General information for g305.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine protease snake.
Parent gene
- The parent gene g305 is located on chr_3: 2636456..2637794 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g305 | g305.t2 | TSS | g305.t2 | 2635059 | 2635059 |
| chr_3 | g305 | g305.t2 | isoform | g305.t2 | 2635100 | 2636456 |
| chr_3 | g305 | g305.t2 | exon | g305.t2.exon1 | 2635100 | 2635431 |
| chr_3 | g305 | g305.t2 | cds | g305.t2.CDS1 | 2635209 | 2635431 |
| chr_3 | g305 | g305.t2 | exon | g305.t2.exon2 | 2635499 | 2635615 |
| chr_3 | g305 | g305.t2 | cds | g305.t2.CDS2 | 2635499 | 2635615 |
| chr_3 | g305 | g305.t2 | exon | g305.t2.exon3 | 2635683 | 2636240 |
| chr_3 | g305 | g305.t2 | cds | g305.t2.CDS3 | 2635683 | 2636240 |
| chr_3 | g305 | g305.t2 | exon | g305.t2.exon4 | 2636305 | 2636456 |
| chr_3 | g305 | g305.t2 | cds | g305.t2.CDS4 | 2636305 | 2636456 |
| chr_3 | g305 | g305.t2 | TTS | g305.t2 | 2636551 | 2636551 |
Sequences
>g305.t2 Gene=g305 Length=1159
ATGGCGATAAAATTATTGATAATATTCTTTCTTTTTCCGAACGTTATATATTCTGCTATT
AATGGTAAAAATTAAAGAAATTATTTTAATGTGAATGCTAAAATATTTAATGATTAATTT
AAAAGGAGATCAATGTGTTACAAACAAAGGTGTTAGGGGCATTTGTATGGGTTTTTACGA
ATGTCAGAGCGCAGTCAATGACTACAGATTGGGAATTTTTCCTCAATTGTGTCAAAATTC
AAACAAATATACAGTTTGCTGTCCAGGAAATAATAAAGTACCTTCAAATAATATTAATAG
AATTAATTCGCAAACTCGACCGAGCAAGCAAAAGTGTGATGAATATTCAAAGATAGCCAC
GAATGTCTCACTTTTTGGAGGTCTCTCATTAGGAACATTTCACACTGAAAAAGTTGAAAC
ATATACATGCGAATTGAGTATTTCATTAATTGTGGGTGGTGAACCTGCTAAAGCGAATGA
ATTTCCTCATATGGCTGCATTAGGATGGCGTGGATTAGATAGATATGAATTTAATTGTGG
AGCATCATTAATATCTGACAGATATGTTCTGACTGCCGCTCATTGTGAAAAATGGAATGG
TAAATTACCTGATATAGTTCGTTTAGGTGATTTAGATTTAAGATCAAATTCTGATGGTGT
GCAAGAACAAGATTTTCCTGTCGAAGCTTTCATTCGTCATGAAAATTATGATTCAAGACA
AAAGAAGAATGACATTGCTGTTGTTCGTATGAGATATAATGTAAGAGCATGGCAAAAAAA
TATAAGACCGGCATGTCTTTGGTCTCAACAAAATATAAATCAGAATCAAGTTATTGCTAC
AGGATATGGAAGTACTGGATATGCAGAAGGAAATAGCGATAAACTTTTAAAAGTTAATCT
ATTTGTCTCTAACACCTATAGATGTGGAGAAATAAATATTGATAACACTCAAATTTGTGC
AGGTGATGAAAGAAATAATAGAGATACATGCCAAGGTGACAGCGGAGGTCCACTTCAAAT
CACTCTTCCAAATAATAAGTGCACATTTTATATTGTTGGAATCACAAGTTTTGGTGAAAA
TGTTTGTGGTTATAGCAATGCAGTTTATACACGAGTGAGTGCTTATATAGATTGGATAGA
AAGAATAGTTTGGGGATAA
>g305.t2 Gene=g305 Length=349
MINLKGDQCVTNKGVRGICMGFYECQSAVNDYRLGIFPQLCQNSNKYTVCCPGNNKVPSN
NINRINSQTRPSKQKCDEYSKIATNVSLFGGLSLGTFHTEKVETYTCELSISLIVGGEPA
KANEFPHMAALGWRGLDRYEFNCGASLISDRYVLTAAHCEKWNGKLPDIVRLGDLDLRSN
SDGVQEQDFPVEAFIRHENYDSRQKKNDIAVVRMRYNVRAWQKNIRPACLWSQQNINQNQ
VIATGYGSTGYAEGNSDKLLKVNLFVSNTYRCGEINIDNTQICAGDERNNRDTCQGDSGG
PLQITLPNNKCTFYIVGITSFGENVCGYSNAVYTRVSAYIDWIERIVWG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g305.t2 | CDD | cd00190 | Tryp_SPc | 114 | 346 | 6.7065E-73 |
| 9 | g305.t2 | Gene3D | G3DSA:2.40.10.10 | - | 114 | 336 | 4.3E-64 |
| 8 | g305.t2 | Gene3D | G3DSA:2.40.10.10 | - | 126 | 344 | 4.3E-64 |
| 2 | g305.t2 | PANTHER | PTHR24260 | - | 42 | 348 | 2.1E-74 |
| 3 | g305.t2 | PANTHER | PTHR24260:SF91 | FI18411P1-RELATED | 42 | 348 | 2.1E-74 |
| 4 | g305.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 144 | 159 | 1.2E-12 |
| 6 | g305.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 204 | 218 | 1.2E-12 |
| 5 | g305.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 291 | 303 | 1.2E-12 |
| 1 | g305.t2 | Pfam | PF00089 | Trypsin | 114 | 343 | 1.3E-48 |
| 11 | g305.t2 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 154 | 159 | - |
| 12 | g305.t2 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 292 | 303 | - |
| 16 | g305.t2 | ProSiteProfiles | PS51888 | Clip domain profile. | 8 | 51 | 7.698 |
| 15 | g305.t2 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 114 | 348 | 26.551 |
| 14 | g305.t2 | SMART | SM00680 | clip | 9 | 52 | 0.0034 |
| 13 | g305.t2 | SMART | SM00020 | trypsin_2 | 113 | 343 | 4.7E-71 |
| 7 | g305.t2 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 104 | 348 | 9.66E-69 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
