Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Serine protease 3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3425 g3425.t15 TTS g3425.t15 25307620 25307620
chr_3 g3425 g3425.t15 isoform g3425.t15 25307703 25308886
chr_3 g3425 g3425.t15 exon g3425.t15.exon1 25307703 25307782
chr_3 g3425 g3425.t15 cds g3425.t15.CDS1 25307703 25307782
chr_3 g3425 g3425.t15 exon g3425.t15.exon2 25307873 25307962
chr_3 g3425 g3425.t15 cds g3425.t15.CDS2 25307873 25307962
chr_3 g3425 g3425.t15 exon g3425.t15.exon3 25308036 25308327
chr_3 g3425 g3425.t15 cds g3425.t15.CDS3 25308036 25308327
chr_3 g3425 g3425.t15 exon g3425.t15.exon4 25308384 25308504
chr_3 g3425 g3425.t15 cds g3425.t15.CDS4 25308384 25308504
chr_3 g3425 g3425.t15 exon g3425.t15.exon5 25308573 25308775
chr_3 g3425 g3425.t15 cds g3425.t15.CDS5 25308573 25308775
chr_3 g3425 g3425.t15 exon g3425.t15.exon6 25308842 25308886
chr_3 g3425 g3425.t15 cds g3425.t15.CDS6 25308842 25308886
chr_3 g3425 g3425.t15 TSS g3425.t15 25308935 25308935

Sequences

>g3425.t15 Gene=g3425 Length=831
ATGGAAAAGTTTTTACTTTTATTAACAATTATTTTATTTTTTGCTGCTGGTGTAACACAT
GTGCTACCTAATCAATTGCCATTATTCGAAAGAAAAGTGCAAGGAAGAATTGTCGGTGGT
TCGCGACCAAGTACAACTCAATTTCCCTATACAGTGTCAGTTCGCGCATTTAATGGAACA
TCAACCTCGTTTTGCGGTGGTTCTATAATTTCTTACAATTTTGTTCTGACTGCTGCACAT
TGCACTCGTGGCTATCAAGATTTCGAAATTGGTGTAGGCTCAATAGTTCTTCAAACTCCA
TTTTTTAAAATACTAACTTATGGTGGAGCAATAGAACATCCATATTTTAATCCAACAAAT
TTAAATAATGACATTTCACTCATAGAAGTTCCAACTATTGCCCAAAATGTTCCGTCTGTT
GTTCCAGTTTTACTTCCTAAAAGATCACAAGCCGATAAACCTTTTATTGGAATGCAAGTG
ACAGTATCAGGATTTGGAAGAACATCGGACGCATCACAAAGTGTTTCACCATTCATGGAA
TATGTTCAACTAAAAATTATCTCTAATAAAGATTGTGCAGGAACATATGGTAGAAGAATT
GTTACTGATGATGTTATTTGTGCTAAGGGAATTGATAAAAACTTTAATGCTTGTATTGGA
GATTCAGGAGGTCCTTTAACAATGAAAGAAGCAGGAAATCATGTTCAACTTGGTATTGTG
TCTTTTGTTAGTTCAAGAGGCTGTGCTATTGGTGATCCTTCAGGTTATACAAAAGTAGGA
AAATATCTCGATTGGATTTCTGCCAAAACTGGAATAATAATTCGCAATTAA

>g3425.t15 Gene=g3425 Length=276
MEKFLLLLTIILFFAAGVTHVLPNQLPLFERKVQGRIVGGSRPSTTQFPYTVSVRAFNGT
STSFCGGSIISYNFVLTAAHCTRGYQDFEIGVGSIVLQTPFFKILTYGGAIEHPYFNPTN
LNNDISLIEVPTIAQNVPSVVPVLLPKRSQADKPFIGMQVTVSGFGRTSDASQSVSPFME
YVQLKIISNKDCAGTYGRRIVTDDVICAKGIDKNFNACIGDSGGPLTMKEAGNHVQLGIV
SFVSSRGCAIGDPSGYTKVGKYLDWISAKTGIIIRN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g3425.t15 CDD cd00190 Tryp_SPc 37 267 3.63192E-61
9 g3425.t15 Gene3D G3DSA:2.40.10.10 - 29 271 5.7E-57
2 g3425.t15 PANTHER PTHR24260 - 31 272 3.7E-62
3 g3425.t15 PANTHER PTHR24260:SF90 AT07769P-RELATED 31 272 3.7E-62
5 g3425.t15 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 66 81 1.4E-11
6 g3425.t15 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 120 134 1.4E-11
4 g3425.t15 PRINTS PR00722 Chymotrypsin serine protease family (S1) signature 215 227 1.4E-11
1 g3425.t15 Pfam PF00089 Trypsin 37 266 5.6E-46
11 g3425.t15 Phobius SIGNAL_PEPTIDE Signal peptide region 1 23 -
12 g3425.t15 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 3 -
13 g3425.t15 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 4 15 -
14 g3425.t15 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 16 23 -
10 g3425.t15 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 24 276 -
18 g3425.t15 ProSitePatterns PS00134 Serine proteases, trypsin family, histidine active site. 76 81 -
19 g3425.t15 ProSitePatterns PS00135 Serine proteases, trypsin family, serine active site. 216 227 -
20 g3425.t15 ProSiteProfiles PS50240 Serine proteases, trypsin domain profile. 37 271 26.769
17 g3425.t15 SMART SM00020 trypsin_2 36 266 5.0E-54
7 g3425.t15 SUPERFAMILY SSF50494 Trypsin-like serine proteases 3 271 7.21E-65
8 g3425.t15 SignalP_EUK SignalP-noTM SignalP-noTM 1 23 -
16 g3425.t15 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 5 22 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004252 serine-type endopeptidase activity MF
GO:0006508 proteolysis BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed