General information for g2985.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Trifunctional enzyme subunit beta, mitochondrial.
Parent gene
- The parent gene g2985 is located on chr_3: 21742765..21743844 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2985 | g2985.t1 | TTS | g2985.t1 | 21741991 | 21741991 |
| chr_3 | g2985 | g2985.t1 | isoform | g2985.t1 | 21742098 | 21743844 |
| chr_3 | g2985 | g2985.t1 | exon | g2985.t1.exon1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t1 | cds | g2985.t1.CDS1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t1 | exon | g2985.t1.exon2 | 21742657 | 21743205 |
| chr_3 | g2985 | g2985.t1 | cds | g2985.t1.CDS2 | 21742657 | 21743205 |
| chr_3 | g2985 | g2985.t1 | exon | g2985.t1.exon3 | 21743261 | 21743483 |
| chr_3 | g2985 | g2985.t1 | cds | g2985.t1.CDS3 | 21743261 | 21743483 |
| chr_3 | g2985 | g2985.t1 | exon | g2985.t1.exon4 | 21743545 | 21743624 |
| chr_3 | g2985 | g2985.t1 | cds | g2985.t1.CDS4 | 21743545 | 21743624 |
| chr_3 | g2985 | g2985.t1 | exon | g2985.t1.exon5 | 21743796 | 21743844 |
| chr_3 | g2985 | g2985.t1 | cds | g2985.t1.CDS5 | 21743796 | 21743844 |
| chr_3 | g2985 | g2985.t1 | TSS | g2985.t1 | 21743936 | 21743936 |
Sequences
>g2985.t1 Gene=g2985 Length=1401
ATGTCTTTACTGCGTGTACCAATTACTTTAGCTGTAAATCAGCAACAAGCTATTCGCACT
TTAAGCACATCATCATATTTATCTCAAAAGAAACAATTTAAATCAAAATCGCCTCAAAAT
ATTGTTTTGGTTGATGGTGTTCGTACCCCTTTTCTTGTTTCTGGAACTGATTATTCAAAA
ATGATGCCTCATAATCTTGCAAGACACTCTCTTCTTAGTTTGTTACGCAAAACAAAAGTG
GATAAGGAATTGATTGACTATATTGTTTATGGAACTGTAATTCAAGAAGTTAAAACATCG
AATATTGCACGTGAAGCAGCATTATCAGCAGGATTTAGCAATTTTACTCCAGCACATACT
GTTACAATGGCATGTATAAGCAGCAATCAAGCAATCACAACTGCTATGGGTTTAATTGCT
ACTGGAACATATGAATCTATTGTTTGTGGTGGTGTAGAATTTATGAGTGACGTACCTATT
CGTCATAGTAGAAAAATGCGATCATTAATGTTAAAAGCCAACAAAGCTAAAACACTTGGT
CAACGTCTTTCGTTACTATCAACATTCCGACCAAGCTTTTTGATTCCTGAACTCCCAGCT
GTAGCTGAATTTAGCAGTGGTGAAACAATGGGACATTCTGCTGATCGTTTGGCATCAGCA
TTTTCTGTTTCTAGAAAAGAACAAGATGATTATGCACTTCGTTCTCATACATTAGCTAAG
CAGGCTCAAGAGAAAGGATATTTTACGGATTTAGTTCCATTCAAAGTAGATGGTGTAGAA
AAAACAATCAACGTGGACAATGGAATTCGTGTTTCCACACCCGAAGCTTTAGCAAAATTG
AAGCCTGCATTTGTTAAACCTTATGGCACGATTACTGCAGCAAATGCTTCATATTTGACT
GATGGAGCATCAGCATGCTTAATTATGACTGAAGAAAAAGCAAAAGCATTAGGATTAAAA
CCAAAAGCATATCTTCGTGATTTCATTTATGTTTCACAAGATCCAATTGATCAACTTTTG
CTTGGGCCAGCATACGGAATTCCCAAATTGCTTAGCAAAACAGGACTTACACTTAAAGAC
ATTGATGTATGGGAAATTCATGAAGCTTTCGCTGGTCAAATTTTAGCTAACCTAAAAGCC
TTAGATTCTGATTATTTTTGCAAAGATTATTTGAAACTTGGTCAAAAATATGGTACACCT
GACTTAAGCAAATGGAATACATGGGGTGGATCGCTTTCAATTGGACATCCTTTTGCAGCT
ACTGGTGTGAGATTATGTATGCATACATCAAATAGATTAGTTCGTGAAAATGGTCAATTG
GGTGTTGTTGCTGCTTGTGCAGCTGGTGGTCAAGGTGTTGCAATGTTGATCGAAAGACAT
CCTGATGCTCATGCTAATTAA
>g2985.t1 Gene=g2985 Length=466
MSLLRVPITLAVNQQQAIRTLSTSSYLSQKKQFKSKSPQNIVLVDGVRTPFLVSGTDYSK
MMPHNLARHSLLSLLRKTKVDKELIDYIVYGTVIQEVKTSNIAREAALSAGFSNFTPAHT
VTMACISSNQAITTAMGLIATGTYESIVCGGVEFMSDVPIRHSRKMRSLMLKANKAKTLG
QRLSLLSTFRPSFLIPELPAVAEFSSGETMGHSADRLASAFSVSRKEQDDYALRSHTLAK
QAQEKGYFTDLVPFKVDGVEKTINVDNGIRVSTPEALAKLKPAFVKPYGTITAANASYLT
DGASACLIMTEEKAKALGLKPKAYLRDFIYVSQDPIDQLLLGPAYGIPKLLSKTGLTLKD
IDVWEIHEAFAGQILANLKALDSDYFCKDYLKLGQKYGTPDLSKWNTWGGSLSIGHPFAA
TGVRLCMHTSNRLVRENGQLGVVAACAAGGQGVAMLIERHPDAHAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g2985.t1 | CDD | cd00751 | thiolase | 42 | 459 | 8.18085E-166 |
| 10 | g2985.t1 | Gene3D | G3DSA:3.40.47.10 | - | 31 | 459 | 5.1E-121 |
| 3 | g2985.t1 | PANTHER | PTHR18919:SF147 | TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL | 32 | 459 | 3.0E-203 |
| 4 | g2985.t1 | PANTHER | PTHR18919 | ACETYL-COA C-ACYLTRANSFERASE | 32 | 459 | 3.0E-203 |
| 2 | g2985.t1 | Pfam | PF00108 | Thiolase, N-terminal domain | 41 | 312 | 1.5E-77 |
| 1 | g2985.t1 | Pfam | PF02803 | Thiolase, C-terminal domain | 319 | 459 | 2.7E-43 |
| 7 | g2985.t1 | ProSitePatterns | PS00098 | Thiolases acyl-enzyme intermediate signature. | 121 | 139 | - |
| 9 | g2985.t1 | ProSitePatterns | PS00737 | Thiolases signature 2. | 406 | 422 | - |
| 8 | g2985.t1 | ProSitePatterns | PS00099 | Thiolases active site. | 441 | 454 | - |
| 5 | g2985.t1 | SUPERFAMILY | SSF53901 | Thiolase-like | 41 | 319 | 1.7E-54 |
| 6 | g2985.t1 | SUPERFAMILY | SSF53901 | Thiolase-like | 320 | 459 | 4.87E-32 |
| 11 | g2985.t1 | TIGRFAM | TIGR01930 | AcCoA-C-Actrans: acetyl-CoA C-acyltransferase | 44 | 458 | 7.3E-124 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016746 | acyltransferase activity | MF |
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
- This transcript has been annotated with K07509 (acetyl-CoA acyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
