General information for g350.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Apyrase.
Parent gene
- The parent gene g350 is located on chr_3: 2815117..2816672 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g350 | g350.t1 | isoform | g350.t1 | 2815117 | 2816672 |
| chr_3 | g350 | g350.t1 | exon | g350.t1.exon1 | 2815117 | 2815454 |
| chr_3 | g350 | g350.t1 | cds | g350.t1.CDS1 | 2815117 | 2815454 |
| chr_3 | g350 | g350.t1 | exon | g350.t1.exon2 | 2815670 | 2816411 |
| chr_3 | g350 | g350.t1 | cds | g350.t1.CDS2 | 2815670 | 2816411 |
| chr_3 | g350 | g350.t1 | exon | g350.t1.exon3 | 2816469 | 2816672 |
| chr_3 | g350 | g350.t1 | cds | g350.t1.CDS3 | 2816469 | 2816672 |
| chr_3 | g350 | g350.t1 | TSS | g350.t1 | 2816835 | 2816835 |
| chr_3 | g350 | g350.t1 | TTS | g350.t1 | NA | NA |
Sequences
>g350.t1 Gene=g350 Length=1284
ATGATGGCAAACACATGTAAAGATCCGAATGAGTGTATTGGAGGATACGCGAGAGTAGTT
TCGACAGTTAGAAAACTCAAATCATCACTGCCAAATCCAATATATTTAAATGTTGCCGAT
AATTTTCAAGGTACATTTTGGTATAATAAGTTTCGATGGAATGCCACACAATATTTCCTC
GATTTAGAACCAGCTGATGCTATGACAATTGGCAATCATGAGTTCGATCACGGTATTGAA
GGATTAGTGCCATTCTTAGAGAGAATTAAATCGCCAGTTATCAGTTGTAATATTAATACC
ACACATGAACCAGAATTTGGAAAACTCTTCAATAAGTCAATGATTATTGAGAGAAATGGA
AGACAAATCGGTATAGTTGGTGTCACAACGACTTTCCCATCAGGTTGGGGAAGAGCAGTA
GTTTTGCCTGAAGTCGAGAATGTTAAAAAAGAAGTTGACAATTTAGTCGAGAAAGGAATA
AAAATTATCATCGTTCTCTCACATTCTGGCCTTGAAGTTGACCGTGAGATTGCAAAACAT
GGTGGAGATATTGATATAATTGTCGGTGGTCATTCTCATTCATTTCTTTACTCTGGAACT
CCTTCAATTGGACCAGACACACCTGTAAGTGACTATCCAACAATTGAAGAACAAGAAAAC
GGTAGAAAAGTTCTAATAGTTCAAGCATCTGCTTATGCAAAATATCTTGGAAATATCACA
CTCTATTTTGATGAAGATGGTGAAATTAAAAACTATGAAGGTGCACCAATATTTTTGTCA
CATGATATCGAGCAAGATACTGAAGTTGTAGAAAAGTTAAAACCATGGAAGAAAGAACTT
GATGCGATTCAAAATAAATATATTGGTTCAATTAGACATCAGCTTGATGCTTTTTGTTAC
AGAAAAGAATGTGGCATGGGAAATCTTGTAACAGATTCAATGACTTTGGAAATACCTGGT
GAAGGCATTAGAGAAGCATTGGAATATTCTGTTTCTGATGCTGAAAATCTTCATGTAATG
CAAGTGTCAGGCATTAAAGTTATTTATGATATGACTCGAAAGCCATATGATCGAATTGTT
GATCTTAAAGTTCTTTGTCAAGCTTGTGAGATTCCAAAATATGAAGATCTTAAAGATACT
AAAATTTACAAAGTGGTGTTAGCTGAATATATTTATGCTGGTGGTGATAGTTACAAAATG
TTTCCAAATATGCTAAAAATCCAATTGAAGGCCCAAGAGATGTTGATGCACTTGCAGATT
ACATTCAGAAATATCTCCAATTAA
>g350.t1 Gene=g350 Length=427
MMANTCKDPNECIGGYARVVSTVRKLKSSLPNPIYLNVADNFQGTFWYNKFRWNATQYFL
DLEPADAMTIGNHEFDHGIEGLVPFLERIKSPVISCNINTTHEPEFGKLFNKSMIIERNG
RQIGIVGVTTTFPSGWGRAVVLPEVENVKKEVDNLVEKGIKIIIVLSHSGLEVDREIAKH
GGDIDIIVGGHSHSFLYSGTPSIGPDTPVSDYPTIEEQENGRKVLIVQASAYAKYLGNIT
LYFDEDGEIKNYEGAPIFLSHDIEQDTEVVEKLKPWKKELDAIQNKYIGSIRHQLDAFCY
RKECGMGNLVTDSMTLEIPGEGIREALEYSVSDAENLHVMQVSGIKVIYDMTRKPYDRIV
DLKVLCQACEIPKYEDLKDTKIYKVVLAEYIYAGGDSYKMFPNMLKIQLKAQEMLMHLQI
TFRNISN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g350.t1 | CDD | cd07409 | MPP_CD73_N | 3 | 259 | 0 |
| 12 | g350.t1 | Gene3D | G3DSA:3.60.21.10 | - | 2 | 310 | 0 |
| 13 | g350.t1 | Gene3D | G3DSA:3.90.780.10 | - | 313 | 426 | 0 |
| 3 | g350.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 3 | 315 | 0 |
| 5 | g350.t1 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 3 | 315 | 0 |
| 4 | g350.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 315 | 406 | 0 |
| 6 | g350.t1 | PANTHER | PTHR11575:SF32 | APYRASE-LIKE PROTEIN | 315 | 406 | 0 |
| 9 | g350.t1 | PRINTS | PR01607 | Apyrase family signature | 158 | 175 | 0 |
| 8 | g350.t1 | PRINTS | PR01607 | Apyrase family signature | 177 | 200 | 0 |
| 7 | g350.t1 | PRINTS | PR01607 | Apyrase family signature | 224 | 244 | 0 |
| 2 | g350.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 11 | 194 | 0 |
| 1 | g350.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 314 | 402 | 0 |
| 11 | g350.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 8 | 281 | 0 |
| 10 | g350.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 286 | 406 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
