General information for g2966.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-mannosidase 2.
Parent gene
- The parent gene g2966 is located on chr_3: 21619326..21620737 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2966 | g2966.t1 | TTS | g2966.t1 | 21616842 | 21616842 |
| chr_3 | g2966 | g2966.t1 | isoform | g2966.t1 | 21617074 | 21620737 |
| chr_3 | g2966 | g2966.t1 | exon | g2966.t1.exon1 | 21617074 | 21617582 |
| chr_3 | g2966 | g2966.t1 | cds | g2966.t1.CDS1 | 21617074 | 21617582 |
| chr_3 | g2966 | g2966.t1 | exon | g2966.t1.exon2 | 21617647 | 21619835 |
| chr_3 | g2966 | g2966.t1 | cds | g2966.t1.CDS2 | 21617647 | 21619835 |
| chr_3 | g2966 | g2966.t1 | exon | g2966.t1.exon3 | 21619892 | 21620153 |
| chr_3 | g2966 | g2966.t1 | cds | g2966.t1.CDS3 | 21619892 | 21620153 |
| chr_3 | g2966 | g2966.t1 | exon | g2966.t1.exon4 | 21620329 | 21620737 |
| chr_3 | g2966 | g2966.t1 | cds | g2966.t1.CDS4 | 21620329 | 21620737 |
| chr_3 | g2966 | g2966.t1 | TSS | g2966.t1 | 21620868 | 21620868 |
Sequences
>g2966.t1 Gene=g2966 Length=3369
ATGATCAGAATCAAAAAGAAATTCTTGCTAGCCATCTTTGTAGTTGTCTTTTTCGTATAT
CTTTACTTCTATCTCTATTCAAATGTTCCAAGTAATAGTGAAAAATTCATAGATACAGTT
CCTAAAACTATACCAAATTACAAGAAATACTCTAATGTGCATGATGTTGTTGAAAAACCA
CTTGATGATATAAATGATAATGACAACTTACTTGGGGAAGAAGATCCAGAACAGAGTAAT
TTAGGAAAAAAAGATTTAATAAATGATCAAATTCTACCGGAAAATAATAACGATGTAGAA
TCATCATGTAACTTAGATATCGATACGGTACCAAATTCTGATATTCAAATGTTAAATGTC
TACAATGAAATACCATTCGATAATTTAGACGGAGGAGTGTGGAAACAAGGATGGAATATC
GAATATGACATTCATCAGTGGAACAGAAATCACAAACTCAAAGTTTTTATTGTGCCGCAT
TCTCATAATGATCCAGGATGGATTAACACGTTTGATGAGTATTATGAGAGGCAAACTAAG
CAAATATTTTCAAATATGCTTCGACATTTGGATGAAAATGAAAATATGAAATTTATATGG
GCAGAAATTGTATATTTTTCAAGATGGTACGATAGTCTTATGACATCTGAATCAAAAGAT
AAAGTTAAACGATTAATAAAGAATAAGCAACTTGAGTTTGTCACGGGTGGTTGGGTAATG
ACAGATGAAGCAGCATCACATTGGTATTCGATTCTCCAATCTTTAACTGAGGGCCAAGTG
TGGCTTAAAAAAACATTCAATATCAGCAACATTAAAAGTTCTTGGGCAATTGATCCTTTT
GGGCATTCTTCTGTTATGCCATATATTTTTAAAGAAGCTGGTTTTGAAAACATGTTAATT
CAAAGAACACATTATTCTGTCAAAAAATATTTGGCACAAAGAAAAGAATTAGAATTCCGA
TGGAGACAACTTTGGGATACAAAAGGAACAACTGATCTCTTTACTCACATGATGCCATTC
TATTCATATGATGTTCCTCATACATGTGGTCCCGATCCGAAAATTTGTTGTCAGTTTGAT
TTCAAAAGACTTCCTGGTTATGGACTAAGCTGCCCATGGAAAGTTCCGCCGGTTCCTATC
ACTGACGATAATGTGGCAAAAAGAGCAGAAATGATAGTTGATCAATGGAGGAAAAAGTCT
GTTTTGTATAAAACTCGGTCAGTGTTGATACCGTTGGGTGATGATTTTCGTTACACTCAA
TCAATGGAATGGGAAGCACAGCGTGTGAATTTTGAAAAAATATTTGAATATGTAAACAAT
GAACCCAGTTTTAACGTTGAAGCGAAATTTGCCACGCTGCAAGAATATTTCGATTCAGTG
CGTCATGAAAAAGGAACAAATCAATTTCCAAGTTTAACCGGAGACTTCTTTACATATGCC
GATCGTGACGACCATTATTGGTCAGGATATTTCACATCGAGACCATTTTACAAAAGAATT
GATCGTATTTTAATGAATTATCTAAGAACTGCTGAAATGCTTCATTCATGGACATCATGG
GAAAAATCGACGGGATTTGAGAAGCGTCTAGAGCAGGCAAGAAGAGCTCTTTCGATTTTT
CAACATCATGATGGTGTTACTGGTACAGCTAAAGATTATGTAATGGCAGACTATAATGAA
ATGATGACTGATGGCATAAAGAATAGCAAATTTGTTATTCAACAAGCTGTCTATAAATTA
TTAACAGAATCAAATATTTATCATCCCGACTATTCATTTACTTATTTCAATATTGATGAC
TCTCGAACTGCTGCAAGCGATGATTCATCTCGTTCTGTTATTATAATTGGAGACGAAATT
CCATATAAATATGTTGTTATACACAATTCACAGACATATTTGCGAAATGAAGTTGTGGAA
TTCTTTGTAGCTAAACCTTTCGTGTGTGTAACTGACGCTGATGGAAATTCTGTTGATGCG
CAAATAACACCATTTTTCAGTTGGCATAAAGGAATCTATCAAGGTCTGCCACAACCACAA
TTTTCTACTACAAAATATCGATTAATTTTTTCAGCAAGCGCACCAGCTTTAGGATTGTCA
GTATATAAATTGAATGCCAAGAACAAGAGAGACGAATGTCCTTCAACTACTTACTCGAAA
ATAACAATTTTTACTGATAATCAGTTTGCAATAAATTTAGACGAGTATCCCGAAAAAGTT
ACATTTGCTGAACCAAAAGAAACATCATTAAGAATAAATGAGAATTCGCCTGGAGCAAGT
TTCAACAAATATGGTATTCTTAAATCATTATCACAAGATAGCAAATCACCAGTTGTACCT
GTACATTTGGAATTTATGAGATATGGCACAAGAATGGCACATGGTCAAAGATCTGGTGCA
TATTTATTTTTACCAGATGGTCCAGCGCAAAAACTTGAAATTAAAGAAAATCCTACAGTC
GTTATTTCAAACGGTCAACTTGAGGCCTCTATTTCAACTGGTTTATCATTTGTCATTCAC
GAGAGTATTTTAAGGAGTGGGGGAGATGCTCTAGAAATAAGAAATTCAGTTGACATACGT
GAAACGAGCAATACCGAAATTATTATGCGATTAAGTACGAACATCAAAAATGAAAATGTT
TTTTTTACTGATTTGAATGGAATGCAATATATAAAGAGAAAAATATTGAAGAAAGTTCCG
CTTCAAGGGAATTACTATCCCATACCAACTGGAATTTTTATTGAAGACGATTTAATTCGT
TTGTCATTATTTACATCTACACCTCTTGGAGGTTCATCGCTGAATCCTGGCGAAATTGAA
ATAATGCAAGATAGACGATTGAATCAAGATGATGAACGAGGTTTGGGTCAAGGTGTTACT
GACAATAAACCGGTATTAAATATTTTCAAGTTAGTGCTTGAAAGTCGAGAATCTTGTAAA
AAGAATGATGAACAATATCCTATGGGTTTTCTTACGACCACTGCCTATATGGAACAAAAG
AAACTACTTCATCCATTTGAGAAATTAATATATTCTGAAAATGACTGGAATGGAGTGAAA
CATAGTTTCGGAAATGATCATGAAGAACAAGAGCCCGGATTTGAAATTGTAGTAATGCGA
ACATTACCTCACATACAAGCAAATGCAATAGGATTAGTTGTACATAGAACGAATTTTGAT
AAGTGCTCAGTCGAATCATCAATGAATGGAAATCAATTTAATATTAAAAGACTTCTGGGA
CTTGACGAAAATGAAGAGTCAAGAGAGATTTATCGTTCAACAGTGACACTTCTAGAGCCA
CATCAAAAAGTTAGCGATGAATCGATTCCAATGTGTGGAATGGATATTAAAGGATTCATT
TTAAAATAA
>g2966.t1 Gene=g2966 Length=1122
MIRIKKKFLLAIFVVVFFVYLYFYLYSNVPSNSEKFIDTVPKTIPNYKKYSNVHDVVEKP
LDDINDNDNLLGEEDPEQSNLGKKDLINDQILPENNNDVESSCNLDIDTVPNSDIQMLNV
YNEIPFDNLDGGVWKQGWNIEYDIHQWNRNHKLKVFIVPHSHNDPGWINTFDEYYERQTK
QIFSNMLRHLDENENMKFIWAEIVYFSRWYDSLMTSESKDKVKRLIKNKQLEFVTGGWVM
TDEAASHWYSILQSLTEGQVWLKKTFNISNIKSSWAIDPFGHSSVMPYIFKEAGFENMLI
QRTHYSVKKYLAQRKELEFRWRQLWDTKGTTDLFTHMMPFYSYDVPHTCGPDPKICCQFD
FKRLPGYGLSCPWKVPPVPITDDNVAKRAEMIVDQWRKKSVLYKTRSVLIPLGDDFRYTQ
SMEWEAQRVNFEKIFEYVNNEPSFNVEAKFATLQEYFDSVRHEKGTNQFPSLTGDFFTYA
DRDDHYWSGYFTSRPFYKRIDRILMNYLRTAEMLHSWTSWEKSTGFEKRLEQARRALSIF
QHHDGVTGTAKDYVMADYNEMMTDGIKNSKFVIQQAVYKLLTESNIYHPDYSFTYFNIDD
SRTAASDDSSRSVIIIGDEIPYKYVVIHNSQTYLRNEVVEFFVAKPFVCVTDADGNSVDA
QITPFFSWHKGIYQGLPQPQFSTTKYRLIFSASAPALGLSVYKLNAKNKRDECPSTTYSK
ITIFTDNQFAINLDEYPEKVTFAEPKETSLRINENSPGASFNKYGILKSLSQDSKSPVVP
VHLEFMRYGTRMAHGQRSGAYLFLPDGPAQKLEIKENPTVVISNGQLEASISTGLSFVIH
ESILRSGGDALEIRNSVDIRETSNTEIIMRLSTNIKNENVFFTDLNGMQYIKRKILKKVP
LQGNYYPIPTGIFIEDDLIRLSLFTSTPLGGSSLNPGEIEIMQDRRLNQDDERGLGQGVT
DNKPVLNIFKLVLESRESCKKNDEQYPMGFLTTTAYMEQKKLLHPFEKLIYSENDWNGVK
HSFGNDHEEQEPGFEIVVMRTLPHIQANAIGLVVHRTNFDKCSVESSMNGNQFNIKRLLG
LDENEESREIYRSTVTLLEPHQKVSDESIPMCGMDIKGFILK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g2966.t1 | CDD | cd10809 | GH38N_AMII_GMII_SfManIII_like | 152 | 491 | 0.0 |
| 10 | g2966.t1 | Gene3D | G3DSA:3.20.110.10 | Glycoside hydrolase 38 | 102 | 483 | 3.7E-164 |
| 12 | g2966.t1 | Gene3D | G3DSA:1.20.1270.50 | Families 57/38 glycoside transferase middle domain | 484 | 594 | 8.9E-40 |
| 11 | g2966.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 595 | 1015 | 1.9E-166 |
| 13 | g2966.t1 | Gene3D | G3DSA:2.70.98.30 | - | 722 | 1006 | 1.9E-166 |
| 5 | g2966.t1 | PANTHER | PTHR11607:SF57 | ALPHA-MANNOSIDASE 2X | 108 | 1119 | 5.2E-266 |
| 6 | g2966.t1 | PANTHER | PTHR11607 | ALPHA-MANNOSIDASE | 108 | 1119 | 5.2E-266 |
| 3 | g2966.t1 | Pfam | PF01074 | Glycosyl hydrolases family 38 N-terminal domain | 154 | 480 | 6.5E-99 |
| 2 | g2966.t1 | Pfam | PF09261 | Alpha mannosidase middle domain | 485 | 580 | 2.0E-24 |
| 1 | g2966.t1 | Pfam | PF07748 | Glycosyl hydrolases family 38 C-terminal domain | 761 | 953 | 3.7E-33 |
| 4 | g2966.t1 | Pfam | PF18230 | Glycosyl hydrolases family 38 C-terminal sub-domain | 1035 | 1120 | 1.0E-6 |
| 14 | g2966.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 16 | g2966.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
| 15 | g2966.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 27 | 1122 | - |
| 19 | g2966.t1 | SMART | SM00872 | Alpha_mann_mid_2 | 485 | 562 | 3.3E-30 |
| 7 | g2966.t1 | SUPERFAMILY | SSF88713 | Glycoside hydrolase/deacetylase | 107 | 482 | 2.11E-130 |
| 8 | g2966.t1 | SUPERFAMILY | SSF88688 | Families 57/38 glycoside transferase middle domain | 485 | 591 | 1.77E-30 |
| 9 | g2966.t1 | SUPERFAMILY | SSF74650 | Galactose mutarotase-like | 610 | 1121 | 3.77E-109 |
| 18 | g2966.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030246 | carbohydrate binding | MF |
| GO:0006013 | mannose metabolic process | BP |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0004559 | alpha-mannosidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01231 (alpha-mannosidase II)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
