General information for g3346.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein-serine O-palmitoleoyltransferase porcupine.
Parent gene
- The parent gene g3346 is located on chr_3: 24765762..24766514 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3346 | g3346.t3 | isoform | g3346.t3 | 24763676 | 24766514 |
| chr_3 | g3346 | g3346.t3 | exon | g3346.t3.exon1 | 24763676 | 24764079 |
| chr_3 | g3346 | g3346.t3 | exon | g3346.t3.exon2 | 24765474 | 24765685 |
| chr_3 | g3346 | g3346.t3 | exon | g3346.t3.exon3 | 24765762 | 24766514 |
| chr_3 | g3346 | g3346.t3 | cds | g3346.t3.CDS1 | 24766035 | 24766514 |
| chr_3 | g3346 | g3346.t3 | TTS | g3346.t3 | 24766813 | 24766813 |
| chr_3 | g3346 | g3346.t3 | TSS | g3346.t3 | NA | NA |
Sequences
>g3346.t3 Gene=g3346 Length=1369
AATTATTAAGCTCTACAAATGGAGCATTTATTAGTTAGGCATCTTTCTTCCGAAAATGAA
ATGGAATAATATACATAAAAAATTAAGTGCGTTTCATCACTAATGCGGCAGCAGTCGGGT
GATATAAAAGAAAGAAATAGCATAATGATGAATTTTTATCGTGCTTTAAAATGAATTTCA
CATCACATCGCTTAATAGATAGATAGATGATTGCTCGCGCAAGATGTACCTTTGAGATTA
TGAGTTTGTGAGTGAGTGGAATGGTGGACCACTTGATGAATATAAAGTGACTATTCACTG
ATAATGATGATGATGGCTTTTGCTCAATACAATTTAACAATTTGAATAGTATTAAAAGGA
ACACACTGCTTTTCCAGTCCATAAAACGTTAATTATTACTTTCCCCTTCGATTTATGAAT
ATACCGGCTACATGCTTTGTCCTGCAAATCTCGTGCTTGGGCCTTTTGTTTCATTTCTCA
CTTATAAACATATGGCAAAAGGAATGCGCTTTTCGATACGACACTTTCTTCATGTGCTAG
TGAATAGCTTTTTATCGATTGTGTTCATATTCCTATCTGCATGCTTCCTCAACTATCTTA
TTGCTGATGATGCCAGATTCATCGTGACATATAGAGATGCATTTATTTTTCGATGCAGTC
ATTACTTTGTATCGTTCATGTCTGCTGCGGCATTATTGACGTCAGGTATAGAAAGTAAAG
ATCGTGCATCGTCAGATATTTTTGGGTATCAAATCACACGACCACTTGATATCGAGTTAC
CTCGCTCGCTTGTACCTGTAGTGATTTCGTGGAACATTCCGATTCACTTGTGGATAAAAA
ATTGTAAATTCACATTTCTTTCACAAAAAAATGTGCCATTTCTTAAAAAATGTCTTTCTT
TCATTTCACAGATGTCTTTGAGAATTTAAAGCGTTACGGAAAATTTAAAGCAATTGTTTT
GACATACTTAATATCATCATCACTTCATGGATTACAAATTCAGTTGGCTGCAGTATTATT
GTCGATTGGAATTTTTTCGTATGTCGAATTTCGATTACGCAACTTACTTGCAGAAATTTT
AGACGCCTGTGTAAATGCAAACAAGTGCGTATTGGATGAAAAAGGTTGCTGCATCACGAA
GGGACATGAATACACCACACGACATTTTTGGGTGAAGCTGATAAATTTTACATTTGGAAT
CTTCACAGTAGTTCTCCTTGCCTATCTCGGTGTTCTACTCGACACATCCGTTGACAATCA
AAATCAATTTTCATTTTATAGTAATTTAAAAAAATGGTATGATCTTAATTTCTTTGGCCA
TATAATTGTTTTAATTTGGTACATGATGTACTTAATAGCAAAAAATTAA
>g3346.t3 Gene=g3346 Length=159
MSFFHFTDVFENLKRYGKFKAIVLTYLISSSLHGLQIQLAAVLLSIGIFSYVEFRLRNLL
AEILDACVNANKCVLDEKGCCITKGHEYTTRHFWVKLINFTFGIFTVVLLAYLGVLLDTS
VDNQNQFSFYSNLKKWYDLNFFGHIIVLIWYMMYLIAKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 1 | g3346.t3 | PANTHER | PTHR13906:SF12 | PROTEIN-SERINE O-PALMITOLEOYLTRANSFERASE PORCUPINE | 7 | 155 | 1.5E-23 |
| 2 | g3346.t3 | PANTHER | PTHR13906 | PORCUPINE | 7 | 155 | 1.5E-23 |
| 8 | g3346.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 34 | - |
| 11 | g3346.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 35 | 54 | - |
| 7 | g3346.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 55 | 96 | - |
| 12 | g3346.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 97 | 117 | - |
| 9 | g3346.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 118 | 136 | - |
| 10 | g3346.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 137 | 157 | - |
| 6 | g3346.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 158 | 159 | - |
| 3 | g3346.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 32 | 54 | - |
| 4 | g3346.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 93 | 115 | - |
| 5 | g3346.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 135 | 157 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
