General information for g3424.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Receptor-type guanylate cyclase Gyc76C.
Parent gene
- The parent gene g3424 is located on chr_3: 25307298..25310029 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3424 | g3424.t2 | isoform | g3424.t2 | 25299575 | 25307298 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon1 | 25299575 | 25299737 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS1 | 25299575 | 25299737 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon2 | 25300439 | 25300987 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS2 | 25300439 | 25300987 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon3 | 25301052 | 25301129 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS3 | 25301052 | 25301129 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon4 | 25301202 | 25301374 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS4 | 25301202 | 25301374 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon5 | 25301509 | 25301580 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS5 | 25301509 | 25301580 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon6 | 25303193 | 25303338 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS6 | 25303193 | 25303338 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon7 | 25303408 | 25303579 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS7 | 25303408 | 25303579 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon8 | 25304328 | 25304783 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS8 | 25304328 | 25304783 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon9 | 25304848 | 25305024 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS9 | 25304848 | 25305024 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon10 | 25305085 | 25305346 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS10 | 25305085 | 25305346 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon11 | 25305408 | 25305531 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS11 | 25305408 | 25305531 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon12 | 25305610 | 25306215 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS12 | 25305610 | 25306215 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon13 | 25306273 | 25306412 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS13 | 25306273 | 25306412 |
| chr_3 | g3424 | g3424.t2 | exon | g3424.t2.exon14 | 25306475 | 25307298 |
| chr_3 | g3424 | g3424.t2 | cds | g3424.t2.CDS14 | 25306475 | 25307298 |
| chr_3 | g3424 | g3424.t2 | TSS | g3424.t2 | NA | NA |
| chr_3 | g3424 | g3424.t2 | TTS | g3424.t2 | NA | NA |
Sequences
>g3424.t2 Gene=g3424 Length=3942
ATGAAGTGGAAGTTGCTCACAATCAATCAAAAGTTTGTGTTTTTGTTATTTAATTGTTTT
CTACTTATCAACTTTATCTCTATTGTCAATTGTGATGTTTTTAAACTTGGCTATATAACT
GGCTCACATCGTCGTCATGGAGATTTGGAATATGAGCGTCCTGGTTTAACTATATCGGGT
GCACTCACGCTTGCAGTCACGGAAGTAAATAATGAGAAGCTTGCCAAATTGAATCATTCG
ATTGAATTCATTGTTGCTGAAACTTATGGCGAAGAGATATCGAGTATTCGCGAGACGGCG
AGCTTATGGAAGCAAGGAATTTATGGCTATATTGGACCACAAGAGACATGCATTCACGAA
GGTCGGATGGCGGCAGCATTCAATCTACCAATGATTTCTTACTTTTGTACCAGCTATGAA
ACATCAAATAAAAAAGATTTCACCACATTCGCACGAACTCGTCCACCTGACACGCAAATT
GCAAAATCAGTTGTTAGTCTTCTCCTTGCATATAATTGGACGCAAGTGACATTTTTGCAT
GTCAAGCATGAAAGTAGTGCAATTGCACCAATAGCAAAGACTGTGATAAATACATTGAAT
CTAGCGGGAATAAAAATAAATGCGGTAAGAACTTGGAATGAAGACTATTTTCATGGTTAT
CGTGCGAATCCATTTGATGATTTGGTGCGTGACACATACAGGGATACTAGAATTTACTTA
ATTTTGGGACAATTTCATGAGCACATTGGATTAATGATGTCTCTCTATAGTCGAGGACTT
TTGAATGATGGCGAATATTTTGTTGTTGGTATGGACATTGAACAATATGATTCCAGTGAC
CCAGAAAAATACTTGAAAGGACTTTTGGCAAGTGAGCAAGAATCGTGGGTTGGTGACGCT
TATAAATCATATCTTGCTGTCTTACCATCACCTACAATAGGCTTTGATGATTTTGCAACA
AAAGTAAATAAATTCCTCGAGTTGCCGCCATTTGAACATCCCAATCCGCTGAAACCATTC
AACAAAACGAAACAGATACGTGCGGAAGCTGCCTATCTCTATGATGCTGTTCATCTCTAT
GCAGATGCTTTAATTAATTGTCTTGAGAACCATGGGGATCCAAAGAATGGTAGCGAAATA
ATCAATGCTATCAAAGGACGAAGCTATCATAGTGCTATGGGTTTTTTAGTATATATAGAT
GAGAATGGTGATGCTGGAGGTAATTACACAATTGTCGGAATGAAGGCAATGAACAATGAC
AAGTCAAATCGCACCACTTATGGTCTCTTTCCTATTGGAACTTTCATCACAAGAATAAAT
CAAAATGAAAGTATTAAAAGCAACAGTATACCGGATTTACAACTATTCGAGCGTTTATCA
TGGGTAGGTGGCAAAGTGCCAATTGCAGAGCCAATATGTGGTTTCCGAGGCGAAAAGTGT
ATCAGTTACGCAAGAGAAATTACCGCAGCAATAGCCGGCTTCATTCTTCTTATTCTGACT
ATCGTTTCACTCGTTCTTTATCGTAATTGGCGTTACGAGCAAGAACTTGATAGTCTCTTG
TGGAAAATTGACTTCAAAGAAATTCAAATGCATGAGAATGAGACAAATGGCACTCAAAAG
CAGACACGAGCTACACATCCACTCATTCGAACGAGTCAAGTGAGTTTGAGCTCTAATCCT
GACGCAGACTTCCGATATTCGACAATATTCACTCCAATCGGTTTGTATAAAGGACAACTG
TATGCTATAAAAAAGATAAGAAAAAAGTCAGTTGACATCACACGAGAGATGAAGATGGAA
TTGAAAATGTTACGCGATATTCGACATGATAATCTTAATGCATTCATTGGAGCTTGTACA
GAACCACCAAACATTTGCATTATAACTGAATACTGTTCTCGTGGGAGTCTCAAAGATGTT
TTAGAAAATGATGATGTAAAATTGGACAATATGTTCATTGCATCATTAGTTTTTGATCTT
ATAAGAGGCATGACCTACCTTCATGATTCACCATTGCGTGTTCATGGCAACTTATGTACA
TCAAATTGTCTCATTGATTCACGTTGGGTTGTAAAACTGAGTGATTTTGGTTTGTATGCA
TTTAAGAATGGCGGAAACAATCATCATCACAGCTGTGACGGCGGGAAACACAATTTGAGT
GAAAATAATGAAATTTATGAAGAAGACATGATGATACCCATTAGACGTGGCAGTACTGAT
TCGAGAAAATTTTGCGCCAAATATGAAAAACTTCTTTATAAAGCACCAGAGTTACTAAGA
ACTGTCAGTAATTTTAACACAATGTTAATACCTGGCTCACAAAAGGGCGACGTTTATTCA
TTTGCAATAATCTTATATGAAATCCATACAAGAAATGGACCATTTGGAGAAATTGACTGC
ACTTATCTCGATTGCCTGAAGCGTCTTACTGTTCCATTAGACATTACAAATCCTTTCCGA
CCTTCTCTACAGCCGCTTGAGACTTCCTTTGATTGTGTACGTGAATGCATAAGAGAATGC
TGGTGCGAAAATCCTGAAGCACGACCCGACTTTAAATCAATCCGAGCAAAATTGCGTCCA
TTGCGAAAGGGCATGAGACCAAATATCTTTGACAATATGATGGCAATGATGGAAAAGTAT
GCCAACAATTTAGAACAGCTTGTTGATGAAAGAACGGATCAGTTACAGGAAGAGAAAAAG
AAGACGGAAGCATTGCTGCTGGAGATGCTTCCACGACCCGTAGCAGAGCAACTGAAGCGT
GGAAATAAGGTCGAAGCTGAAAGTTATGACATGGTCACCATTTATTTCTCTGATATTGTC
GGATTCACGTCAATGAGCGCTGAAAGCACACCACTACAAGTTGTCGATTTTCTCAATGAT
CTCTATACATGTTTTGATTCAATCATTGGCAATTATGATGTTTATAAAGTAGAAACTATT
GGTGATGCCTATATGGTTTGCTCAGGACTTCCTATAAGAAATGGTGACATTCATGCAGGT
GAAATTGCCAGTATGTCACTACATTTATTGGAAGCAATTCGTGAATTTAAAATTAGACAT
CGACCAAATGACACATTGAAGCTAAGAATAGGCATTCATAGTGGTCCTGTTTGTGCTGGT
GTTGTTGGATTAAAAATGCCAAGATTTTGTCTATTTGGTGATACTGTCAATACATCATCA
AGAATGGAATCAACAGGTGAAGCATTGAAAATTCATTGTAGTGACACATGCAAGAAACTT
CTTGATAGTCTTGGCGGATATATTTTTGAAGATCGTGGAAAAATTCCAATTAAAGGCAAA
GGTGAACTCAATACTTATTGGCTTGTTGGTGAACTTGAAGATTATAAACTTAAACGATAT
CAAGCAAGAACTGAAAGAAGAGCATCAAAAAATAGCGCAAAACAAATTTCAAAGTTTCTT
CAAGCACAAAAAGAATTACAAAAAAGCTCTTTAAAGAGTACAAAAAGTTTTTTGGCAAAT
AGTCCAAATCGTCTCACACCATTTGCCAACAATCTTATGCGATCAACAAGTTTTGATTCA
CCAAAGAAACTTCGATTTGCTGCTGATATTCTTGATGCTCCTCAATATAGTAGCAACAAG
TATTTGCGTTATAGCAATGATGCATTAATGGAAGTTATCAGTGATCAAATAACGAGCATA
ATGAAAGTAAACAAAAATTCTGATGATTCATTAATTTTTGCTGTTGTTGATGCAAATGAG
AAGCCAAGAAGTAGTGATGGCTGTGATGACCTTACAGCATCATGTCCATGCATTAACAAG
GAAATCACTGAATTTATTTGTAATGGCGAGGACTTTTTTGAGGAAATGCCGCATGATGAC
GATATTGAATCAGACATTCATGATGTTTTACTTTTGGGAAACATAAAAACTACACCAACA
ATAAAAATTACAAGCAGCACACCGTTATTGTCACAAAACTGA
>g3424.t2 Gene=g3424 Length=1313
MKWKLLTINQKFVFLLFNCFLLINFISIVNCDVFKLGYITGSHRRHGDLEYERPGLTISG
ALTLAVTEVNNEKLAKLNHSIEFIVAETYGEEISSIRETASLWKQGIYGYIGPQETCIHE
GRMAAAFNLPMISYFCTSYETSNKKDFTTFARTRPPDTQIAKSVVSLLLAYNWTQVTFLH
VKHESSAIAPIAKTVINTLNLAGIKINAVRTWNEDYFHGYRANPFDDLVRDTYRDTRIYL
ILGQFHEHIGLMMSLYSRGLLNDGEYFVVGMDIEQYDSSDPEKYLKGLLASEQESWVGDA
YKSYLAVLPSPTIGFDDFATKVNKFLELPPFEHPNPLKPFNKTKQIRAEAAYLYDAVHLY
ADALINCLENHGDPKNGSEIINAIKGRSYHSAMGFLVYIDENGDAGGNYTIVGMKAMNND
KSNRTTYGLFPIGTFITRINQNESIKSNSIPDLQLFERLSWVGGKVPIAEPICGFRGEKC
ISYAREITAAIAGFILLILTIVSLVLYRNWRYEQELDSLLWKIDFKEIQMHENETNGTQK
QTRATHPLIRTSQVSLSSNPDADFRYSTIFTPIGLYKGQLYAIKKIRKKSVDITREMKME
LKMLRDIRHDNLNAFIGACTEPPNICIITEYCSRGSLKDVLENDDVKLDNMFIASLVFDL
IRGMTYLHDSPLRVHGNLCTSNCLIDSRWVVKLSDFGLYAFKNGGNNHHHSCDGGKHNLS
ENNEIYEEDMMIPIRRGSTDSRKFCAKYEKLLYKAPELLRTVSNFNTMLIPGSQKGDVYS
FAIILYEIHTRNGPFGEIDCTYLDCLKRLTVPLDITNPFRPSLQPLETSFDCVRECIREC
WCENPEARPDFKSIRAKLRPLRKGMRPNIFDNMMAMMEKYANNLEQLVDERTDQLQEEKK
KTEALLLEMLPRPVAEQLKRGNKVEAESYDMVTIYFSDIVGFTSMSAESTPLQVVDFLND
LYTCFDSIIGNYDVYKVETIGDAYMVCSGLPIRNGDIHAGEIASMSLHLLEAIREFKIRH
RPNDTLKLRIGIHSGPVCAGVVGLKMPRFCLFGDTVNTSSRMESTGEALKIHCSDTCKKL
LDSLGGYIFEDRGKIPIKGKGELNTYWLVGELEDYKLKRYQARTERRASKNSAKQISKFL
QAQKELQKSSLKSTKSFLANSPNRLTPFANNLMRSTSFDSPKKLRFAADILDAPQYSSNK
YLRYSNDALMEVISDQITSIMKVNKNSDDSLIFAVVDANEKPRSSDGCDDLTASCPCINK
EITEFICNGEDFFEEMPHDDDIESDIHDVLLLGNIKTTPTIKITSSTPLLSQN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 23 | g3424.t2 | CDD | cd06370 | PBP1_SAP_GC-like | 34 | 435 | 1.4956E-163 |
| 24 | g3424.t2 | CDD | cd14042 | PK_GC-A_B | 549 | 864 | 2.61876E-135 |
| 25 | g3424.t2 | CDD | cd07302 | CHD | 932 | 1108 | 1.00107E-65 |
| 15 | g3424.t2 | Coils | Coil | Coil | 870 | 901 | - |
| 14 | g3424.t2 | Gene3D | G3DSA:3.40.50.2300 | - | 58 | 409 | 1.6E-68 |
| 13 | g3424.t2 | Gene3D | G3DSA:3.40.50.2300 | - | 159 | 475 | 1.6E-68 |
| 12 | g3424.t2 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 570 | 898 | 6.1E-56 |
| 11 | g3424.t2 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 919 | 1116 | 7.4E-75 |
| 4 | g3424.t2 | PANTHER | PTHR11920:SF475 | GUANYLATE CYCLASE | 29 | 709 | 0.0 |
| 6 | g3424.t2 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 29 | 709 | 0.0 |
| 5 | g3424.t2 | PANTHER | PTHR11920:SF475 | GUANYLATE CYCLASE | 737 | 1270 | 0.0 |
| 7 | g3424.t2 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 737 | 1270 | 0.0 |
| 2 | g3424.t2 | Pfam | PF01094 | Receptor family ligand binding region | 60 | 416 | 1.7E-38 |
| 3 | g3424.t2 | Pfam | PF00069 | Protein kinase domain | 567 | 854 | 1.7E-28 |
| 1 | g3424.t2 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 924 | 1109 | 4.8E-64 |
| 18 | g3424.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 31 | - |
| 19 | g3424.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 11 | - |
| 20 | g3424.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 12 | 23 | - |
| 22 | g3424.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 24 | 31 | - |
| 17 | g3424.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 32 | 486 | - |
| 21 | g3424.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 487 | 507 | - |
| 16 | g3424.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 508 | 1313 | - |
| 29 | g3424.t2 | ProSitePatterns | PS00452 | Guanylate cyclase signature. | 1040 | 1063 | - |
| 31 | g3424.t2 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 525 | 862 | 26.803 |
| 30 | g3424.t2 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 933 | 1063 | 50.796 |
| 28 | g3424.t2 | SMART | SM00220 | serkin_6 | 566 | 862 | 1.9E-13 |
| 27 | g3424.t2 | SMART | SM00044 | cyc_6 | 897 | 1091 | 2.2E-104 |
| 8 | g3424.t2 | SUPERFAMILY | SSF53822 | Periplasmic binding protein-like I | 33 | 475 | 1.28E-70 |
| 9 | g3424.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 575 | 899 | 3.56E-50 |
| 10 | g3424.t2 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 918 | 1109 | 6.12E-64 |
| 26 | g3424.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 34 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016849 | phosphorus-oxygen lyase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
