General information for g2977.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glucose dehydrogenase.
Parent gene
- The parent gene g2977 is located on chr_3: 21688578..21693718 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2977 | g2977.t1 | isoform | g2977.t1 | 21688578 | 21693718 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon1 | 21688578 | 21688770 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS1 | 21688578 | 21688770 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon2 | 21688832 | 21688858 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS2 | 21688832 | 21688858 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon3 | 21688925 | 21689086 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS3 | 21688925 | 21689086 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon4 | 21689171 | 21689201 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS4 | 21689171 | 21689201 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon5 | 21689265 | 21689362 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS5 | 21689265 | 21689362 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon6 | 21689424 | 21690571 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS6 | 21689424 | 21690571 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon7 | 21690637 | 21690802 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS7 | 21690637 | 21690802 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon8 | 21691515 | 21691926 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS8 | 21691515 | 21691926 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon9 | 21691985 | 21692047 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS9 | 21691985 | 21692047 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon10 | 21692107 | 21692141 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS10 | 21692107 | 21692141 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon11 | 21692206 | 21693221 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS11 | 21692206 | 21693221 |
| chr_3 | g2977 | g2977.t1 | exon | g2977.t1.exon12 | 21693284 | 21693718 |
| chr_3 | g2977 | g2977.t1 | cds | g2977.t1.CDS12 | 21693284 | 21693718 |
| chr_3 | g2977 | g2977.t1 | TSS | g2977.t1 | NA | NA |
| chr_3 | g2977 | g2977.t1 | TTS | g2977.t1 | NA | NA |
Sequences
>g2977.t1 Gene=g2977 Length=3786
ATGAGCGCAATAGCGGGTGGTGTAGCAGCATTGAATGGAGTGGCAGCAGCAGCAGCAAGC
AATGCAGGCTGGTTCATTCCTATGCTTGTGGCTGCAATCGCATACTTTCAATATGAAGAA
TTTGTCGATCCTGAATCGAAACCAATTAACATTGCTTCAGAGAATTTATTGAATGAATAT
GACTTTATAGTTGTTGGTAGTGGTAGTGCAGGTGCAGTTGTTGCTGCACGTTTGTCTGAA
GTGGAAAATTGGAATGTTTTGTTACTTGAAGCTGGTGGCGATGAAACAGAAATATCAGAC
GTACCGTTAATGGCTGGCTATCTTCAATTGAGCAAATTAGACTGGCAATATAAATCAGAG
CCATCTGGAACGTTCTGCTTAGCCATGAATGGAGGAAGATGTAATTGGCCTAGAGGAAAA
GTACTAGGTGGCAGTAGTGTGTTAAATTACATGCTTTACTTGCGTGGCAATAAAAAAGAT
TATGATCGATGGGAAGAAGCTGGCAATGCAGGCTGGTCATACAAAGATGTTTTGTATTAT
TTTAAGAAATCAGAAGATAACACGAATCCATATCTAGCAAATACACCGTATCACGGAACA
GGAGGATATTTGACAATTGGAGAAGCTCCATATCACACTCCATTAGCTGCTGCATTTGTT
GAAGCTGGCGTTGAAATGGGTTACGATAATCGTGATCTGAATGGCGAAAAACAGACGGGT
TTTATGATAGCACAAGGTACAATTAGACGTGGAAGTCGATGCTCAACAGCGAAAGCATTT
TTAAGACCAGCACGTTTAAGACCGAATCTTCACATTGCAATGTTTTCTCACGTGACAAAA
GTCCTCATTGATCCAATTACTAAAATTGCTTATGGCATTGAATTTGTACGTGACCGAAAA
ATGCACTACGTTCGTGCAAAAAAAGAAGTCATTTTATCAGCTGGTTCAATAAACACTCCA
CAACTCTTGATGTTGAGTGGAATTGGACCAAAAGCGGAATTGGCAAAGCATAGAATCCCT
TTGATTCGCGATGCTGCTGTTGGACAAAATCTTCAAGATCATGTGGCTCTTGCTGGTCTT
ACATTTTTAGTAAATCAAGAAGTTTCGGTTGTTGAAAATCGATTTCATACAATGTCAACT
GTAATGGAATATGCAGTATTAGGACAAGGACCATTAACTGTTATGGGAGGTGTTGAAGGT
TTGGCTTTTGTCAACACAAAATATGTCAACGCTTCGGACGATTTTCCTGACATTGAATTT
CACTTTGTGTCAGGATCAACAAATTCAGATGGTGGAAGTCAATTACGAAAAATTCATGGA
CTGACTGATTCATTTTACAATAAAGTTTTCGCACCAATTAATAATCGAGATGTGTGGAGC
ATTATTCCTATGTTATTAAGACCAAAAAGCGTTGGTCAAATTAAATTAAGAAGCAACAAT
CCATTTGATTATCCGTATATTTATCCCAACTATTTCAAAGACGATTTTGACATGAAAACA
TTAATTGAAGGAGTGAAAATTGCTGTTGCGCTTTCGAGAACTGTGTCAATGCAACGATAT
AAATCAACACTGAGTCCACTTAAATTCCCTGGTTGTGCACATATACAAGTTTATACAGAT
TTATATTGGGAATGTATGATACGTCACTACACAGCCACAATTTATCACCCTGTGGGCACA
GCAAAAATGGGACCATACTGGGACAAAGCTGCTGTTGTTGATCCACAACTGAGAGTTTAT
GGTGTGCGAGGATTGAGAGTAATCGATGGTAGTATAATGCCTAATCAAGTTAGTGCGAAT
ACTAATGCACCTATTATAATGATCGAAAGTCTGATGGCATCGTTAGCTGGAACATATTCA
GCTCTGAGAATTTTATTTTCTTATGGGCCAGGATTTTTGATGCTTATGTTACTTGATTCA
TCAATTTGGATGCAAAGGCCTGATATAGTGGATTATAAACATCGTGTTAGAGATATTCCA
ACGCAACATATTTACAACATTTACGACTTTATTGTCATTGGTGGTGGATCAGCTGGTGCC
GTTGTTGCTGCACGTTTATCAGAAATTGCAGAATGGAATGTATTATTATTAGAAGCTGGA
CCAGATGAAACGTTTCTCTCAGATGTTCCACTGATATTTCCTACGCTTCAGCAATCAGAC
CTCGACTGGAAATTTCGAACTGAAACCTCAGATAGATTTTGCCTTGCAATGAATAATGGC
AGGTGCAATTGGCCACGAGGCAAAGTATTGGGTGGAAGCAGTGTACTTAACGCTATGCTT
TATGTTCGTGGTAATAAACGTGATTACGATGAGTGGGAGCAGCTTGGTAATACAGGCTGG
GGTTGGGAAAATGTTCTTCATTATTTTAAAAAACTTGAAACTGTTAAAGTTCCATATTTA
AAAAATCATCCATTGCGTGGAACAAATGGACCATTGCATGCAGAACATTTTCGATATGTG
TCGCCATTGGCAGATATTTATCTCGAAGCAGCAGCTGAAATGAATCTTTTGAATGCAGAA
AATGATTACAATGGTCGTTCACAAACAGGATTTGCAAGGTCACAAGGTTCTTTACGTGAT
GGTTTGAGAATGTCAACTGCAAAAGCCTATCTTCGACCTATCAGACATAGATCAAATATC
CATGTCTCATTAAAATCACACGTAGAAAAAATTTTAATAAATCCTCAAACAAAGGAAGCT
TATGGAGTTCAATTTATGAAAGACGATCGAAAGTATGTTGTATTTGCAGCAAAAGAAGTA
ATTTTATCAGCTGGTGCAATTCAATCACCTCAACTTTTAATGCTTTCTGGCGTTGGTCCA
AAACAACATTTGGAATCGCATAATATTCCGGTTATTCATGATAGTCCTGGAGTTGGAGAA
AATTTACAAGATCACATAGCATCTGGTGGTGGAAATTATTTAATCACAAATCCTGTTTCA
AATGACACATTGAGTATTGTAATGCCGAAAATTTTACATGTTGATACTGTTAGAAAATTT
TCTTTTGATCAACGCGGTGCTTTATATGCAATGCCAGCTTGTGAAATTATGGGATTTGTT
AATACAAAATATCAAGATCCGAGTGAAGATCGACCTGATGTGCAATTTTTCTTAGCTTCA
TTTGCTGACTCAGCCGATGGTGGATTATTTGGAAAGCGTGCTTCTGGTATATCGGATGAT
TATTATGCTGAAGTTTATGAACGAATATTATTTCGAGATGCAGTCATGATTCTTCCATTG
CTATTAAGACCAAGAAGTCGAGGAAAAATTTTATTACGTGACGCAAATTCACACTCTCAT
CCGCTTATTTATCCCAATTATTTTGACGATCCACAAGATATTAAAGTGATGATTGAAGGC
TCAAAAATTGGTTACAAAATCATGCAAACAAGAGTTTTTCGTGCACTGAATGCCACACTC
AACCCAAATACTGTGCCTGCGTGTGAAAAATATGAAAAGCTCTCAGATGATTTTTGGGGC
TGTTTAGCACGACATTATACACAAACAATTTATCATCCGAGTGGCACTGCAAAAATGGGA
CCTTCTTCTGATAAAATGGCAGTTGTCGACTCAACATTACAAGTATATGGTGTGCGAAAA
TTGCGTGTTGTCGATTGCAGTATAATGCCCACAATTGTCTCAGGAAATACCAACATACCT
ACAGTGATGATTGGAGAGAAAGCAGCAGACATGATAAAAGCAAAATATCTTTCTTCAATG
CATACACACGGTCATCAACCGAATTACAGAAATATGGACTATTATAATGATATAAGAAGA
TTTTAA
>g2977.t1 Gene=g2977 Length=1261
MSAIAGGVAALNGVAAAAASNAGWFIPMLVAAIAYFQYEEFVDPESKPINIASENLLNEY
DFIVVGSGSAGAVVAARLSEVENWNVLLLEAGGDETEISDVPLMAGYLQLSKLDWQYKSE
PSGTFCLAMNGGRCNWPRGKVLGGSSVLNYMLYLRGNKKDYDRWEEAGNAGWSYKDVLYY
FKKSEDNTNPYLANTPYHGTGGYLTIGEAPYHTPLAAAFVEAGVEMGYDNRDLNGEKQTG
FMIAQGTIRRGSRCSTAKAFLRPARLRPNLHIAMFSHVTKVLIDPITKIAYGIEFVRDRK
MHYVRAKKEVILSAGSINTPQLLMLSGIGPKAELAKHRIPLIRDAAVGQNLQDHVALAGL
TFLVNQEVSVVENRFHTMSTVMEYAVLGQGPLTVMGGVEGLAFVNTKYVNASDDFPDIEF
HFVSGSTNSDGGSQLRKIHGLTDSFYNKVFAPINNRDVWSIIPMLLRPKSVGQIKLRSNN
PFDYPYIYPNYFKDDFDMKTLIEGVKIAVALSRTVSMQRYKSTLSPLKFPGCAHIQVYTD
LYWECMIRHYTATIYHPVGTAKMGPYWDKAAVVDPQLRVYGVRGLRVIDGSIMPNQVSAN
TNAPIIMIESLMASLAGTYSALRILFSYGPGFLMLMLLDSSIWMQRPDIVDYKHRVRDIP
TQHIYNIYDFIVIGGGSAGAVVAARLSEIAEWNVLLLEAGPDETFLSDVPLIFPTLQQSD
LDWKFRTETSDRFCLAMNNGRCNWPRGKVLGGSSVLNAMLYVRGNKRDYDEWEQLGNTGW
GWENVLHYFKKLETVKVPYLKNHPLRGTNGPLHAEHFRYVSPLADIYLEAAAEMNLLNAE
NDYNGRSQTGFARSQGSLRDGLRMSTAKAYLRPIRHRSNIHVSLKSHVEKILINPQTKEA
YGVQFMKDDRKYVVFAAKEVILSAGAIQSPQLLMLSGVGPKQHLESHNIPVIHDSPGVGE
NLQDHIASGGGNYLITNPVSNDTLSIVMPKILHVDTVRKFSFDQRGALYAMPACEIMGFV
NTKYQDPSEDRPDVQFFLASFADSADGGLFGKRASGISDDYYAEVYERILFRDAVMILPL
LLRPRSRGKILLRDANSHSHPLIYPNYFDDPQDIKVMIEGSKIGYKIMQTRVFRALNATL
NPNTVPACEKYEKLSDDFWGCLARHYTQTIYHPSGTAKMGPSSDKMAVVDSTLQVYGVRK
LRVVDCSIMPTIVSGNTNIPTVMIGEKAADMIKAKYLSSMHTHGHQPNYRNMDYYNDIRR
F
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g2977.t1 | Gene3D | G3DSA:3.50.50.60 | - | 60 | 613 | 2.9E-183 |
| 23 | g2977.t1 | Gene3D | G3DSA:3.30.560.10 | Glucose Oxidase | 99 | 555 | 2.9E-183 |
| 20 | g2977.t1 | Gene3D | G3DSA:3.50.50.60 | - | 668 | 1233 | 3.4E-185 |
| 22 | g2977.t1 | Gene3D | G3DSA:3.30.560.10 | Glucose Oxidase | 705 | 1171 | 3.4E-185 |
| 6 | g2977.t1 | PANTHER | PTHR11552:SF186 | AGAP003786-PA | 26 | 610 | 0.0 |
| 8 | g2977.t1 | PANTHER | PTHR11552 | GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE | 26 | 610 | 0.0 |
| 5 | g2977.t1 | PANTHER | PTHR11552:SF186 | AGAP003786-PA | 614 | 1247 | 0.0 |
| 7 | g2977.t1 | PANTHER | PTHR11552 | GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE | 614 | 1247 | 0.0 |
| 1 | g2977.t1 | Pfam | PF00732 | GMC oxidoreductase | 60 | 355 | 7.1E-85 |
| 3 | g2977.t1 | Pfam | PF05199 | GMC oxidoreductase | 468 | 608 | 7.3E-37 |
| 2 | g2977.t1 | Pfam | PF00732 | GMC oxidoreductase | 668 | 966 | 4.2E-83 |
| 4 | g2977.t1 | Pfam | PF05199 | GMC oxidoreductase | 1084 | 1228 | 3.0E-37 |
| 27 | g2977.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 28 | g2977.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 29 | g2977.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 32 | g2977.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 26 | g2977.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 624 | - |
| 30 | g2977.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 625 | 644 | - |
| 24 | g2977.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 645 | 664 | - |
| 31 | g2977.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 665 | 686 | - |
| 25 | g2977.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 687 | 1261 | - |
| 18 | g2977.t1 | ProSitePatterns | PS00623 | GMC oxidoreductases signature 1. | 139 | 162 | - |
| 16 | g2977.t1 | ProSitePatterns | PS00624 | GMC oxidoreductases signature 2. | 315 | 329 | - |
| 19 | g2977.t1 | ProSitePatterns | PS00623 | GMC oxidoreductases signature 1. | 747 | 770 | - |
| 17 | g2977.t1 | ProSitePatterns | PS00624 | GMC oxidoreductases signature 2. | 925 | 939 | - |
| 14 | g2977.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 58 | 608 | 6.85E-76 |
| 13 | g2977.t1 | SUPERFAMILY | SSF54373 | FAD-linked reductases, C-terminal domain | 395 | 560 | 4.12E-46 |
| 15 | g2977.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 662 | 1236 | 2.22E-84 |
| 12 | g2977.t1 | SUPERFAMILY | SSF54373 | FAD-linked reductases, C-terminal domain | 1009 | 1176 | 1.18E-38 |
| 10 | g2977.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 13 | 35 | - |
| 11 | g2977.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 621 | 643 | - |
| 9 | g2977.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 664 | 686 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
