General information for g2853.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Major facilitator superfamily domain-containing protein 8.
Parent gene
- The parent gene g2853 is located on chr_3: 20661485..20664536 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2853 | g2853.t1 | TTS | g2853.t1 | 20659495 | 20659495 |
| chr_3 | g2853 | g2853.t1 | isoform | g2853.t1 | 20659902 | 20662293 |
| chr_3 | g2853 | g2853.t1 | exon | g2853.t1.exon1 | 20659902 | 20660226 |
| chr_3 | g2853 | g2853.t1 | cds | g2853.t1.CDS1 | 20659902 | 20660226 |
| chr_3 | g2853 | g2853.t1 | exon | g2853.t1.exon2 | 20660283 | 20660754 |
| chr_3 | g2853 | g2853.t1 | cds | g2853.t1.CDS2 | 20660283 | 20660754 |
| chr_3 | g2853 | g2853.t1 | exon | g2853.t1.exon3 | 20660814 | 20661036 |
| chr_3 | g2853 | g2853.t1 | cds | g2853.t1.CDS3 | 20660814 | 20661036 |
| chr_3 | g2853 | g2853.t1 | exon | g2853.t1.exon4 | 20661093 | 20661521 |
| chr_3 | g2853 | g2853.t1 | cds | g2853.t1.CDS4 | 20661093 | 20661521 |
| chr_3 | g2853 | g2853.t1 | exon | g2853.t1.exon5 | 20662084 | 20662293 |
| chr_3 | g2853 | g2853.t1 | cds | g2853.t1.CDS5 | 20662084 | 20662293 |
| chr_3 | g2853 | g2853.t1 | TSS | g2853.t1 | NA | NA |
Sequences
>g2853.t1 Gene=g2853 Length=1659
ATGAGTCAAGAGCAACAGCCTGTTTATAACAACACAACAACAACAGCAATTGAGAATGGC
GACAAAAAAGATAATAAACTCATTATTCTTGAGACGCAAGAAGAAAGGAGAGATCGAATG
TGGTCATTACGCATCGTTTATTTTACGATGTTCTTGGTTAGCTTGGCTTTTTCGGTTGTT
CTTACTGGCGTGTGGCCACTGTTGGATCGCCTCGATCCACATGCAGGAAAAGAATTCATG
GGACTTATTGTTGCCGCTAATCCTTTAGGTCAAATGCTTTTTAGTCCAATTTTTGGTTAT
TGGTCAAACAAAATGAAATCTATTCGACTTCCGCTTATTGCATCGATGGCAATTTTTTGC
ATTGCAAGCGGAGTTTATTCTGTATTAGACATTTTCCCAACAAGCACCGTGAAATGGTGG
ATGTTTTTTGCTCGATTTTTTGTTGGTGTTTCATCAGCTAACATTGCTGTTTGCCGTTCA
TACCTCTCAGCTGCCACAACTCTCAAAGAGAGAACTGACTCAGTATCGATGATGAGTTTA
GCTCAGGTGCTAGGATTCGTATTTGGTCCAGCATTACAAGCTGCTGTTACACCACTCGGT
TCTGATGGTTTTATTTTTCAAGGATTAGTTATAAATATGTACACAGCAGCAGGATGGATC
AACGTTTTACTTGGTGCCATCAATTTAGTTTTATTCTTCCCATATTTCTTCAAAGATCGT
CGAGTAGCAGCCAAAGAACAAATGATTATCCAAGGAAAACAAACTGAAAAAGAAACTTAT
AAATCGGTTAAAATTGATTACACAACTGCATGGACATTAATCCTCTCATTCTTTGTTATT
GTGTTCAATTTTGTGTTGCTTGAAACCATTGGATCTTCCCTAACAATGGATCAATTTGCA
TGGAGTAAAGGTGAAGCTTTGTATTATGTCGGTATACTTATGTCAGTTGGAGCATTTATC
GCTTGTCTAGCGTTTTGTCTCATTTCTCCATTAACAAAGCGCTTCAAAGAAGCTGACTTG
CTCATTTGGGGAGGATTTTTCTTCATGGTCATTGGCCGACTTGTCTTCATTCCTTATCGT
GGTGATATACCAAAATTGGCAGTTGATCGTGAATATAAAATGGAAAATGGTACAATTGCA
TTTTATGATGAGGATGATCCAATCGTTTTGGGTTGTCCTGTAAACAGTCAACCGTGGTGT
GCAACAACTTTACAATTACAATTTCCTGAATTCTTATTTGGATTTGTAATTTCCACAATT
GGCTATCCCATCGGTGTCACATTAATCCAAACAATTTTCTCGAAAGTTCTTGGCTCAAGA
CCTCAAGGAACTTGGATGGGATTATTCACAGGAGCTGGTTGTGTGTCTAGAATTTTCGGA
CCATTAGCTGTTGGTTCACTTTACACTCGTTATGGTACAACATGGACTTTTACAATCACT
TGTCTGATGATGTTCTTGCCTATGATTTGGCTTTATTTACTTCGTGATCGTTTAGCTATT
GAAGAAGTTGACATAAAAACGATTGAAATGAGAAATCTCAATGGAAATTCTACCAAAATA
CAAAATGGCACAAATGGTTTAATTAAAGATAAAACTGTGATCATTAATGAGAATGAAACT
AATGATGAACAGGAGAAATTTATTTCAAATGGAAGATAA
>g2853.t1 Gene=g2853 Length=552
MSQEQQPVYNNTTTTAIENGDKKDNKLIILETQEERRDRMWSLRIVYFTMFLVSLAFSVV
LTGVWPLLDRLDPHAGKEFMGLIVAANPLGQMLFSPIFGYWSNKMKSIRLPLIASMAIFC
IASGVYSVLDIFPTSTVKWWMFFARFFVGVSSANIAVCRSYLSAATTLKERTDSVSMMSL
AQVLGFVFGPALQAAVTPLGSDGFIFQGLVINMYTAAGWINVLLGAINLVLFFPYFFKDR
RVAAKEQMIIQGKQTEKETYKSVKIDYTTAWTLILSFFVIVFNFVLLETIGSSLTMDQFA
WSKGEALYYVGILMSVGAFIACLAFCLISPLTKRFKEADLLIWGGFFFMVIGRLVFIPYR
GDIPKLAVDREYKMENGTIAFYDEDDPIVLGCPVNSQPWCATTLQLQFPEFLFGFVISTI
GYPIGVTLIQTIFSKVLGSRPQGTWMGLFTGAGCVSRIFGPLAVGSLYTRYGTTWTFTIT
CLMMFLPMIWLYLLRDRLAIEEVDIKTIEMRNLNGNSTKIQNGTNGLIKDKTVIINENET
NDEQEKFISNGR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 44 | g2853.t1 | CDD | cd17326 | MFS_MFSD8 | 43 | 498 | 8.05385E-117 |
| 3 | g2853.t1 | PANTHER | PTHR23510:SF3 | MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8 | 31 | 499 | 3.9E-146 |
| 4 | g2853.t1 | PANTHER | PTHR23510 | INNER MEMBRANE TRANSPORT PROTEIN YAJR | 31 | 499 | 3.9E-146 |
| 2 | g2853.t1 | Pfam | PF07690 | Major Facilitator Superfamily | 47 | 352 | 1.4E-27 |
| 1 | g2853.t1 | Pfam | PF07690 | Major Facilitator Superfamily | 407 | 497 | 2.5E-10 |
| 19 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 44 | - |
| 35 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 45 | 67 | - |
| 28 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 68 | 78 | - |
| 34 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 79 | 101 | - |
| 23 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 102 | 107 | - |
| 36 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 108 | 129 | - |
| 26 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 130 | 140 | - |
| 40 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 141 | 162 | - |
| 22 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 163 | 173 | - |
| 32 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 174 | 196 | - |
| 30 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 197 | 215 | - |
| 38 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 216 | 237 | - |
| 21 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 238 | 266 | - |
| 41 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 267 | 286 | - |
| 31 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 287 | 305 | - |
| 43 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 306 | 328 | - |
| 20 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 329 | 339 | - |
| 39 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 340 | 359 | - |
| 29 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 360 | 410 | - |
| 42 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 411 | 433 | - |
| 25 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 434 | 444 | - |
| 33 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 445 | 468 | - |
| 27 | g2853.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 469 | 473 | - |
| 37 | g2853.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 474 | 494 | - |
| 24 | g2853.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 495 | 552 | - |
| 18 | g2853.t1 | ProSiteProfiles | PS50850 | Major facilitator superfamily (MFS) profile. | 42 | 499 | 12.356 |
| 17 | g2853.t1 | SUPERFAMILY | SSF103473 | MFS general substrate transporter | 36 | 498 | 2.35E-42 |
| 15 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 43 | 65 | - |
| 8 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 80 | 102 | - |
| 12 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 109 | 131 | - |
| 11 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 141 | 163 | - |
| 6 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 183 | 205 | - |
| 10 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 215 | 237 | - |
| 5 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 269 | 291 | - |
| 16 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 306 | 328 | - |
| 9 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 340 | 357 | - |
| 14 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 411 | 433 | - |
| 7 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 445 | 467 | - |
| 13 | g2853.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 472 | 494 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0055085 | transmembrane transport | BP |
| GO:0022857 | transmembrane transporter activity | MF |
KEGG
Orthology
- This transcript has been annotated with K12307 (MFS transporter, ceroid-lipofuscinosis neuronal protein 7)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
