General information for g3114.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Chitotriosidase-1.
Parent gene
- The parent gene g3114 is located on chr_3: 22904068..22905601 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3114 | g3114.t1 | isoform | g3114.t1 | 22904068 | 22905601 |
| chr_3 | g3114 | g3114.t1 | exon | g3114.t1.exon1 | 22904068 | 22904318 |
| chr_3 | g3114 | g3114.t1 | cds | g3114.t1.CDS1 | 22904068 | 22904318 |
| chr_3 | g3114 | g3114.t1 | exon | g3114.t1.exon2 | 22904381 | 22905019 |
| chr_3 | g3114 | g3114.t1 | cds | g3114.t1.CDS2 | 22904381 | 22905019 |
| chr_3 | g3114 | g3114.t1 | exon | g3114.t1.exon3 | 22905071 | 22905265 |
| chr_3 | g3114 | g3114.t1 | cds | g3114.t1.CDS3 | 22905071 | 22905265 |
| chr_3 | g3114 | g3114.t1 | exon | g3114.t1.exon4 | 22905322 | 22905601 |
| chr_3 | g3114 | g3114.t1 | cds | g3114.t1.CDS4 | 22905322 | 22905601 |
| chr_3 | g3114 | g3114.t1 | TSS | g3114.t1 | NA | NA |
| chr_3 | g3114 | g3114.t1 | TTS | g3114.t1 | NA | NA |
Sequences
>g3114.t1 Gene=g3114 Length=1365
ATGAAGGTGGTGTTCATTGCATTACTTTCATTAATTTTTTTAGAAAACTTTTCAATAGCT
TTAAGTGAAAATGAAGATAATGAAATTGGTGACGATAAAAAAGTTGTCTGTTATTTTGGA
TCTTGGAGTTATTATCGATTGGGAAAGGCAAAATTTACTGTAGATGACATTGAACCTCAA
TTATGCACTCACATTATGTATACCTTCTTTGGAGTACATGACAATGGAACATATAAATCT
TTAGATCCCTATTTAGATTATTCTGAAAATGATGGTCAAGGGTATGTTTCGAAATTTATA
AAATTAAAGGAAAAAAATCCATCAGTAAAATTAATGATATCAATCGGTGGTTGGAGCATG
GGAGGAGAAATGTTTTCAAATATTGTTTCAAATTCTACACTTAGAAAAAATTTCATATTA
AGCGTTCTCGATTTATTGCAAAAATTTAATTTCGATGGTTTTGATTTTGATTGGGAATAT
CCAAAAGAAGATGAAAAAGAAAAATTTATAAAATTATTAAAAAATCTCAAAAATAAATTG
CACGCTGCTGGAAAGCTTTTGACGATTGCAGTTGGTGCAACATCAAGTAGAGCTCAAGAG
TCTTATGACATTCCAAAAATTGCGGCTGAAGTTGATTTTATTAATTTAATGACTTATGAT
TTACATGGTATTTGGGATAGAAAGACAGGAATTCATTCTCCTTTATATGCAAATGATCAA
TTGAGTGTTGATGATTGCGTAAAATATTGGTTAGCACAAGGATGTCCGAAACATAAGTTG
ATTATAGGCATTCCATTATATGGCAGATCATTTAGTCTTTCAAGCACTAAAAGTTATCGT
ATTGGTCAACCAATTTCAGCAGCTGGAAAAGCAGGAAATTTCTTAAAAAGTGCTGACACA
GCAGGAAATTTGCCATATAATGAAATTTGTTTTAATATAAATAATAAAAAGTGGATTAGA
GTTTTTGCTAAGACAGAGAAAACACCATATGCATATAAAAAAGATCAATGGGTTAGCTAT
GATGATTTACAATCAATAGATTTAAAATTAGATTATATTCTCTCAAAAAATCTTGGTGGT
GCTATGTTTTGGAGTATTGAAACTGATGATTTTGGTAATATTTGTGGTGATGGAAAATTT
CCACTTTTATCATTAGCTCATCGTAGATTAATTCTCAATGATGAATCAAGTGAAAATTTT
GAAAGTACAGAAAATAATTCTAGTGATGATGAAGAAGAATCAACAAGTGATGAATGCGAA
GAAAGCAGTGAAGAAGAAGAGGAAGAAGAAAATGATAGAAATTTTAATGTTCCATTTTTT
ATTCAAATTATACAAAAATATAAGCAGAGAGCAATAAAGTCATAA
>g3114.t1 Gene=g3114 Length=454
MKVVFIALLSLIFLENFSIALSENEDNEIGDDKKVVCYFGSWSYYRLGKAKFTVDDIEPQ
LCTHIMYTFFGVHDNGTYKSLDPYLDYSENDGQGYVSKFIKLKEKNPSVKLMISIGGWSM
GGEMFSNIVSNSTLRKNFILSVLDLLQKFNFDGFDFDWEYPKEDEKEKFIKLLKNLKNKL
HAAGKLLTIAVGATSSRAQESYDIPKIAAEVDFINLMTYDLHGIWDRKTGIHSPLYANDQ
LSVDDCVKYWLAQGCPKHKLIIGIPLYGRSFSLSSTKSYRIGQPISAAGKAGNFLKSADT
AGNLPYNEICFNINNKKWIRVFAKTEKTPYAYKKDQWVSYDDLQSIDLKLDYILSKNLGG
AMFWSIETDDFGNICGDGKFPLLSLAHRRLILNDESSENFESTENNSSDDEEESTSDECE
ESSEEEEEEENDRNFNVPFFIQIIQKYKQRAIKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g3114.t1 | Coils | Coil | Coil | 412 | 432 | - |
| 8 | g3114.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 35 | 388 | 5.3E-124 |
| 9 | g3114.t1 | Gene3D | G3DSA:3.10.50.10 | - | 266 | 340 | 5.3E-124 |
| 20 | g3114.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 396 | 436 | - |
| 19 | g3114.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 404 | 432 | - |
| 2 | g3114.t1 | PANTHER | PTHR11177 | CHITINASE | 25 | 412 | 1.9E-116 |
| 3 | g3114.t1 | PANTHER | PTHR11177:SF360 | CHITINASE 12-RELATED | 25 | 412 | 1.9E-116 |
| 1 | g3114.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 34 | 369 | 1.7E-90 |
| 12 | g3114.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 13 | g3114.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 14 | g3114.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 15 | g3114.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 22 | - |
| 11 | g3114.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 454 | - |
| 17 | g3114.t1 | ProSitePatterns | PS01095 | Chitinases family 18 active site. | 151 | 159 | - |
| 18 | g3114.t1 | SMART | SM00636 | 2g34 | 33 | 369 | 8.4E-126 |
| 5 | g3114.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 33 | 389 | 7.03E-96 |
| 4 | g3114.t1 | SUPERFAMILY | SSF54556 | Chitinase insertion domain | 267 | 340 | 9.31E-15 |
| 7 | g3114.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 16 | g3114.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 6 | g3114.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0008061 | chitin binding | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01183 (chitinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed