General information for g3385.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine/threonine-protein kinase polo.
Parent gene
- The parent gene g3385 is located on chr_3: 25014916..25017020 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3385 | g3385.t5 | TSS | g3385.t5 | 25011855 | 25011855 |
| chr_3 | g3385 | g3385.t5 | isoform | g3385.t5 | 25012175 | 25013587 |
| chr_3 | g3385 | g3385.t5 | exon | g3385.t5.exon1 | 25012175 | 25013587 |
| chr_3 | g3385 | g3385.t5 | cds | g3385.t5.CDS1 | 25013277 | 25013585 |
| chr_3 | g3385 | g3385.t5 | TTS | g3385.t5 | NA | NA |
Sequences
>g3385.t5 Gene=g3385 Length=1413
GCTAAAAGCCAATATAATTGATATTTCTACACAAATTCAATAACCTAAAAGAAATTCAAA
TTATTATGCATTAATCTAATGAGAATAAATTTTATTTCTTATTTTCTCATGTTAGGGAGG
ATTTGCAAAGTGTTATGAGATCCAGAATACTCAATCAAAAGAAACATATGCTGGCAAGAT
TGTCTCAAAAAAGCTCATGATGAAAAACAATCAAAAAGAAAAAATGACTCAAGAAATTCA
AATTCACAAGTCGTTAAATCACAAAAATATAGTTGGCTTTAAAAGTTTTTTTGACGATTC
AAATAATGTCTATATAGTTCTGGAACTCTGCAAGAAACGTTCAATGATGGAACTTCATAA
ACGAAGAAAAGTGGTTACTGATTATGAATGCCGTTACTACATTCATCAAATTGTAAGCGG
AGTAAAATATCTTCATGACCACCATATTATTCATCGCGATCTTAAACTTGGAAATCTCTT
TTTGAATGATGAATTACATGTTAAGTGAGTATATTGTATTTATATTGTATTTTATATTAT
AATGTATCTTTTTTAAAAGAATCGGTGACTTTGGTCTTGCTACTAAAATTGAATTCGAAG
GAGAAAGAAAGAAGACTTTATGTGGGACCCCAAATTATATTGCTCCTGAGATATTGAATA
AAAAAGGACATTCCTATGAGGTTGATATTTGGTCGATTGGCTGCGTTATGTATACGCTTC
TTGTTGGTCAACCTCCATTTGAGACAAAGAGCTTAAAAGATACATATTCAAAAATTCGAA
AGTGTGATTATAGATTGCCCTCGAACTTGCGCAAAAATGCTGCAGATATGATTATTGCTA
TGCTTCAATCTGATCCAGAAAAACGTCCAACAATTGATAAGCTATTTCATTTTGATTTCT
TATCATCTGCTCCAATTCCCAAGTCATTACCATATTCATGTTTGACAACAGCTCCTCGTC
CAGAATTACTTGAATATAATGATAAACGTCGCCCATTGTCGGAAATGAATGGTGGCGGTG
GTAAGTTATATATTTTTGTCATAAAATTCTTTTTCTTGAATTTATATATTGCTTTTTTTT
CATAGATGAAACTCACACAAATATGTCATTCTTGAAGAATAATTTGCATGATGCTATTAC
AGCATCTGGAATGATGACTGCTTCAAGTCAATTGCAGAAACAAAATTTGGAACTGCTGTA
TCAGCAACTGACTGAATTATTTGGAACAAAATTGAAACGCCATCTTGCTGATTTAGGTGA
TGAATACAGTGATCCAGCTTCTAATCCACTTTTCTGGGTCAGTAAATGGGTCGATTATAG
TGATAAATACGGTTTTGGATATCAATTATGTGACGAAGGAATGGGAGTTATGTTTAACGA
TACTACAAAATTACTCATGCTAAATAATGGAAT
>g3385.t5 Gene=g3385 Length=103
MSFLKNNLHDAITASGMMTASSQLQKQNLELLYQQLTELFGTKLKRHLADLGDEYSDPAS
NPLFWVSKWVDYSDKYGFGYQLCDEGMGVMFNDTTKLLMLNNG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g3385.t5 | CDD | cd13118 | POLO_box_1 | 62 | 103 | 0.000 |
| 5 | g3385.t5 | Gene3D | G3DSA:3.30.1120.30 | - | 18 | 103 | 0.000 |
| 2 | g3385.t5 | PANTHER | PTHR24345:SF86 | SERINE/THREONINE-PROTEIN KINASE PLK1 | 32 | 103 | 0.000 |
| 3 | g3385.t5 | PANTHER | PTHR24345 | SERINE/THREONINE-PROTEIN KINASE PLK | 32 | 103 | 0.000 |
| 1 | g3385.t5 | Pfam | PF00659 | POLO box duplicated region | 73 | 102 | 0.000 |
| 6 | g3385.t5 | ProSiteProfiles | PS50078 | POLO box domain profile. | 72 | 103 | 14.181 |
| 4 | g3385.t5 | SUPERFAMILY | SSF82615 | Polo-box domain | 29 | 102 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
