General information for g2993.t22
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cathepsin B.
Parent gene
- The parent gene g2993 is located on chr_3: 21868580..21876208 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g2993 | g2993.t22 | TTS | g2993.t22 | 21867629 | 21867629 |
| chr_3 | g2993 | g2993.t22 | isoform | g2993.t22 | 21867833 | 21869132 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon1 | 21867833 | 21867921 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS1 | 21867833 | 21867921 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon2 | 21867986 | 21868355 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS2 | 21867986 | 21868355 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon3 | 21868412 | 21868479 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS3 | 21868412 | 21868479 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon4 | 21868542 | 21868580 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS4 | 21868542 | 21868580 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon5 | 21868755 | 21868992 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS5 | 21868755 | 21868992 |
| chr_3 | g2993 | g2993.t22 | exon | g2993.t22.exon6 | 21869064 | 21869132 |
| chr_3 | g2993 | g2993.t22 | cds | g2993.t22.CDS6 | 21869064 | 21869132 |
| chr_3 | g2993 | g2993.t22 | TSS | g2993.t22 | 21869220 | 21869220 |
Sequences
>g2993.t22 Gene=g2993 Length=873
ATGAAATATTTCATATTATTTGCTGCATTGACAGCAGTTTGCTTTGGAGCTGACATTTTT
TCTGATGAATTCATCAATGAAATTAATAAAAAAGCAACAACATGGAAGGCTGGCCATAAT
TTTCATCCAGATACTAATTTGAAATATATCAAGAATTTACTTGGTGTTCATTCCGATTCT
AAGCATTTTAAACTACCTGAGTTATTGCATGACTCACGGGACATAAAAGATTTACCTGAA
AATTTTGATGCTCGTGAACAATGGCCTGATTGTCCAACATTGAGAGAAATAAGAGATCAA
GGAAGTTCTTGTGGATTTGGTTGCAATGGTGGCTTTCCAGGATCAGCTTGGAGCTATTGG
GTTAGAAAAGGTATTGTTAGTGGTGGACCATACAATTCTTCAATTGGATGTCAGCCATAT
GAAATCGCTCCTTGTGAACATCATGTCAATGGAACTCGTATGCCATGCTCTGGAGAAGGA
CATACACCAAAATGCATGAATAAATGCTCGAATCCCGCTTATAAAGTTGATTTCAAAACA
GACAAACACTTTGGCAAATCAAGCTATTCAGTAAAGCGTAATGAAGATCAAATTCGTTTG
GAAATTTTCAAAAATGGTCCAGTTGAAGGTGCATTCACAGTCTATGAAGATTTCGTTCAA
TATAAATCTGGAGTTTATCAACATGTTACTGGTAAAGCACTTGGTGGCCATGCTATTAAA
ATTTTCGGATGGGGAGTTGAAAATGGTGTCAAATACTGGTTGATTGCTAATAGCTGGAAT
TCAGATTGGGGTGATAATGGAACCTTCAAAATATTGCGAGGAGAAGACCATGTCGGTATT
GAGAGTGAGATTAGCGCTGGTTTGCCAAAGTAA
>g2993.t22 Gene=g2993 Length=290
MKYFILFAALTAVCFGADIFSDEFINEINKKATTWKAGHNFHPDTNLKYIKNLLGVHSDS
KHFKLPELLHDSRDIKDLPENFDAREQWPDCPTLREIRDQGSSCGFGCNGGFPGSAWSYW
VRKGIVSGGPYNSSIGCQPYEIAPCEHHVNGTRMPCSGEGHTPKCMNKCSNPAYKVDFKT
DKHFGKSSYSVKRNEDQIRLEIFKNGPVEGAFTVYEDFVQYKSGVYQHVTGKALGGHAIK
IFGWGVENGVKYWLIANSWNSDWGDNGTFKILRGEDHVGIESEISAGLPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g2993.t22 | CDD | cd02620 | Peptidase_C1A_CathepsinB | 79 | 287 | 4.19204E-113 |
| 10 | g2993.t22 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 17 | 100 | 8.7E-12 |
| 9 | g2993.t22 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 101 | 290 | 8.0E-80 |
| 4 | g2993.t22 | PANTHER | PTHR12411:SF737 | CATHEPSIN B | 13 | 105 | 3.4E-77 |
| 6 | g2993.t22 | PANTHER | PTHR12411 | CYSTEINE PROTEASE FAMILY C1-RELATED | 13 | 105 | 3.4E-77 |
| 3 | g2993.t22 | PANTHER | PTHR12411:SF737 | CATHEPSIN B | 105 | 287 | 3.4E-77 |
| 5 | g2993.t22 | PANTHER | PTHR12411 | CYSTEINE PROTEASE FAMILY C1-RELATED | 105 | 287 | 3.4E-77 |
| 1 | g2993.t22 | Pfam | PF08127 | Peptidase family C1 propeptide | 20 | 59 | 7.6E-19 |
| 2 | g2993.t22 | Pfam | PF00112 | Papain family cysteine protease | 105 | 287 | 2.8E-39 |
| 12 | g2993.t22 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 13 | g2993.t22 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 14 | g2993.t22 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 11 | - |
| 15 | g2993.t22 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 11 | g2993.t22 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 290 | - |
| 17 | g2993.t22 | SMART | SM00645 | pept_c1 | 78 | 288 | 1.6E-54 |
| 7 | g2993.t22 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 21 | 289 | 5.42E-79 |
| 8 | g2993.t22 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008234 | cysteine-type peptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004197 | cysteine-type endopeptidase activity | MF |
| GO:0050790 | regulation of catalytic activity | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed