General information for g3203.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Cathepsin B-like cysteine proteinase 2.
Parent gene
- The parent gene g3203 is located on chr_3: 23644273..23648900 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3203 | g3203.t1 | isoform | g3203.t1 | 23644273 | 23648900 |
| chr_3 | g3203 | g3203.t1 | exon | g3203.t1.exon1 | 23644273 | 23644283 |
| chr_3 | g3203 | g3203.t1 | cds | g3203.t1.CDS1 | 23644273 | 23644283 |
| chr_3 | g3203 | g3203.t1 | exon | g3203.t1.exon2 | 23644344 | 23644833 |
| chr_3 | g3203 | g3203.t1 | cds | g3203.t1.CDS2 | 23644344 | 23644833 |
| chr_3 | g3203 | g3203.t1 | exon | g3203.t1.exon3 | 23644934 | 23644965 |
| chr_3 | g3203 | g3203.t1 | cds | g3203.t1.CDS3 | 23644934 | 23644965 |
| chr_3 | g3203 | g3203.t1 | exon | g3203.t1.exon4 | 23648535 | 23648746 |
| chr_3 | g3203 | g3203.t1 | cds | g3203.t1.CDS4 | 23648535 | 23648746 |
| chr_3 | g3203 | g3203.t1 | exon | g3203.t1.exon5 | 23648806 | 23648900 |
| chr_3 | g3203 | g3203.t1 | cds | g3203.t1.CDS5 | 23648806 | 23648900 |
| chr_3 | g3203 | g3203.t1 | TSS | g3203.t1 | NA | NA |
| chr_3 | g3203 | g3203.t1 | TTS | g3203.t1 | NA | NA |
Sequences
>g3203.t1 Gene=g3203 Length=840
ATGAAACTCAGAATTGCTTTCATATTTTGTTTAGCAATAATTATTCTCCAATCATTTGCA
AATGCTAAAAAATCATCGTCATCTGTAAATTTCTATCAAATAATTTTTAAAATTTTTAAA
AATACCAATTCATACACTCAGTCAGATTTAAATGAAAAACTCACAAATTTTCACACTCAA
AGTAAGTCAAATTTTGATAATGTTTTGACTGATTATAAAGTCAAGCTCGACAACTACGAG
AAAAGAAAGCAAATTTTTGATGAAAATTATGAAGAAATTCGTGAACACAATAAACGATAT
GATTTGGGACTTGAAAGTTTTGAGCTTGGTGTTAATCAATTTTCACATTTAAGTGAAACT
GAAAGAATAAAATATTTGAGCAAAATTAATCCAAGTGAAAATGAAAATTTAACAACTGAA
GTCAATAAATTAAAGCCAAAATTTTCTGATGTTCCTGAAAGTTACGATTGGAGAAACAAA
AATGTTTTTCATCCTGTTCAGTTATTCAATTTTTCAGGACCAAGGCCAATGTGTGGAAAT
TCAGAATTTGACATTAGATCAGCAATTTATGAAAAAGGACCTGTCACTGCTAGTTTCGAA
GTTTATAAAAGTTTTTATCAATATAACAATGGAATATATGAGAGAAAAGAAAATGAAAAT
GGTGTTCAAGGTTATCATGCAGTGGTAATTGTTGGATTTGGAGAAGATAATGGCAAAAAA
TATTGGATTGTTAGAAACTCTTGGGGTTCAAAATGGGGAGAGCAAGGTTATTTTAGAATC
ATCAGAGGTTCAGACAACTGCAAATTTGAATCAACTGCTCATTATTTACGACAGATCTAA
>g3203.t1 Gene=g3203 Length=279
MKLRIAFIFCLAIIILQSFANAKKSSSSVNFYQIIFKIFKNTNSYTQSDLNEKLTNFHTQ
SKSNFDNVLTDYKVKLDNYEKRKQIFDENYEEIREHNKRYDLGLESFELGVNQFSHLSET
ERIKYLSKINPSENENLTTEVNKLKPKFSDVPESYDWRNKNVFHPVQLFNFSGPRPMCGN
SEFDIRSAIYEKGPVTASFEVYKSFYQYNNGIYERKENENGVQGYHAVVIVGFGEDNGKK
YWIVRNSWGSKWGEQGYFRIIRGSDNCKFESTAHYLRQI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g3203.t1 | Coils | Coil | Coil | 69 | 96 | - |
| 9 | g3203.t1 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 44 | 170 | 1.4E-16 |
| 8 | g3203.t1 | Gene3D | G3DSA:3.90.70.10 | Cysteine proteinases | 171 | 278 | 5.1E-38 |
| 3 | g3203.t1 | PANTHER | PTHR12411:SF737 | CATHEPSIN B | 181 | 274 | 6.1E-25 |
| 4 | g3203.t1 | PANTHER | PTHR12411 | CYSTEINE PROTEASE FAMILY C1-RELATED | 181 | 274 | 6.1E-25 |
| 1 | g3203.t1 | Pfam | PF08246 | Cathepsin propeptide inhibitor domain (I29) | 71 | 121 | 2.5E-7 |
| 2 | g3203.t1 | Pfam | PF00112 | Papain family cysteine protease | 180 | 275 | 4.5E-30 |
| 12 | g3203.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 13 | g3203.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 14 | g3203.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 15 | g3203.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 22 | - |
| 11 | g3203.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 279 | - |
| 18 | g3203.t1 | ProSitePatterns | PS00639 | Eukaryotic thiol (cysteine) proteases histidine active site. | 224 | 234 | - |
| 17 | g3203.t1 | ProSitePatterns | PS00640 | Eukaryotic thiol (cysteine) proteases asparagine active site. | 241 | 260 | - |
| 20 | g3203.t1 | SMART | SM00848 | Inhibitor_I29_2 | 65 | 122 | 7.7E-7 |
| 19 | g3203.t1 | SMART | SM00645 | pept_c1 | 151 | 277 | 4.8E-8 |
| 5 | g3203.t1 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 75 | 275 | 9.83E-49 |
| 7 | g3203.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 16 | g3203.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 6 | g3203.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008234 | cysteine-type peptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed