General information for g3305.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g3305 is located on chr_3: 24456973..24460371 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3305 | g3305.t1 | isoform | g3305.t1 | 24456973 | 24460371 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon1 | 24456973 | 24457813 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS1 | 24456973 | 24457813 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon2 | 24457872 | 24458351 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS2 | 24457872 | 24458351 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon3 | 24458404 | 24458754 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS3 | 24458404 | 24458754 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon4 | 24458812 | 24459096 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS4 | 24458812 | 24459096 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon5 | 24459152 | 24459718 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS5 | 24459152 | 24459718 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon6 | 24459868 | 24460239 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS6 | 24459868 | 24460239 |
| chr_3 | g3305 | g3305.t1 | exon | g3305.t1.exon7 | 24460298 | 24460371 |
| chr_3 | g3305 | g3305.t1 | cds | g3305.t1.CDS7 | 24460298 | 24460371 |
| chr_3 | g3305 | g3305.t1 | TSS | g3305.t1 | NA | NA |
| chr_3 | g3305 | g3305.t1 | TTS | g3305.t1 | NA | NA |
Sequences
>g3305.t1 Gene=g3305 Length=2970
ATGGAAAAATTCTTCGAAAATTCAACAATTCATGGATTTAAATATTTGTCAAAAAAATTT
CATTGGGTTGAAAGGATTTTTTGGTTTATTTCTTTGTTTCTGTCATTAGTTCTAACAATA
GTTCTCATCACGAAACTCGTCGAAAAAATCAAAAGCATACCAGTAATTGTGGAAATTTCA
GACGAGCAAGTCCACGTCAGTGAAATTGACTTTCCAGCTATTACACAATGTTATGGACTA
ATTCCTCGTTTTCCATACATTTTTCAAAATAATCAATATAAATATTTTCGTTACGAACGA
AATACTAACTTACATTATTATTATGAAAAAGAAGAAATTGATGAAGAATCAAGAGATGAA
AAGAAAATGGGAAATGTTGGATATATTAATAATCTTATGAAACTAGAATCTGGAGTGATA
AAAGCTAAAAATTTAGGATTAAAAGCTTTAAAGCGACTTCAAGTGATTGATTTGATTTCT
CGTCGTGGAAGTTTTGCAAAAATCAATTTTTCAGTTCCAACTGATGATTTTCTTGATGTT
ATAAATGAATTTACAATGCAAGCTTCGTTAGAATCATCACTTATTGCTTTCTCTCAAACA
TACAGTGGTCCGTCGATAGTTTCAAATATCATTACTCAAACAAACTTGTGCAAAAATTAT
AACATTGCTTTAGCTGATGACGTTTTTCATACAAACATAACTTCTAATGACTTTAATTTT
CAACATTATAATGACAGAAGAGGAAAATTTTATAAAAACTTTACAATTGTTGTTCCACAA
AGATCATTGACATCAAAAATTTTTCTTCTTGTCCGAATATTAACGTACAACTTACATCGG
GAACATTCATTAGGTTCAACTAAAGGAGGTTTAATGTATTTTCACGATCCTTATGAAATG
CCATCAAAGTTTACACAATCAATGATGATTAATGAAAACCAATATAATGAAATTTTTGTT
GAACCACAAATGATGGAAGTTGATGAATCATTTTATGATGAAAATCCTCTTGAACGTAAC
TGTTATTTTGATGATGAACGAAAGTTAAATTTCTTTAAAGTTTACACAAAACCAAATTGT
TTGTTCGAGTGCATGTCAGAATTCATGGCACAAAAATGTGGTTGTGTTGAATTTTTCATG
ATTCGAAATAAAACAACAAGAATTTGCTCAGCAAGCGAAAAAATTTGTTATGATAAAGTT
AGAGATGAATTTGACACTCAAATAAATGATTGTGGATGTTTGGAAAGATGCAATTATGTC
AAATATAATTTTGAATATAATGTTCGAGAAAGAAATACTAAAGATTTACAAAAAGTTAAT
AGTGCTGGTTTAAATGGAATGATGCAAACAAACAGCAACATTATGTATAAAAGTGTCTTT
TTTAATAAATTCATACGCAAGAGACAATTCAATGAAATTGATTTTCTTTCATTTGTTGGT
GGACTTTTAGGACTTTTTGCTGGATTTTCTGCTTTGTCTTTTATTGAAATATTTTATTGG
ATTCTTAGAAAAATCTTAATTAAAAGAACAGCTATTGTTCATCCATTGTATACAAAACAA
GAAGGATCTGTAACAATGTTCAATAATTTTCTTGATAATTCATCAATCCATGGTGTTAAT
TATCTCAATAAGAATAAACTTTTTCATAAACTTTTTTGGACATTTTTCATCACACTTTCA
TTAACTTTAAGTGCTTTAATGATCAAAAATATCAAAAAACAAACTTGGGCAAAATCGTAC
GATGACAGTTACAATTTTAAAGATAGAGTTTTATTTCCAGCTGTAACATTTATTCCAAAT
TATTATGCTGATGTGAATTTGCAAGAATTGATTGATAGCCAAAGAATAAAAATTAAAAAT
ATCACATTATTACAAGAATTATACAATAAAAACATCACAGAACAGTTTGAATGCATTTAT
TTGATACAATATAAGTTTTTCAATGATAATAAAACCAAACCTTATAATTTGGAATTTATG
CGACGACATTCAAAAGTTGATTATTTTCAACAGCAATATGCAACATGGAATGATAAATAT
CAAGTAAATTTTACGGAAGTTAGAACTTTTTATGGACTTGCTTATAGTTTCAATCTTTTA
GATTCATCGAAACTTCTTCATTATAATGAAGTTCATGATGAATTTAAATACAAACAAAAC
ATATCAATGATTAATCAAGCTTCAATGCCAGAAATTTCAATTGAAAATTATCCACTTAAA
GTCAATCTCAAAAGGCAATCAAAACTTACTTTAAACGTTAATGAAGAAAAATTTGAAAAT
CCAAATATGAAAGTTTGCATAGGAATGTCTTTAAGAGTTCATTTACCAACTGATCTACCA
CAATTTGTATATTTTGAAGGCTTTGGAGAAATTAATCATTCTACGGATGTTGAAATTATC
CCAGAAATTACAAGAACTGATGATAGTTTAAAAGTTTTAACACCCAAAGAGCGAGAATGT
TATTTTGAAAATGAAAGAAAATTGAAATATTTCAAAATTTATACTGAGGAATATTGCAAG
ATGGAATGTATAATTGAATCCACAATTCAGTATTGTAAATGTGTTGATTCATTGTTTGTT
TATGGACCTAAACATGAAAATCTAGGATTTTGTCAGTGGCAAAAAATGGGTTGTGTTGGG
ACAACTCGTAATTATTATTCTATGTACTTTAATAAAATGATTGATGAGGACTGCAATTGC
TTGCCAACTTGTAATTCATTGACTTATCATATGAAATTTTATCCTCGAGATGATGAAAAC
AATTCAATTGTGATAAATGTCAAAATGAACACAGATCAATTGATTTTGTATACAAGGTTT
CATTTCTTTACATTTTCTGATGCTGTTTCTTATGTTGGTGGTTTGCTTGGACTTTTTGCT
GGAATTTCAGTTTTATCAATTGTTGAAATCTTTTATTTTATTACATTGCGATTGTTGAAT
GATATTTTGAGATTGTTAAAACGAACATAA
>g3305.t1 Gene=g3305 Length=989
MEKFFENSTIHGFKYLSKKFHWVERIFWFISLFLSLVLTIVLITKLVEKIKSIPVIVEIS
DEQVHVSEIDFPAITQCYGLIPRFPYIFQNNQYKYFRYERNTNLHYYYEKEEIDEESRDE
KKMGNVGYINNLMKLESGVIKAKNLGLKALKRLQVIDLISRRGSFAKINFSVPTDDFLDV
INEFTMQASLESSLIAFSQTYSGPSIVSNIITQTNLCKNYNIALADDVFHTNITSNDFNF
QHYNDRRGKFYKNFTIVVPQRSLTSKIFLLVRILTYNLHREHSLGSTKGGLMYFHDPYEM
PSKFTQSMMINENQYNEIFVEPQMMEVDESFYDENPLERNCYFDDERKLNFFKVYTKPNC
LFECMSEFMAQKCGCVEFFMIRNKTTRICSASEKICYDKVRDEFDTQINDCGCLERCNYV
KYNFEYNVRERNTKDLQKVNSAGLNGMMQTNSNIMYKSVFFNKFIRKRQFNEIDFLSFVG
GLLGLFAGFSALSFIEIFYWILRKILIKRTAIVHPLYTKQEGSVTMFNNFLDNSSIHGVN
YLNKNKLFHKLFWTFFITLSLTLSALMIKNIKKQTWAKSYDDSYNFKDRVLFPAVTFIPN
YYADVNLQELIDSQRIKIKNITLLQELYNKNITEQFECIYLIQYKFFNDNKTKPYNLEFM
RRHSKVDYFQQQYATWNDKYQVNFTEVRTFYGLAYSFNLLDSSKLLHYNEVHDEFKYKQN
ISMINQASMPEISIENYPLKVNLKRQSKLTLNVNEEKFENPNMKVCIGMSLRVHLPTDLP
QFVYFEGFGEINHSTDVEIIPEITRTDDSLKVLTPKERECYFENERKLKYFKIYTEEYCK
MECIIESTIQYCKCVDSLFVYGPKHENLGFCQWQKMGCVGTTRNYYSMYFNKMIDEDCNC
LPTCNSLTYHMKFYPRDDENNSIVINVKMNTDQLILYTRFHFFTFSDAVSYVGGLLGLFA
GISVLSIVEIFYFITLRLLNDILRLLKRT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g3305.t1 | Gene3D | G3DSA:1.10.287.820 | - | 336 | 417 | 9.5E-7 |
| 16 | g3305.t1 | Gene3D | G3DSA:1.10.287.770 | - | 551 | 971 | 3.7E-21 |
| 12 | g3305.t1 | Gene3D | G3DSA:2.60.470.10 | - | 580 | 941 | 3.7E-21 |
| 13 | g3305.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 600 | 768 | 3.7E-21 |
| 15 | g3305.t1 | Gene3D | G3DSA:1.10.287.820 | - | 812 | 904 | 3.7E-21 |
| 3 | g3305.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 3 | 503 | 2.5E-80 |
| 5 | g3305.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 3 | 503 | 2.5E-80 |
| 4 | g3305.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 526 | 981 | 2.5E-80 |
| 6 | g3305.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 526 | 981 | 2.5E-80 |
| 2 | g3305.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 4 | 499 | 8.3E-59 |
| 1 | g3305.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 530 | 973 | 3.6E-49 |
| 22 | g3305.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 25 | - |
| 26 | g3305.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 26 | 44 | - |
| 17 | g3305.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 45 | 474 | - |
| 25 | g3305.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 475 | 501 | - |
| 21 | g3305.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 502 | 550 | - |
| 24 | g3305.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 551 | 568 | - |
| 19 | g3305.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 569 | 933 | - |
| 27 | g3305.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 934 | 952 | - |
| 20 | g3305.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 953 | 957 | - |
| 23 | g3305.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 958 | 979 | - |
| 18 | g3305.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 980 | 989 | - |
| 11 | g3305.t1 | ProSitePatterns | PS01206 | Amiloride-sensitive sodium channels signature. | 355 | 375 | - |
| 9 | g3305.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 21 | 43 | - |
| 7 | g3305.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 480 | 502 | - |
| 8 | g3305.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 551 | 568 | - |
| 10 | g3305.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 952 | 974 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed