General information for g2985.t11
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Trifunctional enzyme subunit beta, mitochondrial.
Parent gene
- The parent gene g2985 is located on chr_3: 21742765..21743844 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2985 | g2985.t11 | TTS | g2985.t11 | 21741991 | 21741991 |
| chr_3 | g2985 | g2985.t11 | isoform | g2985.t11 | 21742098 | 21743844 |
| chr_3 | g2985 | g2985.t11 | exon | g2985.t11.exon1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t11 | cds | g2985.t11.CDS1 | 21742098 | 21742597 |
| chr_3 | g2985 | g2985.t11 | exon | g2985.t11.exon2 | 21742657 | 21743205 |
| chr_3 | g2985 | g2985.t11 | cds | g2985.t11.CDS2 | 21742657 | 21743205 |
| chr_3 | g2985 | g2985.t11 | exon | g2985.t11.exon3 | 21743261 | 21743483 |
| chr_3 | g2985 | g2985.t11 | cds | g2985.t11.CDS3 | 21743261 | 21743432 |
| chr_3 | g2985 | g2985.t11 | exon | g2985.t11.exon4 | 21743545 | 21743844 |
| chr_3 | g2985 | g2985.t11 | TSS | g2985.t11 | 21743936 | 21743936 |
Sequences
>g2985.t11 Gene=g2985 Length=1572
ATGTCTTTACTGCGTGTACCAATTACTTTAGCTGTAAATCAGCAACAAGGTAAGCACAAA
TTTATAAGTTTACATAATAAATTCCAACATTTTAATTACGAGTACAAAGTCCAACAAATT
TTGTGATGAAGTCAAAATCCAAAATAATTATTATCATGCATATAGAAAGATAAAAATTAC
AATCGCTTACTCATAATATTTTATTTCTATGCAATTTCAGCTATTCGCACTTTAAGCACA
TCATCATATTTATCTCAAAAGAAACAATTTAAATCAAAATCGCCTCAAAATATTGTTTTG
GTTGATGGTGTTCGTACCCCTTTTCTTGTTTCTGGAACTGATTATTCAAAAATGATGCCT
CATAATCTTGCAAGACACTCTCTTCTTAGTTTGTTACGCAAAACAAAAGTGGATAAGGAA
TTGATTGACTATATTGTTTATGGAACTGTAATTCAAGAAGTTAAAACATCGAATATTGCA
CGTGAAGCAGCATTATCAGCAGGATTTAGCAATTTTACTCCAGCACATACTGTTACAATG
GCATGTATAAGCAGCAATCAAGCAATCACAACTGCTATGGGTTTAATTGCTACTGGAACA
TATGAATCTATTGTTTGTGGTGGTGTAGAATTTATGAGTGACGTACCTATTCGTCATAGT
AGAAAAATGCGATCATTAATGTTAAAAGCCAACAAAGCTAAAACACTTGGTCAACGTCTT
TCGTTACTATCAACATTCCGACCAAGCTTTTTGATTCCTGAACTCCCAGCTGTAGCTGAA
TTTAGCAGTGGTGAAACAATGGGACATTCTGCTGATCGTTTGGCATCAGCATTTTCTGTT
TCTAGAAAAGAACAAGATGATTATGCACTTCGTTCTCATACATTAGCTAAGCAGGCTCAA
GAGAAAGGATATTTTACGGATTTAGTTCCATTCAAAGTAGATGGTGTAGAAAAAACAATC
AACGTGGACAATGGAATTCGTGTTTCCACACCCGAAGCTTTAGCAAAATTGAAGCCTGCA
TTTGTTAAACCTTATGGCACGATTACTGCAGCAAATGCTTCATATTTGACTGATGGAGCA
TCAGCATGCTTAATTATGACTGAAGAAAAAGCAAAAGCATTAGGATTAAAACCAAAAGCA
TATCTTCGTGATTTCATTTATGTTTCACAAGATCCAATTGATCAACTTTTGCTTGGGCCA
GCATACGGAATTCCCAAATTGCTTAGCAAAACAGGACTTACACTTAAAGACATTGATGTA
TGGGAAATTCATGAAGCTTTCGCTGGTCAAATTTTAGCTAACCTAAAAGCCTTAGATTCT
GATTATTTTTGCAAAGATTATTTGAAACTTGGTCAAAAATATGGTACACCTGACTTAAGC
AAATGGAATACATGGGGTGGATCGCTTTCAATTGGACATCCTTTTGCAGCTACTGGTGTG
AGATTATGTATGCATACATCAAATAGATTAGTTCGTGAAAATGGTCAATTGGGTGTTGTT
GCTGCTTGTGCAGCTGGTGGTCAAGGTGTTGCAATGTTGATCGAAAGACATCCTGATGCT
CATGCTAATTAA
>g2985.t11 Gene=g2985 Length=406
MMPHNLARHSLLSLLRKTKVDKELIDYIVYGTVIQEVKTSNIAREAALSAGFSNFTPAHT
VTMACISSNQAITTAMGLIATGTYESIVCGGVEFMSDVPIRHSRKMRSLMLKANKAKTLG
QRLSLLSTFRPSFLIPELPAVAEFSSGETMGHSADRLASAFSVSRKEQDDYALRSHTLAK
QAQEKGYFTDLVPFKVDGVEKTINVDNGIRVSTPEALAKLKPAFVKPYGTITAANASYLT
DGASACLIMTEEKAKALGLKPKAYLRDFIYVSQDPIDQLLLGPAYGIPKLLSKTGLTLKD
IDVWEIHEAFAGQILANLKALDSDYFCKDYLKLGQKYGTPDLSKWNTWGGSLSIGHPFAA
TGVRLCMHTSNRLVRENGQLGVVAACAAGGQGVAMLIERHPDAHAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g2985.t11 | CDD | cd00751 | thiolase | 1 | 399 | 8.9851E-156 |
| 7 | g2985.t11 | Gene3D | G3DSA:3.40.47.10 | - | 1 | 399 | 1.6E-113 |
| 3 | g2985.t11 | PANTHER | PTHR18919:SF147 | TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL | 1 | 399 | 1.5E-192 |
| 4 | g2985.t11 | PANTHER | PTHR18919 | ACETYL-COA C-ACYLTRANSFERASE | 1 | 399 | 1.5E-192 |
| 8 | g2985.t11 | PIRSF | PIRSF000429 | Ac-CoA_Ac_transf | 1 | 401 | 1.5E-115 |
| 2 | g2985.t11 | Pfam | PF00108 | Thiolase, N-terminal domain | 2 | 252 | 7.7E-72 |
| 1 | g2985.t11 | Pfam | PF02803 | Thiolase, C-terminal domain | 259 | 399 | 2.0E-43 |
| 10 | g2985.t11 | ProSitePatterns | PS00098 | Thiolases acyl-enzyme intermediate signature. | 61 | 79 | - |
| 12 | g2985.t11 | ProSitePatterns | PS00737 | Thiolases signature 2. | 346 | 362 | - |
| 11 | g2985.t11 | ProSitePatterns | PS00099 | Thiolases active site. | 381 | 394 | - |
| 5 | g2985.t11 | SUPERFAMILY | SSF53901 | Thiolase-like | 3 | 259 | 5.76E-51 |
| 6 | g2985.t11 | SUPERFAMILY | SSF53901 | Thiolase-like | 260 | 399 | 3.68E-32 |
| 13 | g2985.t11 | TIGRFAM | TIGR01930 | AcCoA-C-Actrans: acetyl-CoA C-acyltransferase | 2 | 398 | 2.5E-118 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016746 | acyltransferase activity | MF |
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
- This transcript has been annotated with K00632 (acetyl-CoA acyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
