General information for g3343.t32
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 60S ribosomal protein L4.
Parent gene
- The parent gene g3343 is located on chr_3: 24754125..24754849 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3343 | g3343.t32 | TTS | g3343.t32 | 24752884 | 24752884 |
| chr_3 | g3343 | g3343.t32 | isoform | g3343.t32 | 24753005 | 24754773 |
| chr_3 | g3343 | g3343.t32 | exon | g3343.t32.exon1 | 24753005 | 24753703 |
| chr_3 | g3343 | g3343.t32 | cds | g3343.t32.CDS1 | 24753005 | 24753703 |
| chr_3 | g3343 | g3343.t32 | exon | g3343.t32.exon2 | 24753938 | 24754053 |
| chr_3 | g3343 | g3343.t32 | cds | g3343.t32.CDS2 | 24753938 | 24754053 |
| chr_3 | g3343 | g3343.t32 | exon | g3343.t32.exon3 | 24754107 | 24754533 |
| chr_3 | g3343 | g3343.t32 | cds | g3343.t32.CDS3 | 24754107 | 24754533 |
| chr_3 | g3343 | g3343.t32 | exon | g3343.t32.exon4 | 24754771 | 24754773 |
| chr_3 | g3343 | g3343.t32 | cds | g3343.t32.CDS4 | 24754771 | 24754773 |
| chr_3 | g3343 | g3343.t32 | TSS | g3343.t32 | 24754851 | 24754851 |
Sequences
>g3343.t32 Gene=g3343 Length=1245
ATGAGTAAATCAGCTGCTCGTCCGCTCGTCAGTGTTTATACTGACAAAAGTGAGGTTATT
AAAGATACAACAATTCCATTGCCTGCAATTTTCAAGGCTCCAATTCGCCCAGATGTCGTC
AATGATGTACATCAATTAATGCGTCGTAATGCTCGTCAAGCATACGCTGTTAGCGCTGAA
GCAGGCCATCAAACATCAGCTGAATCATGGGGTACAGGTCGTGCTGTTGCTCGTATTCCT
CGAGTTCGTGGTGGTGGTACTCATCGTTCAGGTCAGGGTGCTTTCGGAAATATGTGTCGT
GGAGGTCGCATGTTCGCTCCAACTAAACCATGGCGTAGATGGCATCGTCGCATTAATGTC
AACTTGAAACGTTATGCAGTTGTTTCAGCTGTTGCTGCATCAGGCGTTCCAGCTCTTGTT
CAAGCTCGAGGACATATTATTGACGGAATTGCTGAACTTCCATTGGTTGTCTCAGATAAA
GTTCAAGAATACAACAAGACCAAACAAGCTGTTTTGTTCTTACGTCGTAACAAGTTGTGG
GGAGATGTTTATCGTTCAAAACGCTTTAGAGCTGGTCGTGGTAAAATGCGAAATCGTCGT
CGTATTCAACGTCGTGGACCATTAATTGTTTACTCGAAGGATGATGGCTTGCGTCGTGCA
TTCCGTAATATTCCCGGTGTTGATACAATGAGCGTTCACAAATTGAATTTGCTCAAATTG
GCTCCAGGTGGACATCTTGGACGATTGATTGTTTGGACCGAAGGCGCTTTCAAGCATTTG
AATGCATTGTTTGGAACATGGAGTGAGCCTTCAACAGTGAAAAAGGCTTACAATCTGCCA
CATCCAATTATGTCTAACACTGATTTGACTCGCTTGCTCAAATCACAAGAAATCAAGAGC
GTTTTGAATGCACCTAAGAAGAGAGTTTTCCATCGTGTACGCCGCTTGAATCCATTGAAG
AACGCCAGACAAAAGACCAAGTTGAATCCATATTCAGAAGTTACTCGTCGTCGTGCATTG
TTGGCTAAAGAAAAGCGAAAGTATGAGACACAAATTGCCAAGGCAAAGAAGAAGAAGGTC
GAACTCAAGAGCAATCATCCAGCTATTGTGTATCAAAAGAATGTTGCTAATCGTTTGAAG
CTCTTAGCTGCATCAAAGAAAGCTAGACAAGCTCGCGCTGCTGAAAAGAAAAAAGCTGCT
GCTGGAAAGAAAGCAACAAAAAAGACCGCAGCCAAGAAAAAGTAA
>g3343.t32 Gene=g3343 Length=414
MSKSAARPLVSVYTDKSEVIKDTTIPLPAIFKAPIRPDVVNDVHQLMRRNARQAYAVSAE
AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHRRINV
NLKRYAVVSAVAASGVPALVQARGHIIDGIAELPLVVSDKVQEYNKTKQAVLFLRRNKLW
GDVYRSKRFRAGRGKMRNRRRIQRRGPLIVYSKDDGLRRAFRNIPGVDTMSVHKLNLLKL
APGGHLGRLIVWTEGAFKHLNALFGTWSEPSTVKKAYNLPHPIMSNTDLTRLLKSQEIKS
VLNAPKKRVFHRVRRLNPLKNARQKTKLNPYSEVTRRRALLAKEKRKYETQIAKAKKKKV
ELKSNHPAIVYQKNVANRLKLLAASKKARQARAAEKKKAAAGKKATKKTAAKKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g3343.t32 | Coils | Coil | Coil | 338 | 365 | - |
| 8 | g3343.t32 | Gene3D | G3DSA:3.40.1370.10 | - | 4 | 304 | 3.0E-131 |
| 7 | g3343.t32 | MobiDBLite | mobidb-lite | consensus disorder prediction | 386 | 414 | - |
| 3 | g3343.t32 | PANTHER | PTHR19431 | 60S RIBOSOMAL PROTEIN L4 | 4 | 394 | 4.2E-166 |
| 4 | g3343.t32 | PANTHER | PTHR19431:SF0 | 60S RIBOSOMAL PROTEIN L4 | 4 | 394 | 4.2E-166 |
| 1 | g3343.t32 | Pfam | PF00573 | Ribosomal protein L4/L1 family | 25 | 261 | 1.8E-39 |
| 2 | g3343.t32 | Pfam | PF14374 | 60S ribosomal protein L4 C-terminal domain | 275 | 347 | 1.3E-23 |
| 6 | g3343.t32 | ProSitePatterns | PS00939 | Ribosomal protein L1e signature. | 119 | 145 | - |
| 5 | g3343.t32 | SUPERFAMILY | SSF52166 | Ribosomal protein L4 | 9 | 261 | 3.4E-79 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005840 | ribosome | CC |
| GO:0006412 | translation | BP |
| GO:0003735 | structural constituent of ribosome | MF |
KEGG
Orthology
- This transcript has been annotated with K02930 (large subunit ribosomal protein L4e)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed