General information for g305.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine protease snake.
Parent gene
- The parent gene g305 is located on chr_3: 2636456..2637794 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g305 | g305.t1 | TSS | g305.t1 | 2635059 | 2635059 |
| chr_3 | g305 | g305.t1 | isoform | g305.t1 | 2635100 | 2636456 |
| chr_3 | g305 | g305.t1 | exon | g305.t1.exon1 | 2635100 | 2635163 |
| chr_3 | g305 | g305.t1 | cds | g305.t1.CDS1 | 2635100 | 2635163 |
| chr_3 | g305 | g305.t1 | exon | g305.t1.exon2 | 2635225 | 2635431 |
| chr_3 | g305 | g305.t1 | cds | g305.t1.CDS2 | 2635225 | 2635431 |
| chr_3 | g305 | g305.t1 | exon | g305.t1.exon3 | 2635499 | 2635615 |
| chr_3 | g305 | g305.t1 | cds | g305.t1.CDS3 | 2635499 | 2635615 |
| chr_3 | g305 | g305.t1 | exon | g305.t1.exon4 | 2635683 | 2636240 |
| chr_3 | g305 | g305.t1 | cds | g305.t1.CDS4 | 2635683 | 2636240 |
| chr_3 | g305 | g305.t1 | exon | g305.t1.exon5 | 2636305 | 2636456 |
| chr_3 | g305 | g305.t1 | cds | g305.t1.CDS5 | 2636305 | 2636456 |
| chr_3 | g305 | g305.t1 | TTS | g305.t1 | 2636551 | 2636551 |
Sequences
>g305.t1 Gene=g305 Length=1098
ATGGCGATAAAATTATTGATAATATTCTTTCTTTTTCCGAACGTTATATATTCTGCTATT
AATGGAGATCAATGTGTTACAAACAAAGGTGTTAGGGGCATTTGTATGGGTTTTTACGAA
TGTCAGAGCGCAGTCAATGACTACAGATTGGGAATTTTTCCTCAATTGTGTCAAAATTCA
AACAAATATACAGTTTGCTGTCCAGGAAATAATAAAGTACCTTCAAATAATATTAATAGA
ATTAATTCGCAAACTCGACCGAGCAAGCAAAAGTGTGATGAATATTCAAAGATAGCCACG
AATGTCTCACTTTTTGGAGGTCTCTCATTAGGAACATTTCACACTGAAAAAGTTGAAACA
TATACATGCGAATTGAGTATTTCATTAATTGTGGGTGGTGAACCTGCTAAAGCGAATGAA
TTTCCTCATATGGCTGCATTAGGATGGCGTGGATTAGATAGATATGAATTTAATTGTGGA
GCATCATTAATATCTGACAGATATGTTCTGACTGCCGCTCATTGTGAAAAATGGAATGGT
AAATTACCTGATATAGTTCGTTTAGGTGATTTAGATTTAAGATCAAATTCTGATGGTGTG
CAAGAACAAGATTTTCCTGTCGAAGCTTTCATTCGTCATGAAAATTATGATTCAAGACAA
AAGAAGAATGACATTGCTGTTGTTCGTATGAGATATAATGTAAGAGCATGGCAAAAAAAT
ATAAGACCGGCATGTCTTTGGTCTCAACAAAATATAAATCAGAATCAAGTTATTGCTACA
GGATATGGAAGTACTGGATATGCAGAAGGAAATAGCGATAAACTTTTAAAAGTTAATCTA
TTTGTCTCTAACACCTATAGATGTGGAGAAATAAATATTGATAACACTCAAATTTGTGCA
GGTGATGAAAGAAATAATAGAGATACATGCCAAGGTGACAGCGGAGGTCCACTTCAAATC
ACTCTTCCAAATAATAAGTGCACATTTTATATTGTTGGAATCACAAGTTTTGGTGAAAAT
GTTTGTGGTTATAGCAATGCAGTTTATACACGAGTGAGTGCTTATATAGATTGGATAGAA
AGAATAGTTTGGGGATAA
>g305.t1 Gene=g305 Length=365
MAIKLLIIFFLFPNVIYSAINGDQCVTNKGVRGICMGFYECQSAVNDYRLGIFPQLCQNS
NKYTVCCPGNNKVPSNNINRINSQTRPSKQKCDEYSKIATNVSLFGGLSLGTFHTEKVET
YTCELSISLIVGGEPAKANEFPHMAALGWRGLDRYEFNCGASLISDRYVLTAAHCEKWNG
KLPDIVRLGDLDLRSNSDGVQEQDFPVEAFIRHENYDSRQKKNDIAVVRMRYNVRAWQKN
IRPACLWSQQNINQNQVIATGYGSTGYAEGNSDKLLKVNLFVSNTYRCGEINIDNTQICA
GDERNNRDTCQGDSGGPLQITLPNNKCTFYIVGITSFGENVCGYSNAVYTRVSAYIDWIE
RIVWG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g305.t1 | CDD | cd00190 | Tryp_SPc | 130 | 362 | 7.17883E-73 |
| 10 | g305.t1 | Gene3D | G3DSA:2.40.10.10 | - | 130 | 352 | 4.9E-64 |
| 9 | g305.t1 | Gene3D | G3DSA:2.40.10.10 | - | 142 | 360 | 4.9E-64 |
| 2 | g305.t1 | PANTHER | PTHR24260 | - | 27 | 364 | 4.7E-75 |
| 3 | g305.t1 | PANTHER | PTHR24260:SF91 | FI18411P1-RELATED | 27 | 364 | 4.7E-75 |
| 4 | g305.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 160 | 175 | 1.3E-12 |
| 5 | g305.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 220 | 234 | 1.3E-12 |
| 6 | g305.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 307 | 319 | 1.3E-12 |
| 1 | g305.t1 | Pfam | PF00089 | Trypsin | 130 | 359 | 1.5E-48 |
| 12 | g305.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 13 | g305.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 14 | g305.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 12 | - |
| 15 | g305.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 18 | - |
| 11 | g305.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 365 | - |
| 19 | g305.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 170 | 175 | - |
| 20 | g305.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 308 | 319 | - |
| 22 | g305.t1 | ProSiteProfiles | PS51888 | Clip domain profile. | 24 | 67 | 7.698 |
| 21 | g305.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 130 | 364 | 26.551 |
| 18 | g305.t1 | SMART | SM00680 | clip | 25 | 68 | 0.0034 |
| 17 | g305.t1 | SMART | SM00020 | trypsin_2 | 129 | 359 | 4.7E-71 |
| 7 | g305.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 120 | 364 | 8.51E-69 |
| 8 | g305.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
