General information for g2985.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Trifunctional enzyme subunit beta, mitochondrial.
Parent gene
- The parent gene g2985 is located on chr_3: 21742765..21743844 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2985 | g2985.t10 | TTS | g2985.t10 | 21741991 | 21741991 |
| chr_3 | g2985 | g2985.t10 | isoform | g2985.t10 | 21742098 | 21743844 |
| chr_3 | g2985 | g2985.t10 | exon | g2985.t10.exon1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t10 | cds | g2985.t10.CDS1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t10 | exon | g2985.t10.exon2 | 21742657 | 21743205 |
| chr_3 | g2985 | g2985.t10 | cds | g2985.t10.CDS2 | 21742657 | 21743191 |
| chr_3 | g2985 | g2985.t10 | exon | g2985.t10.exon3 | 21743261 | 21743394 |
| chr_3 | g2985 | g2985.t10 | exon | g2985.t10.exon4 | 21743545 | 21743624 |
| chr_3 | g2985 | g2985.t10 | exon | g2985.t10.exon5 | 21743796 | 21743844 |
| chr_3 | g2985 | g2985.t10 | TSS | g2985.t10 | 21743936 | 21743936 |
Sequences
>g2985.t10 Gene=g2985 Length=1312
ATGTCTTTACTGCGTGTACCAATTACTTTAGCTGTAAATCAGCAACAAGCTATTCGCACT
TTAAGCACATCATCATATTTATCTCAAAAGAAACAATTTAAATCAAAATCGCCTCAAAAT
ATTGTTTTGTTTGTTACGCAAAACAAAAGTGGATAAGGAATTGATTGACTATATTGTTTA
TGGAACTGTAATTCAAGAAGTTAAAACATCGAATATTGCACGTGAAGCAGCATTATCAGC
AGGATTTAGCAATTTTACTCCAGCACATACTGTTACAATGGCATGTATAAGCAGCAATCA
AGCAATCACAACTGCTATGGGTTTAATTGCTACTGGAACATATGAATCTATTGTTTGTGG
TGGTGTAGAATTTATGAGTGACGTACCTATTCGTCATAGTAGAAAAATGCGATCATTAAT
GTTAAAAGCCAACAAAGCTAAAACACTTGGTCAACGTCTTTCGTTACTATCAACATTCCG
ACCAAGCTTTTTGATTCCTGAACTCCCAGCTGTAGCTGAATTTAGCAGTGGTGAAACAAT
GGGACATTCTGCTGATCGTTTGGCATCAGCATTTTCTGTTTCTAGAAAAGAACAAGATGA
TTATGCACTTCGTTCTCATACATTAGCTAAGCAGGCTCAAGAGAAAGGATATTTTACGGA
TTTAGTTCCATTCAAAGTAGATGGTGTAGAAAAAACAATCAACGTGGACAATGGAATTCG
TGTTTCCACACCCGAAGCTTTAGCAAAATTGAAGCCTGCATTTGTTAAACCTTATGGCAC
GATTACTGCAGCAAATGCTTCATATTTGACTGATGGAGCATCAGCATGCTTAATTATGAC
TGAAGAAAAAGCAAAAGCATTAGGATTAAAACCAAAAGCATATCTTCGTGATTTCATTTA
TGTTTCACAAGATCCAATTGATCAACTTTTGCTTGGGCCAGCATACGGAATTCCCAAATT
GCTTAGCAAAACAGGACTTACACTTAAAGACATTGATGTATGGGAAATTCATGAAGCTTT
CGCTGGTCAAATTTTAGCTAACCTAAAAGCCTTAGATTCTGATTATTTTTGCAAAGATTA
TTTGAAACTTGGTCAAAAATATGGTACACCTGACTTAAGCAAATGGAATACATGGGGTGG
ATCGCTTTCAATTGGACATCCTTTTGCAGCTACTGGTGTGAGATTATGTATGCATACATC
AAATAGATTAGTTCGTGAAAATGGTCAATTGGGTGTTGTTGCTGCTTGTGCAGCTGGTGG
TCAAGGTGTTGCAATGTTGATCGAAAGACATCCTGATGCTCATGCTAATTAA
>g2985.t10 Gene=g2985 Length=344
MACISSNQAITTAMGLIATGTYESIVCGGVEFMSDVPIRHSRKMRSLMLKANKAKTLGQR
LSLLSTFRPSFLIPELPAVAEFSSGETMGHSADRLASAFSVSRKEQDDYALRSHTLAKQA
QEKGYFTDLVPFKVDGVEKTINVDNGIRVSTPEALAKLKPAFVKPYGTITAANASYLTDG
ASACLIMTEEKAKALGLKPKAYLRDFIYVSQDPIDQLLLGPAYGIPKLLSKTGLTLKDID
VWEIHEAFAGQILANLKALDSDYFCKDYLKLGQKYGTPDLSKWNTWGGSLSIGHPFAATG
VRLCMHTSNRLVRENGQLGVVAACAAGGQGVAMLIERHPDAHAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g2985.t10 | CDD | cd00751 | thiolase | 1 | 337 | 7.47841E-129 |
| 6 | g2985.t10 | Gene3D | G3DSA:3.40.47.10 | - | 1 | 337 | 2.0E-96 |
| 3 | g2985.t10 | PANTHER | PTHR18919:SF147 | TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL | 1 | 337 | 9.8E-158 |
| 4 | g2985.t10 | PANTHER | PTHR18919 | ACETYL-COA C-ACYLTRANSFERASE | 1 | 337 | 9.8E-158 |
| 7 | g2985.t10 | PIRSF | PIRSF000429 | Ac-CoA_Ac_transf | 1 | 339 | 3.5E-99 |
| 2 | g2985.t10 | Pfam | PF00108 | Thiolase, N-terminal domain | 2 | 190 | 4.6E-53 |
| 1 | g2985.t10 | Pfam | PF02803 | Thiolase, C-terminal domain | 197 | 337 | 1.4E-43 |
| 10 | g2985.t10 | ProSitePatterns | PS00737 | Thiolases signature 2. | 284 | 300 | - |
| 9 | g2985.t10 | ProSitePatterns | PS00099 | Thiolases active site. | 319 | 332 | - |
| 5 | g2985.t10 | SUPERFAMILY | SSF53901 | Thiolase-like | 2 | 338 | 4.68E-39 |
| 11 | g2985.t10 | TIGRFAM | TIGR01930 | AcCoA-C-Actrans: acetyl-CoA C-acyltransferase | 1 | 336 | 4.5E-99 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016746 | acyltransferase activity | MF |
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
