General information for g2835.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Apyrase.
Parent gene
- The parent gene g2835 is located on chr_3: 20500421..20502281 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2835 | g2835.t5 | TTS | g2835.t5 | 20500351 | 20500351 |
| chr_3 | g2835 | g2835.t5 | isoform | g2835.t5 | 20500368 | 20501311 |
| chr_3 | g2835 | g2835.t5 | exon | g2835.t5.exon1 | 20500368 | 20501311 |
| chr_3 | g2835 | g2835.t5 | cds | g2835.t5.CDS1 | 20500421 | 20501029 |
| chr_3 | g2835 | g2835.t5 | TSS | g2835.t5 | NA | NA |
Sequences
>g2835.t5 Gene=g2835 Length=944
GATAATGTTCCCGGACCAGATGTTCCCGCAGATAAATATCCAATTGAATACAAACATGAA
AGCGGACATAAAACAATTATCGTACAAGCTTCAGCTTATACAAAATATCTCGGTGATTTA
ACAGTTTATTTTGATGAGAACGGAGAAATTGAGACATATGAAGGAAATACAATTTTCTTA
GAAACAAGCATTGAACCTGATCCTGAAATTGTTGAACAATTAGCACCATGGAAAGCTGCA
GTCGATGCAATTGGACAAAGAAAAGTCGGTGATATCAAATCGATGCTGTATCAAAAAGAT
TGCGCTTTCGGAGAATGTAATATTGGCGATTTTGTGTGTGATGCTTTTATTAATTACATC
GTGACACATGAAGATTATCAAGTTAATGATGGATGGGCATATGCAGCTATTGCAATTATT
AATGCTGGTAGTATTCGCAATAATTTACCACCAGGTGAATTAATCTTTGATGATCTTTTC
ACTACAATTCCATTTATCAACACACTCGATGTTTTTGAGTTGCTTGGTCAAGACTTGTTG
GATGCACTTCAATTCTCTGGTAATGCTTATAGATATTACAACTTTTTGCAATTTTCTGGA
TTAAAAGTAACATACAACATTTCAATGCCAATGAATCAGCGAGTTATTTCAGTAGATGTT
TTATGTCGAGAATGTGAAATTCCAAAATATGAGAAATTGGATGTAACAAAATATTATCGT
TTAATTATTTCAAGTTTTTTCGGTGATGGTGGAAATGGATATTTGATGTTTAAAAACAAA
AGAAATACCCGCACAACTAAAGAAAAAGATATTGAAATTATAGAGCAATATTTCAAAAAG
ATGAGTCCCGTATTGCAGAAAAAGGATGGAAGAATAAAAGTTTTAACTTGATCATGTAGT
CAAATTATCCTATTATAATCAAGAATAAAGATTTCTTACTTGTA
>g2835.t5 Gene=g2835 Length=202
MLYQKDCAFGECNIGDFVCDAFINYIVTHEDYQVNDGWAYAAIAIINAGSIRNNLPPGEL
IFDDLFTTIPFINTLDVFELLGQDLLDALQFSGNAYRYYNFLQFSGLKVTYNISMPMNQR
VISVDVLCRECEIPKYEKLDVTKYYRLIISSFFGDGGNGYLMFKNKRNTRTTKEKDIEII
EQYFKKMSPVLQKKDGRIKVLT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g2835.t5 | Gene3D | G3DSA:3.90.780.10 | - | 2 | 201 | 0.0e+00 |
| 2 | g2835.t5 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 2 | 200 | 0.0e+00 |
| 3 | g2835.t5 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 2 | 200 | 0.0e+00 |
| 4 | g2835.t5 | PRINTS | PR01607 | Apyrase family signature | 59 | 82 | 8.1e-06 |
| 5 | g2835.t5 | PRINTS | PR01607 | Apyrase family signature | 138 | 157 | 8.1e-06 |
| 1 | g2835.t5 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 5 | 164 | 0.0e+00 |
| 6 | g2835.t5 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 5 | 201 | 0.0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
