General information for g3127.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mitochondrial potassium channel ATP-binding subunit.
Parent gene
- The parent gene g3127 is located on chr_3: 23077737..23080012 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3127 | g3127.t1 | TTS | g3127.t1 | 23077651 | 23077651 |
| chr_3 | g3127 | g3127.t1 | isoform | g3127.t1 | 23077737 | 23080012 |
| chr_3 | g3127 | g3127.t1 | exon | g3127.t1.exon1 | 23077737 | 23079323 |
| chr_3 | g3127 | g3127.t1 | cds | g3127.t1.CDS1 | 23077737 | 23079323 |
| chr_3 | g3127 | g3127.t1 | exon | g3127.t1.exon2 | 23079545 | 23080012 |
| chr_3 | g3127 | g3127.t1 | cds | g3127.t1.CDS2 | 23079545 | 23080012 |
| chr_3 | g3127 | g3127.t1 | TSS | g3127.t1 | 23080086 | 23080086 |
Sequences
>g3127.t1 Gene=g3127 Length=2055
ATGTTGATTAATTTAGTGAAAAGCAATTTAACATTCCAAAGCCTTAAATCTCACAGTATT
TTCAGAAATCCATTGTCAATTCGTGTAAATATTAAAGATTTAATAAAATCAACAAGACAA
GTGAGAAATACTAGTGGAAATCTATTTTTTAAAAATAGAATATCCTTGACATTTGCAAGT
TTAGGTTTTGGTGCTTCCGTTTATTTTAAAAACGGTCTACCTATCGCAAAATGTGATTAT
TCATCAAGATATAATGGGAGTTTACAGAAAAGTAATGAACTCAAATTCAACTGGAAGTTA
TTTTGGAAATACTTATCAAATCACATCGTTAAATTATTAGGCGCTTGTGTGTTTGCTTTA
GCGGTAGCATATTTAAATATCAATATTCCTTCGTTATTAGGTGTGTTAATTAATACACTT
TCAAAATATGCTGGAGAGCAAATAAATGATGCCAAAAATTTCTTTCAGGATGTTAAAGAG
CCAGCAGTTCGTCTTATTGGTTCTTATATAGCTCAATCGGTATTTACTTTTGGATATATT
TATCTATTGAGTAACATTGGTGAAAACATTGCTATGGAAATACGAAGAGATTTATTTCAA
AATATATTATTGCAAGATATCGAATTCTTTGATAAGAACAAAAGTGGTGAATTGATAAAT
CGAATCACAAATGATGTTCAAGATTTTAAGTCAAGTTTTAAACAAACAATTTCACAAGGC
TTAAGATCTTTAGCAACTTTAATAGGTGGCAGCATAAGTCTATTTTTCATTTCTAGTCAA
CTAGCGTGTATCGCTTTGGTCTCAATTCCTTCGGCAATTCTAATAGGCTCACTACTCGGA
AAATCATTGCGTGAATTAAGTAAACAGTCACAAGCACAAACTGAAAAAGCAACAGGTGTT
TGTTCAGAAGCAATTGGAAACGTGAGGACAGTAAAATCATGTGCGGCAGAACATATCGAA
ATGATGTTTTTTGAAAAAGAAATTGAACATTCAAAAATTCTCTCTCAAAAGCTAGGAGTT
GGAATTGCTGTCTTTCAAGCAATGACAAATTTATTTTTAAATTGTATGGTACTGGGAACA
CTCTATTTTGGAGGAGATTTAATGAGTAATAATTCTATAACTGCCGGAGAGTTAATGGCT
TTTCTTGTTGCATCACAAAGTGTTCAAAGAGCATTATCTCAGGGTTCAATTTTAATGGGA
GTTTTAATTAGAGGAATAACTTCAGGCGCTCGAGTGTTTGAATATATGATGATTGAACCA
AAAGTTGACCTTGTCGAAGGAGCTATAATTAATGATGATAAATTAAAAGGAGAAATTCGT
TTTGAAAACGTCACTTTTTCATACCCGACACGTGCTGATCAAATAGTACTCAAAGATTTC
AATTTGACTTTAAAAGCTGGAAGTACTACAGCACTTGTTGGTTGTTCAGGATCAGGGAAA
TCTACTGTTGGACTTTTACTTGAAAGGTTTTATGAACCGAATCAAGGAAAAATTTATTTA
GATGGAGTAGATTTAAAAGAATTATCGCCCATTTGGTTGAGAAATGAGTTGGGGTACATT
GATCAGCAACCTGTGCTTTTTGCATGTTCTATACTTGATAATATTCGATATGGCAGAGAG
AATGCATCAATTGAAGAAATAATTGAAGCATCAAAAAAATCACAATCACATGATTTTATC
GAAAAATTATCTGATGGGTATGCAACACAAGTAGGTGAAAGAGGAAATCAAATGAGTGGA
GGACAAAAACAACGTATAAGTATTGCGAGGGCATTGATAAAAGATCCAATTATATTGATT
TTGGATGAAGCCACATCAGCACTTGATTCTAATAGCGAAAGAGAAGTGCAAAAAGCACTT
GATAGTGCAATTATAAATAGAACAACATTAATCATAGCACATAGACTTTCAACAATTCGT
AATGCAGATCTCATCGTGCTCATCGATAATGGAAAAATTAAAGAAAAAGGTACTCATGAC
GAATTGATGAAAAAGAAAGGATTGTATTATGAATTAGTACGACAGCAAGAAAGAAAAGAA
CGATCACAAGGATAA
>g3127.t1 Gene=g3127 Length=684
MLINLVKSNLTFQSLKSHSIFRNPLSIRVNIKDLIKSTRQVRNTSGNLFFKNRISLTFAS
LGFGASVYFKNGLPIAKCDYSSRYNGSLQKSNELKFNWKLFWKYLSNHIVKLLGACVFAL
AVAYLNINIPSLLGVLINTLSKYAGEQINDAKNFFQDVKEPAVRLIGSYIAQSVFTFGYI
YLLSNIGENIAMEIRRDLFQNILLQDIEFFDKNKSGELINRITNDVQDFKSSFKQTISQG
LRSLATLIGGSISLFFISSQLACIALVSIPSAILIGSLLGKSLRELSKQSQAQTEKATGV
CSEAIGNVRTVKSCAAEHIEMMFFEKEIEHSKILSQKLGVGIAVFQAMTNLFLNCMVLGT
LYFGGDLMSNNSITAGELMAFLVASQSVQRALSQGSILMGVLIRGITSGARVFEYMMIEP
KVDLVEGAIINDDKLKGEIRFENVTFSYPTRADQIVLKDFNLTLKAGSTTALVGCSGSGK
STVGLLLERFYEPNQGKIYLDGVDLKELSPIWLRNELGYIDQQPVLFACSILDNIRYGRE
NASIEEIIEASKKSQSHDFIEKLSDGYATQVGERGNQMSGGQKQRISIARALIKDPIILI
LDEATSALDSNSEREVQKALDSAIINRTTLIIAHRLSTIRNADLIVLIDNGKIKEKGTHD
ELMKKKGLYYELVRQQERKERSQG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 20 | g3127.t1 | CDD | cd18574 | ABC_6TM_ABCB8_like | 115 | 412 | 5.10033E-146 |
| 21 | g3127.t1 | CDD | cd03249 | ABC_MTABC3_MDL1_MDL2 | 439 | 676 | 2.38154E-138 |
| 8 | g3127.t1 | Gene3D | G3DSA:1.20.1560.10 | - | 96 | 430 | 7.3E-77 |
| 7 | g3127.t1 | Gene3D | G3DSA:3.40.50.300 | - | 431 | 683 | 8.2E-94 |
| 3 | g3127.t1 | PANTHER | PTHR24221 | ATP-BINDING CASSETTE SUB-FAMILY B | 115 | 674 | 1.0E-196 |
| 4 | g3127.t1 | PANTHER | PTHR24221:SF167 | ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 8, MITOCHONDRIAL | 115 | 674 | 1.0E-196 |
| 1 | g3127.t1 | Pfam | PF00664 | ABC transporter transmembrane region | 114 | 390 | 3.8E-50 |
| 2 | g3127.t1 | Pfam | PF00005 | ABC transporter | 457 | 606 | 3.8E-31 |
| 11 | g3127.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 108 | - |
| 15 | g3127.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 109 | 127 | - |
| 12 | g3127.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 128 | 161 | - |
| 16 | g3127.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 162 | 182 | - |
| 9 | g3127.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 183 | 239 | - |
| 19 | g3127.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 258 | - |
| 14 | g3127.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 259 | 263 | - |
| 18 | g3127.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 264 | 280 | - |
| 10 | g3127.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 281 | 337 | - |
| 17 | g3127.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 338 | 363 | - |
| 13 | g3127.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 364 | 684 | - |
| 25 | g3127.t1 | ProSitePatterns | PS00211 | ABC transporters family signature. | 578 | 592 | - |
| 27 | g3127.t1 | ProSiteProfiles | PS50929 | ABC transporter integral membrane type-1 fused domain profile. | 113 | 404 | 47.731 |
| 28 | g3127.t1 | ProSiteProfiles | PS50893 | ATP-binding cassette, ABC transporter-type domain profile. | 439 | 675 | 25.037 |
| 26 | g3127.t1 | SMART | SM00382 | AAA_5 | 466 | 652 | 1.3E-14 |
| 5 | g3127.t1 | SUPERFAMILY | SSF90123 | ABC transporter transmembrane region | 99 | 420 | 4.71E-64 |
| 6 | g3127.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 434 | 676 | 9.78E-88 |
| 22 | g3127.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 112 | 134 | - |
| 23 | g3127.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 187 | - |
| 24 | g3127.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 252 | 274 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0055085 | transmembrane transport | BP |
| GO:0005524 | ATP binding | MF |
| GO:0042626 | ATPase-coupled transmembrane transporter activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05655 (ATP-binding cassette, subfamily B (MDR/TAP), member 8)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
