General information for g3346.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein-serine O-palmitoleoyltransferase porcupine.
Parent gene
- The parent gene g3346 is located on chr_3: 24765762..24766514 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3346 | g3346.t1 | TSS | g3346.t1 | 24764662 | 24764662 |
| chr_3 | g3346 | g3346.t1 | isoform | g3346.t1 | 24764752 | 24766514 |
| chr_3 | g3346 | g3346.t1 | exon | g3346.t1.exon1 | 24764752 | 24764956 |
| chr_3 | g3346 | g3346.t1 | cds | g3346.t1.CDS1 | 24764752 | 24764956 |
| chr_3 | g3346 | g3346.t1 | exon | g3346.t1.exon2 | 24765068 | 24765269 |
| chr_3 | g3346 | g3346.t1 | cds | g3346.t1.CDS2 | 24765068 | 24765269 |
| chr_3 | g3346 | g3346.t1 | exon | g3346.t1.exon3 | 24765356 | 24765685 |
| chr_3 | g3346 | g3346.t1 | cds | g3346.t1.CDS3 | 24765356 | 24765685 |
| chr_3 | g3346 | g3346.t1 | exon | g3346.t1.exon4 | 24765762 | 24765988 |
| chr_3 | g3346 | g3346.t1 | cds | g3346.t1.CDS4 | 24765762 | 24765988 |
| chr_3 | g3346 | g3346.t1 | exon | g3346.t1.exon5 | 24766057 | 24766514 |
| chr_3 | g3346 | g3346.t1 | cds | g3346.t1.CDS5 | 24766057 | 24766514 |
| chr_3 | g3346 | g3346.t1 | TTS | g3346.t1 | 24766813 | 24766813 |
Sequences
>g3346.t1 Gene=g3346 Length=1422
ATGGCCTATAAGGGCTCATTTTATTATGATGATTACGATTTGGATCATTACGATGATTCA
TTTGAATATTCTTATGACGATCAATTGCCAAGTTTTGAATTAAGTGATATTTTAAGTTGT
GGCTCAACAGTATTAAAACAAATATTGAAGCAAATTTCATATTTGTTGGTGATAAACCTG
ATATACCGTGTGATAAGACAAACAGTCTCACTGCCGGAATTTCTCAAACATTTATCATCT
TCGCTACTTGGATGGTTATCAACATTTATATTTTTTTCGACTGGAAATTTTTATGTTCTT
TTATTAAATTTTGCATCATATGGGTTTCTCAAACTTTTACAAATTTATCAAGTTAAACGA
CAAGGACTAGCAACAATTGTTTTTCAAATAATTACAATTTTTACATGTGAAATAATTGAA
CGAGAAACAACAACATGGCAAAATTTACGTGGATCAGCGTTGATAATCTCAATGAAAGCA
ATCTCATTAGCTTATGACATCACATCAAATAAATTAAATAAATTACCTTCGATTTATGAA
TATACCGGCTACATGCTTTGTCCTGCAAATCTCGTGCTTGGGCCTTTTGTTTCATTTCTC
ACTTATAAACATATGGCAAAAGGAATGCGCTTTTCGATACGACACTTTCTTCATGTGCTA
GTGAATAGCTTTTTATCGATTGTGTTCATATTCCTATCTGCATGCTTCCTCAACTATCTT
ATTGCTGATGATGCCAGATTCATCGTGACATATAGAGATGCATTTATTTTTCGATGCAGT
CATTACTTTGTATCGTTCATGTCTGCTGCGGCATTATTGACGTCAGGTATAGAAAGTAAA
GATCGTGCATCGTCAGATATTTTTGGGTATCAAATCACACGACCACTTGATATCGAGTTA
CCTCGCTCGCTTGTACCTGTAGTGATTTCGTGGAACATTCCGATTCACTTGTGGATAAAA
AATTATGTCTTTGAGAATTTAAAGCGTTACGGAAAATTTAAAGCAATTGTTTTGACATAC
TTAATATCATCATCACTTCATGGATTACAAATTCAGTTGGCTGCAGTATTATTGTCGATT
GGAATTTTTTCGTATGTCGAATTTCGATTACGCAACTTACTTGCAGAAATTTTAGACGCC
TGTGTAAATGCAAACAAGTGCGTATTGGATGAAAAAGGTTGCTGCATCACGAAGGGACAT
GAATACACCACACGACATTTTTGGGTGAAGCTGATAAATTTTACATTTGGAATCTTCACA
GTAGTTCTCCTTGCCTATCTCGGTGTTCTACTCGACACATCCGTTGACAATCAAAATCAA
TTTTCATTTTATAGTAATTTAAAAAAATGGTATGATCTTAATTTCTTTGGCCATATAATT
GTTTTAATTTGGTACATGATGTACTTAATAGCAAAAAATTAA
>g3346.t1 Gene=g3346 Length=473
MAYKGSFYYDDYDLDHYDDSFEYSYDDQLPSFELSDILSCGSTVLKQILKQISYLLVINL
IYRVIRQTVSLPEFLKHLSSSLLGWLSTFIFFSTGNFYVLLLNFASYGFLKLLQIYQVKR
QGLATIVFQIITIFTCEIIERETTTWQNLRGSALIISMKAISLAYDITSNKLNKLPSIYE
YTGYMLCPANLVLGPFVSFLTYKHMAKGMRFSIRHFLHVLVNSFLSIVFIFLSACFLNYL
IADDARFIVTYRDAFIFRCSHYFVSFMSAAALLTSGIESKDRASSDIFGYQITRPLDIEL
PRSLVPVVISWNIPIHLWIKNYVFENLKRYGKFKAIVLTYLISSSLHGLQIQLAAVLLSI
GIFSYVEFRLRNLLAEILDACVNANKCVLDEKGCCITKGHEYTTRHFWVKLINFTFGIFT
VVLLAYLGVLLDTSVDNQNQFSFYSNLKKWYDLNFFGHIIVLIWYMMYLIAKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g3346.t1 | PANTHER | PTHR13906:SF12 | PROTEIN-SERINE O-PALMITOLEOYLTRANSFERASE PORCUPINE | 32 | 469 | 5.4E-104 |
| 3 | g3346.t1 | PANTHER | PTHR13906 | PORCUPINE | 32 | 469 | 5.4E-104 |
| 1 | g3346.t1 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 110 | 358 | 6.9E-13 |
| 12 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 47 | - |
| 30 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 48 | 65 | - |
| 21 | g3346.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 66 | 84 | - |
| 25 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 85 | 110 | - |
| 16 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 111 | 121 | - |
| 22 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 140 | - |
| 19 | g3346.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 141 | 180 | - |
| 24 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 181 | 202 | - |
| 14 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 203 | 222 | - |
| 28 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 223 | 242 | - |
| 17 | g3346.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 243 | 261 | - |
| 26 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 262 | 279 | - |
| 11 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 280 | 299 | - |
| 27 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 300 | 319 | - |
| 20 | g3346.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 320 | 338 | - |
| 23 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 339 | 366 | - |
| 15 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 367 | 410 | - |
| 29 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 411 | 431 | - |
| 18 | g3346.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 432 | 450 | - |
| 31 | g3346.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 451 | 471 | - |
| 13 | g3346.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 472 | 473 | - |
| 8 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 88 | 110 | - |
| 10 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 178 | 200 | - |
| 5 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 220 | 242 | - |
| 4 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 255 | 277 | - |
| 7 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 346 | 368 | - |
| 9 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 407 | 429 | - |
| 6 | g3346.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 449 | 471 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K00181 (O-palmitoleoyl transferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
