General information for g3557.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative OTU domain-containing protein 5-B.
Parent gene
- The parent gene g3557 is located on chr_3: 26425801..26426126 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3557 | g3557.t8 | TSS | g3557.t8 | 26422154 | 26422154 |
| chr_3 | g3557 | g3557.t8 | isoform | g3557.t8 | 26422301 | 26425222 |
| chr_3 | g3557 | g3557.t8 | exon | g3557.t8.exon1 | 26422301 | 26422351 |
| chr_3 | g3557 | g3557.t8 | cds | g3557.t8.CDS1 | 26422301 | 26422351 |
| chr_3 | g3557 | g3557.t8 | exon | g3557.t8.exon2 | 26422757 | 26422839 |
| chr_3 | g3557 | g3557.t8 | cds | g3557.t8.CDS2 | 26422757 | 26422839 |
| chr_3 | g3557 | g3557.t8 | exon | g3557.t8.exon3 | 26424345 | 26424997 |
| chr_3 | g3557 | g3557.t8 | cds | g3557.t8.CDS3 | 26424345 | 26424997 |
| chr_3 | g3557 | g3557.t8 | exon | g3557.t8.exon4 | 26425061 | 26425222 |
| chr_3 | g3557 | g3557.t8 | cds | g3557.t8.CDS4 | 26425061 | 26425221 |
| chr_3 | g3557 | g3557.t8 | TTS | g3557.t8 | 26426198 | 26426198 |
Sequences
>g3557.t8 Gene=g3557 Length=949
ATGACTATTTTGCCAATATTAAATAAGAAATCTGGAAATGAAGAAAAAGATAATGTACAG
AGAGACAAAAGAATAAATCCTGATAGATTAAACTATGAAGTTAGACGTAGTCCAACGAGT
CGTAGTAGTAAAAGTCATGGGCGCTCCAGTAAACGTGAAAAGGCGGCAAATGGGGTACAA
GTTCAAACAAAATATCATCAGCCTCATGCCTCATCATCATCTTCCACTGCAGCAACGAGT
GTAAATAAAAAGCAACAACTGCCTTGTCCAACAAACGTTGTATCATGCGTCACACAGCAG
CAAAGTCAACTGCACAGTGTCAGCACATCGACTGCCGTTGCTGAATGTTCTAAAAGTGAT
AACAATATAGCTGGCAATAGCTGTGTGAACAAGAAAATCAATGATCAAGAATGTAATGAT
GACTTTTTAAAAATTCTCGAAGAAAAAGTATCTGGCTATAATAGCGGTGATGAGCATTTG
GAATCAAAAGAAAATCACTTGACTAATGATGAATGGAAAAGTCGTGATGAAAAATTTTCA
ACACTACTTGAATCTGAACGAGGTTTGATAATTAAAAACATGGAAGAAGATGGTGCATGC
CTTTTTAGGGCAATATCTTATCAAATTTACGGTGATCAAGACATGCACGATATCATAAGA
CAACAAACTATGGATTATATCTATCAGAATCGTGAATATTTTGAACAATTTCTAACTGAG
GACATTCACTCATATGTTAAACGTAAAAGACAAAACCATCTGCATGGCAACCATATAGAA
ATTCAAGCAATGTCAGAAATGTACAATAGACCCGTCGAATTGTATTGTTATGAGCTGGCG
CCTTTAAATATATATAATCCAGAACAAATTAATAATGGTTACGATCCTTTACGACTATCA
TACCAGAGGAATTGCCATTATAATGCGATATTAGATCCTTATAAGTCAT
>g3557.t8 Gene=g3557 Length=316
MTILPILNKKSGNEEKDNVQRDKRINPDRLNYEVRRSPTSRSSKSHGRSSKREKAANGVQ
VQTKYHQPHASSSSSTAATSVNKKQQLPCPTNVVSCVTQQQSQLHSVSTSTAVAECSKSD
NNIAGNSCVNKKINDQECNDDFLKILEEKVSGYNSGDEHLESKENHLTNDEWKSRDEKFS
TLLESERGLIIKNMEEDGACLFRAISYQIYGDQDMHDIIRQQTMDYIYQNREYFEQFLTE
DIHSYVKRKRQNHLHGNHIEIQAMSEMYNRPVELYCYELAPLNIYNPEQINNGYDPLRLS
YQRNCHYNAILDPYKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g3557.t8 | Gene3D | G3DSA:3.90.70.80 | - | 169 | 316 | 6.5E-52 |
| 7 | g3557.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 83 | - |
| 5 | g3557.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 10 | 35 | - |
| 6 | g3557.t8 | MobiDBLite | mobidb-lite | consensus disorder prediction | 55 | 83 | - |
| 2 | g3557.t8 | PANTHER | PTHR12419:SF4 | OTU DOMAIN-CONTAINING PROTEIN 5 | 26 | 315 | 2.6E-69 |
| 3 | g3557.t8 | PANTHER | PTHR12419 | OTU DOMAIN CONTAINING PROTEIN | 26 | 315 | 2.6E-69 |
| 1 | g3557.t8 | Pfam | PF02338 | OTU-like cysteine protease | 197 | 285 | 1.7E-14 |
| 9 | g3557.t8 | ProSiteProfiles | PS50802 | OTU domain profile. | 189 | 313 | 16.214 |
| 4 | g3557.t8 | SUPERFAMILY | SSF54001 | Cysteine proteinases | 172 | 314 | 3.83E-36 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004843 | thiol-dependent deubiquitinase | MF |
| GO:0016579 | protein deubiquitination | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
