General information for g2850.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Phosphoenolpyruvate carboxykinase [GTP].
Parent gene
- The parent gene g2850 is located on chr_3: 20642851..20644603 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2850 | g2850.t2 | TSS | g2850.t2 | 20642288 | 20642288 |
| chr_3 | g2850 | g2850.t2 | isoform | g2850.t2 | 20642851 | 20644603 |
| chr_3 | g2850 | g2850.t2 | exon | g2850.t2.exon1 | 20642851 | 20644603 |
| chr_3 | g2850 | g2850.t2 | cds | g2850.t2.CDS1 | 20643092 | 20644603 |
| chr_3 | g2850 | g2850.t2 | TTS | g2850.t2 | 20644740 | 20644740 |
Sequences
>g2850.t2 Gene=g2850 Length=1753
TGGAAGTGAAATTGAAAATGAAATCTTTACCAAGTCTCTTTTGGAAGCTGGTACTTTAAT
TCCATTGCCAAAGTATCAAAATTGTTATTTGGCTCGTACTAATCCAGCTGATGTCGCTCG
TGTTGAGAGTAAAACTTTCATTTGCACTCCTCATCGTGAACAAGCTGTTTGCACACCACG
TCCAGGAATTCAAGGCACATTGGGACACTGGATTAGCCCAAAGGACTATGATGACGCTAT
TATGAATAGATTCCCAGGATGCATGAAGGGTCGCACAATGTATGTTGTTCCATTCTCGAT
GGGTCCAGTTGGTTCACCATTAAGCAAAGTCGGAATTGAAATCACTGATTCGCAATATGT
TGTCTGTTCAATGCGTATTATGACTCGTATGGGTTCACTCGTTATGGATGAAATTATCAA
GAATAATGTTGACTTTGTAAAATGCTTGCATTCAGTTGGAACACCTGAAAGTGGAAAAGT
TGCAATGGAATCATGGCCATGCGATCCAGATCGTGTGATCATTTTGCATAAACCAGAAAA
TAATGAAATTGTCTCATATGGTTCAGCTTATGGTGGCAATGCAATTTTGGGAAAGAAATG
CTTTGCTCTTCGTATTGGCAGCACTATTGCACAAAGAGAAGGCTGGTTGGCTGAACATAT
GTTAATTCTTGGAGTCACTTGCCCTAATGGTACAAAGAAATATATCGCTGCTGCTTTTCC
ATCAGCTTGCGGTAAAACAAATCTTGCTATGATGACACCAACATTACCCGGATACAAAAT
TGAGTGTGTTGGTGATGATATTGCATGGATGAAATTCGATGCTCAGGGACAATTACGTGC
AATTAATCCAGAAAATGGTTTCTTTGGTGTTGCTCCAGGCACATCATTCGAAACAAATCC
AAATGCTATGGAAACAGTCGTTAGAGATACAATTTTCACAAATGTCGCTTATACCTCAGA
TGGTGGTGTTTTCTGGGAAGGTCTTGAAAATACAATCAAACCAGGCGTCACAATCACTGA
TTGGCTTGGTAATCCATGGGAACTTGGTGTTAGCAAGACACCAGCAGCTCATCCAAATTC
ACGTTTCTGTTCACCAGCAAGTAACTGCCCAATCATTGATGATAAATGGGAAGATCCAGA
AGGTGTCCCAATCTCAGCTATTTTATTCGGTGGTAGACGACCAGAAGGTGTTCCACTCAT
TTATGAGGCTATGAATTGGCAGCATGGTGTGTTTATTGGTTCAGCAATGCGAAGTGAATC
AACTGCCGCTGCTGAACACAAAGGAAAGGTCATTATGCATGACCCAATGGCAATGAGACC
ATTCTTTGGTTATAATTTTGGCCATTACTTGAAACACTGGCTTGAAATGGAAGAGCAGTG
CAATAAACTCAATGGAACTATGCCAAAAGTGTTCCACGTCAACTGGTTCCGTAAGGACAA
GAATGGAAAGTTCTTATGGCCAGGATATGGCGAAAATTCACGTGTTCTTGATTGGATCTT
GAAGAGAATCGATGGTTTCGATTGCTATGATGAGACACCAATTGGCTATGTACCAACACC
AGAGTCATTAAGACTCGACAATCTCAAGTCACAAATCAACTTGAAAGAACTTTTCTCAAT
AGAACGCGAGTTTTGGTTGAAGGAAGCTGAGCAAATTCAAACTTATTATGATAATCAAGT
TGGAACTGATTTGCCAAAACAAATTCAACAACAATTGAATAATCTTAAGGCTAGAGTTGA
GAAGATGCAATAG
>g2850.t2 Gene=g2850 Length=503
MNRFPGCMKGRTMYVVPFSMGPVGSPLSKVGIEITDSQYVVCSMRIMTRMGSLVMDEIIK
NNVDFVKCLHSVGTPESGKVAMESWPCDPDRVIILHKPENNEIVSYGSAYGGNAILGKKC
FALRIGSTIAQREGWLAEHMLILGVTCPNGTKKYIAAAFPSACGKTNLAMMTPTLPGYKI
ECVGDDIAWMKFDAQGQLRAINPENGFFGVAPGTSFETNPNAMETVVRDTIFTNVAYTSD
GGVFWEGLENTIKPGVTITDWLGNPWELGVSKTPAAHPNSRFCSPASNCPIIDDKWEDPE
GVPISAILFGGRRPEGVPLIYEAMNWQHGVFIGSAMRSESTAAAEHKGKVIMHDPMAMRP
FFGYNFGHYLKHWLEMEEQCNKLNGTMPKVFHVNWFRKDKNGKFLWPGYGENSRVLDWIL
KRIDGFDCYDETPIGYVPTPESLRLDNLKSQINLKELFSIEREFWLKEAEQIQTYYDNQV
GTDLPKQIQQQLNNLKARVEKMQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g2850.t2 | CDD | cd00819 | PEPCK_GTP | 2 | 495 | 0.0 |
| 9 | g2850.t2 | Gene3D | G3DSA:3.40.449.10 | Phosphoenolpyruvate Carboxykinase | 2 | 133 | 6.0E-238 |
| 10 | g2850.t2 | Gene3D | G3DSA:3.90.228.20 | - | 135 | 496 | 6.0E-238 |
| 11 | g2850.t2 | Gene3D | G3DSA:2.170.8.10 | Phosphoenolpyruvate Carboxykinase | 207 | 289 | 6.0E-238 |
| 5 | g2850.t2 | Hamap | MF_00452 | Phosphoenolpyruvate carboxykinase [GTP] [pckG]. | 1 | 500 | 34.310005 |
| 3 | g2850.t2 | PANTHER | PTHR11561 | PHOSPHOENOLPYRUVATE CARBOXYKINASE | 2 | 502 | 3.5E-261 |
| 4 | g2850.t2 | PANTHER | PTHR11561:SF11 | PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP], MITOCHONDRIAL | 2 | 502 | 3.5E-261 |
| 1 | g2850.t2 | Pfam | PF17297 | Phosphoenolpyruvate carboxykinase N-terminal domain | 2 | 131 | 5.2E-61 |
| 2 | g2850.t2 | Pfam | PF00821 | Phosphoenolpyruvate carboxykinase C-terminal P-loop domain | 135 | 499 | 1.2E-166 |
| 8 | g2850.t2 | ProSitePatterns | PS00505 | Phosphoenolpyruvate carboxykinase (GTP) signature. | 159 | 167 | - |
| 7 | g2850.t2 | SUPERFAMILY | SSF68923 | PEP carboxykinase N-terminal domain | 2 | 134 | 3.09E-60 |
| 6 | g2850.t2 | SUPERFAMILY | SSF53795 | PEP carboxykinase-like | 135 | 501 | 1.49E-161 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004613 | phosphoenolpyruvate carboxykinase (GTP) activity | MF |
| GO:0006094 | gluconeogenesis | BP |
| GO:0005525 | GTP binding | MF |
| GO:0004611 | phosphoenolpyruvate carboxykinase activity | MF |
| GO:0017076 | purine nucleotide binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
