Gene loci information

Transcript annotation

  • This transcript has been annotated as Apyrase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g2835 g2835.t4 isoform g2835.t4 20499957 20502281
chr_3 g2835 g2835.t4 exon g2835.t4.exon1 20499957 20499959
chr_3 g2835 g2835.t4 exon g2835.t4.exon2 20500144 20500372
chr_3 g2835 g2835.t4 cds g2835.t4.CDS1 20500266 20500372
chr_3 g2835 g2835.t4 exon g2835.t4.exon3 20500516 20502281
chr_3 g2835 g2835.t4 cds g2835.t4.CDS2 20500516 20501365
chr_3 g2835 g2835.t4 TSS g2835.t4 20502370 20502370
chr_3 g2835 g2835.t4 TTS g2835.t4 NA NA

Sequences

>g2835.t4 Gene=g2835 Length=1998
ATGAAATTTATATTATGTTCGACAATTTTCATTCTTTATATTGGTTCTTGGATAATTTCA
AGTGAATCTGCTGCAGTTATTAAGAAAAATGAAAATCTTTTTCCACTTTCAATAATTCAT
GTTAATGATATTCATGCAAAGTAAGTGCACACACGTGTGACCTTTAAAATGTTAAATTGT
TATGATCATTACATACTTTTAAATTAGATTTGATGAAACTAATGTCGATAGTACAAGTTG
CAAGGAAAAATTAGGACAAGTATGCATAGGAGGATTTGCTCGTGTTATGACAATGGTTAA
GAAACTTAAAGCTGAAAGAGAGGCACAAAACAAAAATCCAATTTTTTTGAACATTGGTGA
TAATTTCGTTGGAACTTTATGGTATGAATTATTTGGATGGAATATTACATCAACTTTCTT
AAACATTCTTCCTGCAGACGCTACCACTTTAGGTTTGATATATATTTTAAAATAATTACT
TGATATTAAATAAATATTTTTTAATAGGAAATCATGAATTCGATAGAGGTGTACCTGAGG
TTGTGCATTTTCTTGAACATTTAATTTCGCCTGTAGTTGTTGCAAATTTAGATGATAAAG
ATGAACCAGATATGGCGAATAAATATAAAAAGTCAATTATTATCGAACGAGCAGGTAGAA
AGATTGGTTTAATTGGAGCACTTGTGGTTGCAACTTTGGAAATTTCGAGTCCAGGAAATT
TAAAAATTCTAGATGAAATTGAATCTGTCAAACAGGAAGCAGCAAGATTAAAAAGAGAAG
ATGACGTTGATATTATTATTGTTTTGTCACATTGTGGCCTTGTAATTGATCGTGAAATGG
CACAAAGTGGTGATGAAATAGATATTATTGTAGGTGGACATAGTCATTCTCTTCTTTATA
CAGGTAAATATTATCAATGAAAAACTTTTTCAAGTGGATGTTCCTTTATGCGTGTATTCT
TATCTTAGGTGATAATGTTCCCGGACCAGATGTTCCCGCAGATAAATATCCAATTGAATA
CAAACATGAAAGCGGACATAAAACAATTATCGTACAAGCTTCAGCTTATACAAAATATCT
CGGTGATTTAACAGTTTATTTTGATGAGAACGGAGAAATTGAGACATATGAAGGAAATAC
AATTTTCTTAGAAACAAGCATTGAACCTGATCCTGAAATTGTTGAACAATTAGCACCATG
GAAAGCTGCAGTCGATGCAATTGGACAAAGAAAAGTCGGTGATATCAAATCGATGCTGTA
TCAAAAAGATTGCGCTTTCGGAGAATGTAATATTGGCGATTTTGTGTGTGATGCTTTTAT
TAATTACATCGTGACACATGAAGATTATCAAGTTAATGATGGATGGGCATATGCAGCTAT
TGCAATTATTAATGCTGGTAGTATTCGCAATAATTTACCACCAGGTGAATTAATCTTTGA
TGATCTTTTCACTACAATTCCATTTATCAACACACTCGATGTTTTTGAGTTGCTTGGTCA
AGACTTGTTGGATGCACTTCAATTCTCTGGTAATGCTTATAGATATTACAACTTTTTGCA
ATTTTCTGGATTAAAAGTAACATACAACATTTCAATGCCAATGAATCAGCGAGTTATTTC
AGTAGATGTTTTATGTCGAGAATGTGAAATTCCAAAATATGAGAAATTGGATGTAACAAA
ATATTATCGTTTAATTATTTCAAGTTTTTTCGGTGATGGTGGAAATGGATATTTGATGTT
TAAAAACAAAAGAAATACCCGCACAATTGTAAAAATATGCATAAACTTAAACTCTTGTAT
GTTTCTTATTGACGTGTATTTTTTTTATTATTTTAACATCATCTGCTACGTCCACACATA
TAATTTAGGATAATTAGTCTTCTTTCACGAATTTGCCATTATAGAATGGCATAAATTGAA
GAATGAGTTGCCAATCTGTGATATAAAGCAAAGCCAAACTTCCAGCTGTTCCGAATGTTG
CTAAACTACCAACCCATT

>g2835.t4 Gene=g2835 Length=318
MKNFFKWMFLYACILILGDNVPGPDVPADKYPIEYKHESGHKTIIVQASAYTKYLGDLTV
YFDENGEIETYEGNTIFLETSIEPDPEIVEQLAPWKAAVDAIGQRKVGDIKSMLYQKDCA
FGECNIGDFVCDAFINYIVTHEDYQVNDGWAYAAIAIINAGSIRNNLPPGELIFDDLFTT
IPFINTLDVFELLGQDLLDALQFSGNAYRYYNFLQFSGLKVTYNISMPMNQRVISVDVLC
RECEIPKYEKLDVTKYYRLIISSFFGDGGNGYLMFKNKRNTRTIVKICINLNSCMFLIDV
YFFYYFNIICYVHTYNLG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g2835.t4 Gene3D G3DSA:3.60.21.10 - 14 103 7.2E-17
11 g2835.t4 Gene3D G3DSA:3.90.780.10 - 108 294 7.3E-43
2 g2835.t4 PANTHER PTHR11575 5’-NUCLEOTIDASE-RELATED 17 286 6.0E-86
3 g2835.t4 PANTHER PTHR11575:SF32 APYRASE-LIKE PROTEIN 17 286 6.0E-86
6 g2835.t4 PRINTS PR01607 Apyrase family signature 43 63 2.3E-12
4 g2835.t4 PRINTS PR01607 Apyrase family signature 171 194 2.3E-12
5 g2835.t4 PRINTS PR01607 Apyrase family signature 250 269 2.3E-12
1 g2835.t4 Pfam PF02872 5’-nucleotidase, C-terminal domain 107 276 2.2E-33
15 g2835.t4 Phobius SIGNAL_PEPTIDE Signal peptide region 1 28 -
16 g2835.t4 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 8 -
17 g2835.t4 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 9 17 -
20 g2835.t4 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 18 28 -
13 g2835.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 29 255 -
18 g2835.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 256 275 -
12 g2835.t4 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 276 286 -
19 g2835.t4 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 287 306 -
14 g2835.t4 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 307 318 -
8 g2835.t4 SUPERFAMILY SSF56300 Metallo-dependent phosphatases 24 100 1.8E-12
7 g2835.t4 SUPERFAMILY SSF55816 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain 105 281 3.92E-41
9 g2835.t4 SignalP_EUK SignalP-noTM SignalP-noTM 1 18 -
22 g2835.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 256 275 -
21 g2835.t4 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 287 309 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0016787 hydrolase activity MF
GO:0009166 nucleotide catabolic process BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values