Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Chymotrypsin BI.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g3425 g3425.t3 TTS g3425.t3 25307620 25307620
chr_3 g3425 g3425.t3 isoform g3425.t3 25307703 25308506
chr_3 g3425 g3425.t3 exon g3425.t3.exon1 25307703 25307782
chr_3 g3425 g3425.t3 cds g3425.t3.CDS1 25307703 25307782
chr_3 g3425 g3425.t3 exon g3425.t3.exon2 25307873 25307962
chr_3 g3425 g3425.t3 cds g3425.t3.CDS2 25307873 25307962
chr_3 g3425 g3425.t3 exon g3425.t3.exon3 25308036 25308327
chr_3 g3425 g3425.t3 cds g3425.t3.CDS3 25308036 25308225
chr_3 g3425 g3425.t3 exon g3425.t3.exon4 25308384 25308506
chr_3 g3425 g3425.t3 TSS g3425.t3 25308935 25308935

Sequences

>g3425.t3 Gene=g3425 Length=585
AGTGGCTATCAAGATTTCGAAATTGGTGTAGGCTCAATAGTTCTTCAAACTCCATTTTTT
AAAATACTAACTTATGGTGGAGCAATAGAACATCCATATTTTAATCCAACAAATTTAAAT
AATGACATTTCACTCATAGAAGTTCCAACTATTGCCCAAAATGTTCCGTCTGTTGTTCCA
GTTTTACTTCCTAAAAGATCACAAGCCGATAAACCTTTTATTGGAATGCAAGTGACAGTA
TCAGGATTTGGAAGAACATCGGACGCATCACAAAGTGTTTCACCATTCATGGAATATGTT
CAACTAAAAATTATCTCTAATAAAGATTGTGCAGGAACATATGGTAGAAGAATTGTTACT
GATGATGTTATTTGTGCTAAGGGAATTGATAAAAACTTTAATGCTTGTATTGGAGATTCA
GGAGGTCCTTTAACAATGAAAGAAGCAGGAAATCATGTTCAACTTGGTATTGTGTCTTTT
GTTAGTTCAAGAGGCTGTGCTATTGGTGATCCTTCAGGTTATACAAAAGTAGGAAAATAT
CTCGATTGGATTTCTGCCAAAACTGGAATAATAATTCGCAATTAA

>g3425.t3 Gene=g3425 Length=119
MQVTVSGFGRTSDASQSVSPFMEYVQLKIISNKDCAGTYGRRIVTDDVICAKGIDKNFNA
CIGDSGGPLTMKEAGNHVQLGIVSFVSSRGCAIGDPSGYTKVGKYLDWISAKTGIIIRN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g3425.t3 Gene3D G3DSA:2.40.10.10 - 1 111 8.6E-29
2 g3425.t3 PANTHER PTHR24250:SF56 SERINE PROTEASE P96 3 113 4.8E-33
3 g3425.t3 PANTHER PTHR24250 CHYMOTRYPSIN-RELATED 3 113 4.8E-33
1 g3425.t3 Pfam PF00089 Trypsin 2 109 6.6E-21
5 g3425.t3 ProSitePatterns PS00135 Serine proteases, trypsin family, serine active site. 59 70 -
7 g3425.t3 ProSiteProfiles PS50240 Serine proteases, trypsin domain profile. 1 114 14.518
4 g3425.t3 SUPERFAMILY SSF50494 Trypsin-like serine proteases 2 114 1.45E-31

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004252 serine-type endopeptidase activity MF
GO:0006508 proteolysis BP

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed