General information for g2828.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dopamine receptor 2.
Parent gene
- The parent gene g2828 is located on chr_3: 20476024..20481674 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2828 | g2828.t1 | isoform | g2828.t1 | 20476024 | 20481674 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon1 | 20476024 | 20476302 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS1 | 20476024 | 20476302 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon2 | 20476462 | 20476500 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS2 | 20476462 | 20476500 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon3 | 20478683 | 20478767 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS3 | 20478683 | 20478767 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon4 | 20479438 | 20479498 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS4 | 20479438 | 20479498 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon5 | 20479605 | 20479715 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS5 | 20479605 | 20479715 |
| chr_3 | g2828 | g2828.t1 | exon | g2828.t1.exon6 | 20480510 | 20481674 |
| chr_3 | g2828 | g2828.t1 | cds | g2828.t1.CDS6 | 20480510 | 20481674 |
| chr_3 | g2828 | g2828.t1 | TSS | g2828.t1 | NA | NA |
| chr_3 | g2828 | g2828.t1 | TTS | g2828.t1 | NA | NA |
Sequences
>g2828.t1 Gene=g2828 Length=1740
ATGATAAATGCTACGGAGCGTACTTGGGATTACTCACAATTTATAAATGCCAGTTTAACA
CTTTACAGTAGCAATAATCATGTTAAATACGATAATAATATTAATGTTAGCAGCACTTCC
GACGAGTTGTTGCCGGTGCTGATATTGAATAATTCAAATTTATTAAATCTGTATTTGAAT
GTAAATTGTTGCCAAACATCAACAACTACTACTACAGTATCGACTACAACACTTGAACCA
AATTTCTCGCAGTACCTGCAAGAACTTCCGAGCGATCGAGTCAGCTTGCTAGTTTTTCTC
TTTCTATTTTCACTATCGACAGTTTTTGGCAATTCACTTGTCATTCTTGCTGTCATAAGA
GAACGTTATTTACACACGTCCACCAACTATTTCGTTACGAGCTTAGCGGTTGCTGATTGT
CTAGTTGGACTAGTGGTGATGCCATTTTCGGCACTCTATGAAGTGTTGCAAAATACGTGG
TTCTTTGGTGAAAATTGGTGTGACATTTGGCGATCGCTTGATGTTCTCTTTTCAACTGCT
TCCATTCTTAATCTCTGTGTTATATCACTCGACCGTTACTGGGCTATAACAGATCCTTTT
TCATATCCAATGAAGATGACACGTAATCGAGCTGTAGGGCTCATAGCTGCCGTGTGGGTG
TGCTCAAGTCTGATTAGTTTTCCTGCAATCTATTGGTGGCGTGCGGCAAGGGAAGGACCT
ATGCCGGCATTCAAATGCAATTTTACAGAACATCTAGGATATTTAGTGTTTTCTTCAACA
ATCTCCTTCTATCTTCCATTATTAGTTATGGTTTTTACATACTGTAGAATTTATAGAGCA
GCTGTCATTCAAACTAGATCACTTAAACTAGGCACAAAACAAGTGCTTATGGCATCGGGT
GAAATGCAATTGACCTTACGCATACATCGCGGTGGAACAACAAGAGAACGTGAACCTACT
CATCAGCCGCAGCATCGAGAAAGTCAGCCACAAGCTACGACAGCAAATTCAACGCCAGAA
GAACCCGATGAGGAGCCGTTATCTGCCTTACATAATAATGGAATTCGACACAATAGACAT
TTGGGTAAAAATTTTTCTCTTTCACGAAAACTGGCAAAATTTGCGAAAGAGAAAAAGGCT
GCAAAAACTCTAGGAATCGTTATGGGCGTATTCATAATTTGCTGGTTGCCTTTCTTTGTT
GTAAATTTACTCACCGGCCTTTGTGCGGATTGTATAGCACACGAAGAAATCGTGAGTGCT
GTTGTAACCTGGCTAGGATGGATAAATTCATCTATGAATCCAGTAATTTACGCATGCTGG
AGTCGTGATTTTAGAAGAGCTTTTGTGAGAATTTTATGTGTTTGCTGTCCAAGAAAAATT
AGAAGAAAATATCAACCGACAATGAGATCAAAGGCTTCACAGCAGTGTCACCTAGCAAAT
GTGGTTACTTCAACATCATCCAGCTGTAGTGTTTGCGATGGGAATCAACGCAGGACGGCA
CGTTTGAGGAATTGCTCAAATTGTACAAAAATAATCCACAATCTTCATCATGGTTGTTAT
CATCATCGTCATCATCGACGTAATCGTGTGATAAAACAAAACAAGCGATGCACATTCGCC
ATTACTACCACCGAAATTCCCAACACAAGCGAGCTTACTAGTGAGGAAAATCAGCACATG
TCATTCAAGAGTCATAGCTCAAATTCTAGCAATGCTTCATCCATTAAAATCATTGAGTAA
>g2828.t1 Gene=g2828 Length=579
MINATERTWDYSQFINASLTLYSSNNHVKYDNNINVSSTSDELLPVLILNNSNLLNLYLN
VNCCQTSTTTTTVSTTTLEPNFSQYLQELPSDRVSLLVFLFLFSLSTVFGNSLVILAVIR
ERYLHTSTNYFVTSLAVADCLVGLVVMPFSALYEVLQNTWFFGENWCDIWRSLDVLFSTA
SILNLCVISLDRYWAITDPFSYPMKMTRNRAVGLIAAVWVCSSLISFPAIYWWRAAREGP
MPAFKCNFTEHLGYLVFSSTISFYLPLLVMVFTYCRIYRAAVIQTRSLKLGTKQVLMASG
EMQLTLRIHRGGTTREREPTHQPQHRESQPQATTANSTPEEPDEEPLSALHNNGIRHNRH
LGKNFSLSRKLAKFAKEKKAAKTLGIVMGVFIICWLPFFVVNLLTGLCADCIAHEEIVSA
VVTWLGWINSSMNPVIYACWSRDFRRAFVRILCVCCPRKIRRKYQPTMRSKASQQCHLAN
VVTSTSSSCSVCDGNQRRTARLRNCSNCTKIIHNLHHGCYHHRHHRRNRVIKQNKRCTFA
ITTTEIPNTSELTSEENQHMSFKSHSSNSSNASSIKIIE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 33 | g2828.t1 | CDD | cd15067 | 7tmA_Dop1R2-like | 107 | 448 | 1.06121E-143 |
| 16 | g2828.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 105 | 451 | 3.6E-110 |
| 17 | g2828.t1 | Gene3D | G3DSA:1.20.120.10 | - | 286 | 368 | 3.6E-110 |
| 44 | g2828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 313 | 346 | - |
| 45 | g2828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 325 | 341 | - |
| 43 | g2828.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 548 | 579 | - |
| 2 | g2828.t1 | PANTHER | PTHR24248:SF176 | DOPAMINE RECEPTOR 2 | 84 | 470 | 5.6E-156 |
| 3 | g2828.t1 | PANTHER | PTHR24248 | ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR | 84 | 470 | 5.6E-156 |
| 8 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 95 | 119 | 8.1E-51 |
| 9 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 128 | 149 | 8.1E-51 |
| 13 | g2828.t1 | PRINTS | PR01103 | Adrenergic receptor signature | 148 | 159 | 1.8E-5 |
| 6 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 174 | 196 | 8.1E-51 |
| 12 | g2828.t1 | PRINTS | PR01103 | Adrenergic receptor signature | 202 | 210 | 1.8E-5 |
| 4 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 210 | 231 | 8.1E-51 |
| 10 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 255 | 278 | 8.1E-51 |
| 11 | g2828.t1 | PRINTS | PR01103 | Adrenergic receptor signature | 274 | 281 | 1.8E-5 |
| 7 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 380 | 404 | 8.1E-51 |
| 5 | g2828.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 419 | 445 | 8.1E-51 |
| 14 | g2828.t1 | PRINTS | PR01103 | Adrenergic receptor signature | 443 | 452 | 1.8E-5 |
| 1 | g2828.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 110 | 437 | 1.6E-82 |
| 25 | g2828.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 95 | - |
| 26 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 118 | - |
| 19 | g2828.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 119 | 129 | - |
| 31 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 130 | 149 | - |
| 24 | g2828.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 150 | 168 | - |
| 27 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 190 | - |
| 21 | g2828.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 191 | 210 | - |
| 30 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 211 | 233 | - |
| 22 | g2828.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 234 | 252 | - |
| 29 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 253 | 277 | - |
| 20 | g2828.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 278 | 382 | - |
| 28 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 383 | 405 | - |
| 23 | g2828.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 406 | 416 | - |
| 32 | g2828.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 417 | 440 | - |
| 18 | g2828.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 441 | 579 | - |
| 42 | g2828.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 180 | 196 | - |
| 46 | g2828.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 110 | 437 | 46.109 |
| 41 | g2828.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 101 | 452 | 1.2E-7 |
| 15 | g2828.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 77 | 471 | 1.1E-77 |
| 36 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 118 | - |
| 35 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 131 | 153 | - |
| 38 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 168 | 190 | - |
| 40 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 211 | 233 | - |
| 37 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 253 | 275 | - |
| 34 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 384 | 406 | - |
| 39 | g2828.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 421 | 440 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0004935 | adrenergic receptor activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04148 (dopamine receptor D1-like)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed