General information for g3275.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Lipase member H-A.
Parent gene
- The parent gene g3275 is located on chr_3: 24133909..24135410 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3275 | g3275.t2 | isoform | g3275.t2 | 24133909 | 24135410 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon1 | 24133909 | 24133951 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS1 | 24133909 | 24133951 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon2 | 24134013 | 24134035 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS2 | 24134013 | 24134035 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon3 | 24134150 | 24134434 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS3 | 24134150 | 24134434 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon4 | 24134536 | 24134710 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS4 | 24134536 | 24134710 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon5 | 24134768 | 24134846 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS5 | 24134768 | 24134846 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon6 | 24134965 | 24135107 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS6 | 24134965 | 24135107 |
| chr_3 | g3275 | g3275.t2 | exon | g3275.t2.exon7 | 24135175 | 24135410 |
| chr_3 | g3275 | g3275.t2 | cds | g3275.t2.CDS7 | 24135175 | 24135410 |
| chr_3 | g3275 | g3275.t2 | TSS | g3275.t2 | NA | NA |
| chr_3 | g3275 | g3275.t2 | TTS | g3275.t2 | NA | NA |
Sequences
>g3275.t2 Gene=g3275 Length=984
ATGAAATTTTTTATTTTTATAATTTTTCATTCTGTGCTGTGTTTGCTCCAAGTTAATTCT
TTAGTTGTCGATGGAGGAATTAATTTTCTCTTCTTTGGCAGTAATTTTTCTGACTATGTC
AGTACAACATTAAATTATAATTTCACAAGTCTCCAAAATACAAAATATTTCAATATCAAT
AAGCCAACTGCAGTTTTTCTTCATGGTTGGGGAACTTCCTTCGTAGCATCTGTTAATCAA
GCTTTAGCTGTTGCTTTCATCAGTAGAGGCGATTACAATACAATTATTGTTGATTGGAGT
AAATATGCATCAGATCCCGATTATTATGAAGTTGCAGCTTCGATTAATGATCAAGCAACT
TTCATTGTTCAAGTTCTAATTCAAATGCAAAATGCTGGTTACAATTTAACATCTATTTAC
TTCATGGGTCATAGTTTAGGAGCAAATATTCTTGGTCGCGTTGGTTATCAACTCATTAAA
AATTATAATTTTACTCCTACAAGAATTTCTGGCATTGAACCAACTGGTGTATTTTTTGGA
AATTTCAATGAATGGCCTACAGGAATTTCTTTACTTTTTCCTATACTCAACTTTCTCAAT
GCAAAATTTGTCGACATAATTCATACTGACATATATGGTCAAGGTGAAAATTATCCAGTT
GGTCATATGGATTTTTGGCCTAATTTTGGTCATGATCAGCCTGCTTGCAGTATTAATCCA
TTTTATTTCACATGCAATCCTTGGAATGATAACTGTCTTAATTGCAATCACATTCGTTCC
TTGAAATATTTCACTGAATCTGTTAGTTCAGGTTTAGTAAGAAAATTTAAATCACGTTTG
TGTAATGGTACTAATTATTTCACTAGTGCTTGCTATGCTGTTGTAGCATCAATGGGTTTT
TATGCTGACAATTATGCTAATAATCCTGGAAATTATTTCCTTACAACTACTGGTGTTTCA
CCTTATTCTGTTTCGAATTTTTAA
>g3275.t2 Gene=g3275 Length=327
MKFFIFIIFHSVLCLLQVNSLVVDGGINFLFFGSNFSDYVSTTLNYNFTSLQNTKYFNIN
KPTAVFLHGWGTSFVASVNQALAVAFISRGDYNTIIVDWSKYASDPDYYEVAASINDQAT
FIVQVLIQMQNAGYNLTSIYFMGHSLGANILGRVGYQLIKNYNFTPTRISGIEPTGVFFG
NFNEWPTGISLLFPILNFLNAKFVDIIHTDIYGQGENYPVGHMDFWPNFGHDQPACSINP
FYFTCNPWNDNCLNCNHIRSLKYFTESVSSGLVRKFKSRLCNGTNYFTSACYAVVASMGF
YADNYANNPGNYFLTTTGVSPYSVSNF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g3275.t2 | Gene3D | G3DSA:3.40.50.1820 | - | 15 | 323 | 9.7E-60 |
| 2 | g3275.t2 | PANTHER | PTHR11610 | LIPASE | 43 | 323 | 9.4E-55 |
| 5 | g3275.t2 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 51 | 70 | 1.8E-10 |
| 4 | g3275.t2 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 138 | 156 | 1.8E-10 |
| 3 | g3275.t2 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 255 | 270 | 1.8E-10 |
| 1 | g3275.t2 | Pfam | PF00151 | Lipase | 49 | 322 | 4.5E-35 |
| 10 | g3275.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 11 | g3275.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g3275.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 16 | - |
| 13 | g3275.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 9 | g3275.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 327 | - |
| 6 | g3275.t2 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 20 | 322 | 3.67E-43 |
| 7 | g3275.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004806 | triglyceride lipase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed