGTCGGTCTTGATATTTTCGCAAAAAGTTTCAGTGAATCAGCA
GAAGTCAGTGAAGACCTTGAAGTTACAGGAACGAGTTGTATCATTTGTGAATTTATTATG
ACTAAAATCGATGAAGAATTAAATGATAAGCAAAAGGATGAAGATATTAAACACATCATT
AAAAACGTTTGCTCAAAAATGCCATCAACTGTTTCGAAGCAATGTAATCAATTTATCGAC
TATTACTTTGACATGATCATTGTACTCATCGAGACAACGAAACCATCAGAAATGTGTAAA
GAGTTGAAGCTTTGTCCAAAATCAAATGAAGAACTTGAAAATCAACTTGTGGAAATAAAA
GGTGACATTTATACCTGTGCAGTTTGCAGAGGTGCTGTTGAGAGTTTAGATTCTATAATT
GAAGATCCACAAGTTGATGTAAAATTGGAAAATCTTGAAGAAAAAATTTGTGAAAAATTT
GCAGGAAAATTTAAAGAGAAGTGTCACAATTTAACAAATACTTATGGCACACTTATCATC
AATTTATTGAAAAACATTGCTGAATCTGATCAAATTTGTTACAAACTTAATTTATGTGCA
TCAGACGAGAAAAACAACACAGGAATGGCTCGTTTTTACTAA
>g2782.t1 Gene=g2782 Length=893
MWKLNYFIVLLCVVSSSSSLANNVSQKYDAECSKGITFWCEHLMNAKKCNAVDFCIKTVW
ENHLVKIDSNPLCDDCKDWVKQARDLLERKETANAIIRTLAWSCALCPDTNGRTKCRQII
SNNVMEILKLLDSRMNPDAICSAIHFCNNREFTKIFEKAFELESQQKLQQKPRQKLLPFT
CGQCNHIGSVLGEKLANANHDDMLEGVLKVCGQMSSFSDACSNLVFKNFNDIYQQLPKLI
TKEKLCHSSCSQHYQNHEGIVDISPAFDNPNIPCELCEQLMLHLREVLIANTTEIEFKNI
LEGFCNQMPSIAQECVSLADQYYDSIYQYLVNGLDANKTCVMIGICSHKLDSYNAPAMPL
LSSEFFPVPEVDSIEVHIVTQDSSINLHKDGRLCTACEYTMNLVHIEMNKNGVQENILKK
VRAECKKLPAYVRQCEDLIDTFGDQIMNAIYQGTNPRLVCPLIKMCPPNLNFQYLQETAV
DEKPTCPFCLFAMQEVRDLVSSNASKTNVEKVLDQLCTHLSDKLKSQCIEFVNEYSSEVV
DMILANFTPQEACVFIKLCSQEKPKLKRFSMAGIFNENHSDEMIDFDDDDNNNSKLLDNP
QCDLCKAVIKLIEQRVIDIKSKDEIRRELENSCSHLKRFSKECKAFVDKYSDRIVDLVSK
ELAPENVCKELIFCVTEEKVDMQDYDVGLDIFAKSFSESAEVSEDLEVTGTSCIICEFIM
TKIDEELNDKQKDEDIKHIIKNVCSKMPSTVSKQCNQFIDYYFDMIIVLIETTKPSEMCK
ELKLCPKSNEELENQLVEIKGDIYTCAVCRGAVESLDSIIEDPQVDVKLENLEEKICEKF
AGKFKEKCHNLTNTYGTLIINLLKNIAESDQICYKLNLCASDEKNNTGMARFY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 37 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 67 | 149 | 2.5E-15 |
| 35 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 179 | 251 | 1.2E-6 |
| 39 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 269 | 348 | 1.6E-17 |
| 34 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 390 | 472 | 2.0E-15 |
| 33 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 480 | 561 | 6.4E-19 |
| 36 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 598 | 676 | 3.1E-17 |
| 40 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 709 | 788 | 2.4E-18 |
| 38 | g2782.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 802 | 881 | 8.9E-14 |
| 13 | g2782.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 8 | 355 | 3.2E-136 |
| 17 | g2782.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 8 | 355 | 3.2E-136 |
| 11 | g2782.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 379 | 567 | 3.2E-136 |
| 15 | g2782.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 379 | 567 | 3.2E-136 |
| 12 | g2782.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 592 | 799 | 3.2E-136 |
| 16 | g2782.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 592 | 799 | 3.2E-136 |
| 14 | g2782.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 790 | 883 | 3.2E-136 |
| 18 | g2782.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 790 | 883 | 3.2E-136 |
| 23 | g2782.t1 | PRINTS | PR01797 | Saposin signature | 25 | 49 | 4.2E-10 |
| 21 | g2782.t1 | PRINTS | PR01797 | Saposin signature | 54 | 74 | 4.2E-10 |
| 20 | g2782.t1 | PRINTS | PR01797 | Saposin signature | 137 | 155 | 4.2E-10 |
| 22 | g2782.t1 | PRINTS | PR01797 | Saposin signature | 515 | 537 | 4.2E-10 |
| 19 | g2782.t1 | PRINTS | PR01797 | Saposin signature | 537 | 560 | 4.2E-10 |
| 10 | g2782.t1 | Pfam | PF02199 | Saposin A-type domain | 31 | 61 | 3.3E-12 |
| 6 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 115 | 147 | 6.2E-6 |
| 9 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 313 | 346 | 1.6E-8 |
| 5 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 434 | 466 | 2.7E-6 |
| 1 | g2782.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 484 | 520 | 7.5E-9 |
| 8 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 527 | 559 | 1.4E-8 |
| 4 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 641 | 671 | 2.9E-8 |
| 2 | g2782.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 713 | 748 | 7.1E-7 |
| 3 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 753 | 785 | 4.8E-6 |
| 7 | g2782.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 847 | 879 | 9.2E-5 |
| 42 | g2782.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 43 | g2782.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 44 | g2782.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 14 | - |
| 45 | g2782.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 21 | - |
| 41 | g2782.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 893 | - |
| 56 | g2782.t1 | ProSiteProfiles | PS51110 | Saposin A-type domain profile. | 25 | 65 | 12.19 |
| 60 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 69 | 151 | 17.458 |
| 63 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 270 | 350 | 16.444 |
| 58 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 390 | 470 | 16.865 |
| 61 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 482 | 563 | 17.786 |
| 62 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 598 | 678 | 17.973 |
| 57 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 709 | 789 | 17.208 |
| 59 | g2782.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 802 | 883 | 15.586 |
| 47 | g2782.t1 | SMART | SM00162 | sapA_2 | 28 | 61 | 4.9E-5 |
| 51 | g2782.t1 | SMART | SM00741 | sapb_4 | 71 | 147 | 8.8E-13 |
| 48 | g2782.t1 | SMART | SM00741 | sapb_4 | 179 | 250 | 0.015 |
| 50 | g2782.t1 | SMART | SM00741 | sapb_4 | 272 | 346 | 1.8E-18 |
| 53 | g2782.t1 | SMART | SM00741 | sapb_4 | 392 | 466 | 8.6E-19 |
| 52 | g2782.t1 | SMART | SM00741 | sapb_4 | 484 | 559 | 3.7E-20 |
| 55 | g2782.t1 | SMART | SM00741 | sapb_4 | 600 | 674 | 1.2E-19 |
| 49 | g2782.t1 | SMART | SM00741 | sapb_4 | 711 | 785 | 1.2E-18 |
| 54 | g2782.t1 | SMART | SM00741 | sapb_4 | 804 | 879 | 1.6E-9 |
| 24 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 70 | 149 | 2.58E-13 |
| 25 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 179 | 247 | 1.41E-7 |
| 30 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 271 | 348 | 1.79E-16 |
| 27 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 431 | 520 | 2.5E-17 |
| 26 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 524 | 636 | 1.18E-15 |
| 28 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 710 | 787 | 3.25E-16 |
| 29 | g2782.t1 | SUPERFAMILY | SSF47862 | Saposin | 804 | 881 | 4.84E-13 |
| 32 | g2782.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 46 | g2782.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 31 | g2782.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 30 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006665 | sphingolipid metabolic process | BP |
| GO:0005764 | lysosome | CC |
| GO:0006629 | lipid metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
