General information for g3343.t59
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 60S ribosomal protein L4.
Parent gene
- The parent gene g3343 is located on chr_3: 24754125..24754849 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3343 | g3343.t59 | TTS | g3343.t59 | 24752884 | 24752884 |
| chr_3 | g3343 | g3343.t59 | isoform | g3343.t59 | 24753005 | 24754849 |
| chr_3 | g3343 | g3343.t59 | exon | g3343.t59.exon1 | 24753005 | 24753703 |
| chr_3 | g3343 | g3343.t59 | cds | g3343.t59.CDS1 | 24753005 | 24753703 |
| chr_3 | g3343 | g3343.t59 | exon | g3343.t59.exon2 | 24753929 | 24754053 |
| chr_3 | g3343 | g3343.t59 | cds | g3343.t59.CDS2 | 24753929 | 24754053 |
| chr_3 | g3343 | g3343.t59 | exon | g3343.t59.exon3 | 24754107 | 24754533 |
| chr_3 | g3343 | g3343.t59 | cds | g3343.t59.CDS3 | 24754107 | 24754398 |
| chr_3 | g3343 | g3343.t59 | exon | g3343.t59.exon4 | 24754779 | 24754849 |
| chr_3 | g3343 | g3343.t59 | TSS | g3343.t59 | 24754851 | 24754851 |
Sequences
>g3343.t59 Gene=g3343 Length=1322
TTTTCCTTGCGTTCCCGTGCGCATTAATTCTGTCAAAAGAAAATAATCATCCAAAAACGG
GTTGATAACTCAGTAAATCAGCTGCTCGTCCGCTCGTCAGTGTTTATACTGACAAAAGTG
AGGTTATTAAAGATACAACAATTCCATTGCCTGCAATTTTCAAGGCTCCAATTCGCCCAG
ATGTCGTCAATGATGTACATCAATTAATGCGTCGTAATGCTCGTCAAGCATACGCTGTTA
GCGCTGAAGCAGGCCATCAAACATCAGCTGAATCATGGGGTACAGGTCGTGCTGTTGCTC
GTATTCCTCGAGTTCGTGGTGGTGGTACTCATCGTTCAGGTCAGGGTGCTTTCGGAAATA
TGTGTCGTGGAGGTCGCATGTTCGCTCCAACTAAACCATGGCGTAGATGGCATCGTCGCA
TTAATGTCAACTTGAAACGTTATGCAGTTGTTTCAGCTGTTGCTGCATCAGGCGTTCCAG
CTCTTGTTCAAGCTCGAGGACATATTATTGACGGAATTGCTGAACTTCCATTGGTTGTCT
CAGATAAAGTTCAAGAATACAACAAGACCAAACAAGCTGTTTTGTTCTTACGTCGTAACA
AGTTGTGGGGAGATGTTTTGAAAGTTTATCGTTCAAAACGCTTTAGAGCTGGTCGTGGTA
AAATGCGAAATCGTCGTCGTATTCAACGTCGTGGACCATTAATTGTTTACTCGAAGGATG
ATGGCTTGCGTCGTGCATTCCGTAATATTCCCGGTGTTGATACAATGAGCGTTCACAAAT
TGAATTTGCTCAAATTGGCTCCAGGTGGACATCTTGGACGATTGATTGTTTGGACCGAAG
GCGCTTTCAAGCATTTGAATGCATTGTTTGGAACATGGAGTGAGCCTTCAACAGTGAAAA
AGGCTTACAATCTGCCACATCCAATTATGTCTAACACTGATTTGACTCGCTTGCTCAAAT
CACAAGAAATCAAGAGCGTTTTGAATGCACCTAAGAAGAGAGTTTTCCATCGTGTACGCC
GCTTGAATCCATTGAAGAACGCCAGACAAAAGACCAAGTTGAATCCATATTCAGAAGTTA
CTCGTCGTCGTGCATTGTTGGCTAAAGAAAAGCGAAAGTATGAGACACAAATTGCCAAGG
CAAAGAAGAAGAAGGTCGAACTCAAGAGCAATCATCCAGCTATTGTGTATCAAAAGAATG
TTGCTAATCGTTTGAAGCTCTTAGCTGCATCAAAGAAAGCTAGACAAGCTCGCGCTGCTG
AAAAGAAAAAAGCTGCTGCTGGAAAGAAAGCAACAAAAAAGACCGCAGCCAAGAAAAAGT
AA
>g3343.t59 Gene=g3343 Length=371
MRRNARQAYAVSAEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFA
PTKPWRRWHRRINVNLKRYAVVSAVAASGVPALVQARGHIIDGIAELPLVVSDKVQEYNK
TKQAVLFLRRNKLWGDVLKVYRSKRFRAGRGKMRNRRRIQRRGPLIVYSKDDGLRRAFRN
IPGVDTMSVHKLNLLKLAPGGHLGRLIVWTEGAFKHLNALFGTWSEPSTVKKAYNLPHPI
MSNTDLTRLLKSQEIKSVLNAPKKRVFHRVRRLNPLKNARQKTKLNPYSEVTRRRALLAK
EKRKYETQIAKAKKKKVELKSNHPAIVYQKNVANRLKLLAASKKARQARAAEKKKAAAGK
KATKKTAAKKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g3343.t59 | Coils | Coil | Coil | 295 | 322 | - |
| 8 | g3343.t59 | Gene3D | G3DSA:3.40.1370.10 | - | 1 | 261 | 2.5E-119 |
| 7 | g3343.t59 | MobiDBLite | mobidb-lite | consensus disorder prediction | 343 | 371 | - |
| 3 | g3343.t59 | PANTHER | PTHR19431 | 60S RIBOSOMAL PROTEIN L4 | 1 | 351 | 7.7E-151 |
| 4 | g3343.t59 | PANTHER | PTHR19431:SF0 | 60S RIBOSOMAL PROTEIN L4 | 1 | 351 | 7.7E-151 |
| 1 | g3343.t59 | Pfam | PF00573 | Ribosomal protein L4/L1 family | 2 | 218 | 8.3E-32 |
| 2 | g3343.t59 | Pfam | PF14374 | 60S ribosomal protein L4 C-terminal domain | 232 | 304 | 1.1E-23 |
| 6 | g3343.t59 | ProSitePatterns | PS00939 | Ribosomal protein L1e signature. | 73 | 99 | - |
| 5 | g3343.t59 | SUPERFAMILY | SSF52166 | Ribosomal protein L4 | 2 | 218 | 2.49E-71 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005840 | ribosome | CC |
| GO:0006412 | translation | BP |
| GO:0003735 | structural constituent of ribosome | MF |
KEGG
Orthology
- This transcript has been annotated with K02930 (large subunit ribosomal protein L4e)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
