General information for g3016.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g3016 is located on chr_3: 22093782..22098280 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3016 | g3016.t1 | isoform | g3016.t1 | 22093782 | 22098280 |
| chr_3 | g3016 | g3016.t1 | exon | g3016.t1.exon1 | 22093782 | 22096507 |
| chr_3 | g3016 | g3016.t1 | cds | g3016.t1.CDS1 | 22093782 | 22096507 |
| chr_3 | g3016 | g3016.t1 | exon | g3016.t1.exon2 | 22096571 | 22098001 |
| chr_3 | g3016 | g3016.t1 | cds | g3016.t1.CDS2 | 22096571 | 22098001 |
| chr_3 | g3016 | g3016.t1 | exon | g3016.t1.exon3 | 22098055 | 22098280 |
| chr_3 | g3016 | g3016.t1 | cds | g3016.t1.CDS3 | 22098055 | 22098280 |
| chr_3 | g3016 | g3016.t1 | TSS | g3016.t1 | NA | NA |
| chr_3 | g3016 | g3016.t1 | TTS | g3016.t1 | NA | NA |
Sequences
>g3016.t1 Gene=g3016 Length=4383
ATGTTACATAAGCTAAAAAAATCAATTGAAAGCATTTTATTTTCCAATCTAAAAACAATT
GAAAACTTTTACGTGAGAATTTTTTTCAAACTGACGTGGATTATTGCAGTTAGTCTTTCA
TTATGTGGCCTTGTGTTTTATTCATATGAAGCATTTTTAAAATGGCATTCTACTCCTGTT
ATTTTAATTTCAATCAAGTCAATTCAATCGATAGACATTCCTTTTCCTGCAATAACAACT
TGCAGACCAGATTTGCCTGAACAGTTTTATTTAAATATGGATCGAAATGCATTTTTTAAT
AGGTTTCATAATAATTCATTTCAAAAAAATTTTACATCAGAGGAGCAAAATAAAATGGCT
TTAACTGTTCAAACTTGTTATCCACAATTTTCCGAAATAATCAAAAAGAAATATTTTATC
AATAGAACTGAAAAAGATATTGCTGAATTCTTAAGAAATGCAACATTTAATTCTGGATTT
ACAAGTTGTGAAATTAACAAGAGAAGACAAAAATGTTTAAAAATTCTTTTTCGTACTTTG
ACGGATTTTGGTCTTTGCTATACATTTAATATGCAAAATTATGAGTATATTTTCAGAGAA
GGAAAAATCAGTGAAAGTTCGTTTATTTACAATAGTTATCTTAATAAAAGTTCAAAAAAT
AATTTCTCAAATATTTGGTCTTTAAATAAAGGTTATGACTCAGATTTAAATCTATTTGCA
CCACCACTTCGAGCAAAAAAGGAACATGGTTTTGATGTAAGAACAGATATAGCATGTATA
AAAACAGAATATTGTGAAGCAACAGGAAGAACTTTTAAATACATCATTCATTTACCAAAT
GAAATTCCAACTTTATTTCATGATACTCATTATGTCGAAATAAATAAAATGGCTTATTTT
GAAGTAAAAGTAAAAATTAATAAAATTAATCCAAACATCTTCAATTATCCTCCTGAAATT
CGTCAATGTTATATTGAAGGAGAAAAAAGATTGAAATTTTTCAAAACTTATACCAAAAAT
CTCTGTGATTTTGAATGCTTGACAAATTACACATTGGAAGTTTGTGGCTGTGTGAAATTC
ACAATGCCAAGGATAAATGACACTAAAGTTTGTGATATAAATAAAGTTCAATGTTATGAC
AAAATTATAAGAACATGGCCAAATGTGTATGATGAACAGTTATTAGAATCTAAATGTAAT
TGCCTTTCACCATGTACTGATATTTCATATTCCTTTCGTGAGAGAAAAAGAAATGCTTTC
TATCATACAACGGAAGTAAGTAAATATTATACACATTTTCATGCTGGAATAAAAGAACAT
ACAGCTAAAGAATACACCAGTTATGTTGCTTATACGCTTCAAAATTTTATAGCTGACTGT
GGTGGTTTAATAGGACTTTTTTTGGGATTTTCTTTACTGTCAATTTATGAAATATTTTAT
GATTTTATTGCATTTGCATTAAAATCATTGTTAAGTTCTGCAAAAAGATTATTGGAAAAT
TCATTTCTTAAAATAATTAGTAAACTTTTATGGATAGTTGCAGTTTTGTTTTCAATCAGT
GGTTTCTTATTTTATGTTATCAATATTTACATAAAATGGAAAATTGATCCTGATATTTTA
ATATCAATAAGAAAAATTTCATCAAATGAAATTCCTTTTCCAGCAATAACAATTTGTTAT
CCTTTCTTTCCAAATCAATTACAAAATCTTCCTGCAAATCCACATACAATTTTTATTAAT
AAATCATCGAAAATTTGGTCAACAAGATATACACAACTTGAAAAAAATTATTTAGCTGTA
GCTGCACAAACTTGTTATCTTGAAGAAAGTCAAGCAATGAAAAAAGAATTTTTTGTTAGT
AGTAAAAATTCAAACATTGTAGATTTACTTTTAAAGTCAGATTATAGTTTTGGTTATGGT
GATTGTGAATTTCATCTGATTACTATTAAATGTAAAAATATATTAAGTCGTTTTTTAACT
GACTATGGCATTTGTGAGACATATAATTCCCAAGGTTTTAAAAATATTTTTAGCGAAGCT
GCATCAAATAAAGATTTTGAAAAGTTCAAAGAAAGAGAAATCACAAAATATCCTTACTAT
TACAGTCCAAATCATAAAATAAAAGTCAACGATAGTGAAGATGCCAATTGGACACTTAGT
GATGGATATAAGATTAAGGATGAAGAAGTTTTGCCTGTAAGGGCATTGTCAAAAAATGTA
GTTGGATTTTATATTGACAAAATAGAAAGTTTATATCAATGCTATCCAAATGATCGTGGA
TATACGATCTATTTTCATTTACCAAATGAAATTCTTACACCATTTCATAAAAATCGTTTC
GTAGAGCTCACCCATGAAAAACGTTTTGTAATTAATGCTATAAAAATATCTAATGAAAAT
ATGAAAAATTATAAGCCTGAAACTCGTCTTTGTTATTATGAAGGCGAAAAAGTATTAAAA
TTTTTCAAAACTTATACCAAAAATCTTTGTGATTTTGAATGCATGACTAATTTTACATTG
GAAGTTTGTGATTGTGTCAAGTTCAATATGCCGAGATCAAATGAAACTGAAGTTTGTGGT
GTTGATAAAATTAAATGTTATCACAATATTAAAAAATCATGGCCAAATATACATAATATT
GATGTTTCAGAAGCTTGCAAATGTTTAGAACCTTGTACTAATATCAAATATTCCATAACT
GTAAAAAGAGAAGCTTTTCGTGCTAACATACAAACATATTTTACAGTTAGTTTTGCAAGT
CAAACAATTGAAGAACATTCATATTATGTTTCTTATACACTTCAGAATTTTATTGCAGAT
AGTGGTGGATTAATTGGATTATTTTTAGGCTTTAGTTTGCTTTCGATTTTTGAATTCATC
TATAGTTTTTTTATTACTTTCAAAAAAAGATGGAAGTTTCAAATGAAAATTTCTGATGTC
AAAATTGATCAAAGTACTTTAGGAGAATCATTTTCGGGATATTTTCAAAGAATTTTAAAA
GTTTTTACAATTTTGATTTCAATTTGTAGTTTGATATTTTATATTAATCAAGCTTTTGAA
AAGTGGCATATTTCTCCAGACATTTCAATTTCAACCAGTAAAATTCAGTCAAAAGAAATT
CCATTTCCAGCCATCACAATATGCAGTACAATTTTTCCTAGTGAAGCAATTCATTTTATA
AACCCCGTTTATCTTATTAATTATCTTAATCTTAGAAATAAAACTCTTAATTTAAGCGAG
ACACATCAAAATTATTTAGCTGTTGCTATTCAAACCTGCATTCAAAATATTTTTCATAGT
CAAAAATTGATTAAAAATAGAACTGAAACAGATATAATAAAACTTCTTTTAAACTCAACT
TCAAAAGTTTTCTTCAGTCGTTGTCAATTAGGAGACAAAATTGAAAATTGCGAAAAATTT
CTTCAAACATCTTTAACAGATATTGGAGTTTGTTATACAACCAATATGCAAAATTATAAA
AGAATTTTAAACAATGGTTTTGATATTTTACAATCTTCAAATCATAATTTATCAAATGAA
ACTCAATGGACACTTGGAGATGGATATTCAACACAGAATGATGATATTTTTCCATTTCGT
TCAATAAAACAAAATGTCATAAATTTAGAAATATCAATCAAAGAGTTTTTTGAAAGGAAA
TTTCAATTCTGCATGAATGAAGGCTTTGGATTTAAACTATTTTTTCATATGCCTAATGAA
ATTCCAACAACTTTTCATAAAACTCATTTTATTAAGAACTATTATACAAAAGTGATAAAA
TTAAATGCAAAAATTTATAACATTGATAAAAGTTTACAAACTTATCCGCCTAATGCAAGA
AACTGCTACCTAGAAGGTGAAAAAAGATTAAAATTTTTCAAAACCTACACAAAAAAACTT
TGTGATTTTGAATGTTTAACAAACTACACATTGAAAGTTTGTGGCTGTATAAAATTTACA
ATGCCAAAGTCAAATGACACTAAAGTTTGTGATATAGACAAATTTAAATGTTATTATGAA
ATAACGATGTCATGGCCAAACTTTAAAATCAAAAATTATGAAACAGATGTGTCAAAAAAC
TGCGAATGTTTAGAATCATGTACTAATATTGAATACTATATAGATGTTGAACAAGAGTCA
CTTTATGGAGATCAAATAGATGGAAGATCACATTTGAAAATAAAGTTTGAAAAAGAAACT
ATTAAAATGAACACAAAATATGTCGCTTATACGATTCAGAACTTTTTAGCTGATTGTGGT
GGATTAATTGGAATTTTCTTGGGATTTTCTTTGCTGTCGATTTTTGAGTTAATTTGCAAT
TTCGTAGTTTACTGTTTGAATAAAATTCAAACCAAAATTAAACCAAATATTGCATGGAAA
TAG
>g3016.t1 Gene=g3016 Length=1460
MLHKLKKSIESILFSNLKTIENFYVRIFFKLTWIIAVSLSLCGLVFYSYEAFLKWHSTPV
ILISIKSIQSIDIPFPAITTCRPDLPEQFYLNMDRNAFFNRFHNNSFQKNFTSEEQNKMA
LTVQTCYPQFSEIIKKKYFINRTEKDIAEFLRNATFNSGFTSCEINKRRQKCLKILFRTL
TDFGLCYTFNMQNYEYIFREGKISESSFIYNSYLNKSSKNNFSNIWSLNKGYDSDLNLFA
PPLRAKKEHGFDVRTDIACIKTEYCEATGRTFKYIIHLPNEIPTLFHDTHYVEINKMAYF
EVKVKINKINPNIFNYPPEIRQCYIEGEKRLKFFKTYTKNLCDFECLTNYTLEVCGCVKF
TMPRINDTKVCDINKVQCYDKIIRTWPNVYDEQLLESKCNCLSPCTDISYSFRERKRNAF
YHTTEVSKYYTHFHAGIKEHTAKEYTSYVAYTLQNFIADCGGLIGLFLGFSLLSIYEIFY
DFIAFALKSLLSSAKRLLENSFLKIISKLLWIVAVLFSISGFLFYVINIYIKWKIDPDIL
ISIRKISSNEIPFPAITICYPFFPNQLQNLPANPHTIFINKSSKIWSTRYTQLEKNYLAV
AAQTCYLEESQAMKKEFFVSSKNSNIVDLLLKSDYSFGYGDCEFHLITIKCKNILSRFLT
DYGICETYNSQGFKNIFSEAASNKDFEKFKEREITKYPYYYSPNHKIKVNDSEDANWTLS
DGYKIKDEEVLPVRALSKNVVGFYIDKIESLYQCYPNDRGYTIYFHLPNEILTPFHKNRF
VELTHEKRFVINAIKISNENMKNYKPETRLCYYEGEKVLKFFKTYTKNLCDFECMTNFTL
EVCDCVKFNMPRSNETEVCGVDKIKCYHNIKKSWPNIHNIDVSEACKCLEPCTNIKYSIT
VKREAFRANIQTYFTVSFASQTIEEHSYYVSYTLQNFIADSGGLIGLFLGFSLLSIFEFI
YSFFITFKKRWKFQMKISDVKIDQSTLGESFSGYFQRILKVFTILISICSLIFYINQAFE
KWHISPDISISTSKIQSKEIPFPAITICSTIFPSEAIHFINPVYLINYLNLRNKTLNLSE
THQNYLAVAIQTCIQNIFHSQKLIKNRTETDIIKLLLNSTSKVFFSRCQLGDKIENCEKF
LQTSLTDIGVCYTTNMQNYKRILNNGFDILQSSNHNLSNETQWTLGDGYSTQNDDIFPFR
SIKQNVINLEISIKEFFERKFQFCMNEGFGFKLFFHMPNEIPTTFHKTHFIKNYYTKVIK
LNAKIYNIDKSLQTYPPNARNCYLEGEKRLKFFKTYTKKLCDFECLTNYTLKVCGCIKFT
MPKSNDTKVCDIDKFKCYYEITMSWPNFKIKNYETDVSKNCECLESCTNIEYYIDVEQES
LYGDQIDGRSHLKIKFEKETIKMNTKYVAYTIQNFLADCGGLIGIFLGFSLLSIFELICN
FVVYCLNKIQTKIKPNIAWK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 27 | g3016.t1 | Gene3D | G3DSA:1.10.287.770 | - | 31 | 479 | 3.2E-37 |
| 20 | g3016.t1 | Gene3D | G3DSA:2.60.470.10 | - | 60 | 449 | 3.2E-37 |
| 23 | g3016.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 84 | 271 | 3.2E-37 |
| 26 | g3016.t1 | Gene3D | G3DSA:1.10.287.820 | - | 315 | 405 | 3.2E-37 |
| 24 | g3016.t1 | Gene3D | G3DSA:1.10.287.820 | - | 810 | 892 | 3.2E-7 |
| 29 | g3016.t1 | Gene3D | G3DSA:1.10.287.770 | - | 912 | 970 | 2.0E-8 |
| 28 | g3016.t1 | Gene3D | G3DSA:1.10.287.770 | - | 1002 | 1438 | 4.5E-32 |
| 21 | g3016.t1 | Gene3D | G3DSA:2.60.470.10 | - | 1028 | 1408 | 4.5E-32 |
| 22 | g3016.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 1051 | 1230 | 4.5E-32 |
| 25 | g3016.t1 | Gene3D | G3DSA:1.10.287.820 | - | 1274 | 1367 | 4.5E-32 |
| 6 | g3016.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 24 | 485 | 2.3E-126 |
| 9 | g3016.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 24 | 485 | 2.3E-126 |
| 5 | g3016.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 500 | 985 | 2.3E-126 |
| 8 | g3016.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 500 | 985 | 2.3E-126 |
| 4 | g3016.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 997 | 1446 | 2.3E-126 |
| 7 | g3016.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 997 | 1446 | 2.3E-126 |
| 10 | g3016.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 1038 | 1054 | 6.5E-5 |
| 13 | g3016.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 1139 | 1155 | 6.5E-5 |
| 11 | g3016.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 1406 | 1420 | 6.5E-5 |
| 12 | g3016.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 1420 | 1436 | 6.5E-5 |
| 3 | g3016.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 29 | 480 | 5.3E-56 |
| 2 | g3016.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 500 | 961 | 9.4E-52 |
| 1 | g3016.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 997 | 1439 | 1.2E-51 |
| 31 | g3016.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 26 | - |
| 38 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 27 | 49 | - |
| 34 | g3016.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 50 | 462 | - |
| 42 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 463 | 487 | - |
| 32 | g3016.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 488 | 507 | - |
| 40 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 508 | 531 | - |
| 36 | g3016.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 532 | 943 | - |
| 39 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 944 | 967 | - |
| 30 | g3016.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 968 | 997 | - |
| 37 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 998 | 1015 | - |
| 35 | g3016.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1016 | 1421 | - |
| 41 | g3016.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1422 | 1446 | - |
| 33 | g3016.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1447 | 1460 | - |
| 16 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 27 | 49 | - |
| 15 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 464 | 486 | - |
| 18 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 509 | 531 | - |
| 19 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 943 | 965 | - |
| 14 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 998 | 1015 | - |
| 17 | g3016.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1422 | 1444 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed