General information for g3599.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative NF-kappa-B inhibitor cactus.
Parent gene
- The parent gene g3599 is located on chr_3: 26653924..26654420 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3599 | g3599.t2 | isoform | g3599.t2 | 26652230 | 26653924 |
| chr_3 | g3599 | g3599.t2 | exon | g3599.t2.exon1 | 26652230 | 26653924 |
| chr_3 | g3599 | g3599.t2 | cds | g3599.t2.CDS1 | 26652791 | 26653924 |
| chr_3 | g3599 | g3599.t2 | TSS | g3599.t2 | 26654420 | 26654420 |
| chr_3 | g3599 | g3599.t2 | TTS | g3599.t2 | NA | NA |
Sequences
>g3599.t2 Gene=g3599 Length=1695
ATGTCGACACAACACGAAATAAGCGAGAATTTAAAGAAAAATATTGACGATCAGACATCA
TCGTACAGTGATTCTGGATTCATTACAGAAAGCTGTGAATTGACTGGCGACCAAAGTAAA
GAAATTAGTGCTGATATCAAGCGCGAAGAAACTGATATGACGGTGAAAAAAACAATAAAA
ACGACGATGACGGAAAATCCCAAGTCCCAATCTAAAGATAGCGAAACAGAAAATTACACG
GATAGTGGTATTGTTTCATGTTCAAATATATCATCACAGCAGCAAAATCAGCAGTCCATA
TTAATTACGGATCTGGAGCCTTATGTGCCTACATATATGATAAGAAGGAAAATTCAGCAG
CCACAGCATCATAATTTGCAAATCGAAAAATATTTTGAGCAAGATGAAGATGGCTATACT
AAGCTTCATATTGCGATTTTACACAATATTGAACCAGCAATTCAAATTCTCATCAATTGT
GCACCAAATCCACATTATTTGAATATTAAAAATTATTTTGGGCAAACTGCACTTCATTTG
GCAGTGTTGCAGCAAAGTCATACAACAGTTCATAAACTTATTAACGCAGGTGCTGATATC
AATGCCCGTGATAATAGATGTAATTCACCATTACAATTAGCAGTACTTAATCAGGATATA
AATTGCGTTGCCGTTATTCTATCTGCCATTTATCCAGATGAAAAAAAAGCCCCAATTGCC
AATTTAGAGCAATGGAATTTTGACGGCGAAACATGCTTTTATGTTGCTTGCAAACAGAGA
AATATTCCAATTATCAAGCTTCTTGCTAAAACTGGAGCAAATGTGAATGCTAGAGAAGGA
AGATCCGGCTATTCGCCGTTGCATTTAGCAGTTGAAACACATGCAAACGAAGTCATTAAA
TTTCTATGCGAAAGCTGTATTGATTCAATAGACCTTGATTGTGAAACTTACGGTGGCTTG
ACAGCATTTCAATTGTCTTTACTCACTAATCAAGAACCTTTGGCAGAATATTTGATGAAA
AAAGGCGCTACACCTTATTTCATGGACAGCGATGAAGAACTTGAAGACAGCGATAGTGAA
GAAGATGAAAAAAATCAGTTGGTAAATAAGATTGCTGAAATAGCTGTTAATTAGTAACAA
TAAACATAATCACATTTTAAAGTAATCTATGATGATGATGTCCCTCCTTCATACTGTAAA
AGAAAGATGATAAAGCTGGCTGTATGAAAGAAGGAGCAAGAGAGGGAAATGGATTAATTT
TTCCAATTAATTCGTTGTTGTTTTTGTATTATCTATAATAATAAATTTATATTATTACAT
AATTAAAAATAATAGACTCTTTAAAAGAGTTCCAAATAAGAGAAATGAAGAAGTTATATA
TAATTTTTCAAATTGTTATCAAATCTATAAATAAATTTTAATTAAATTTTAACAAATCAA
CTATCTAAAGTTTTTTTAAAAAGAACAATAGGAAAAGTACTTTATTTCAGTAACTAAATA
TTACATTTATTTAATGAGTAAAGAATAAAAATAACCAACATTTTCATTCAGAATTGAAAC
AACATTTTAATAATAACTTATACCATATATAGTCAAAAGATTACCTTTCCATTTGGAAAT
TTTATTTTTATTTTTTGTGTAAAAGAAATAGCAACAAAAATGTATTGGGGAAATTCCCTG
CCTCTCGGACTCACT
>g3599.t2 Gene=g3599 Length=377
MSTQHEISENLKKNIDDQTSSYSDSGFITESCELTGDQSKEISADIKREETDMTVKKTIK
TTMTENPKSQSKDSETENYTDSGIVSCSNISSQQQNQQSILITDLEPYVPTYMIRRKIQQ
PQHHNLQIEKYFEQDEDGYTKLHIAILHNIEPAIQILINCAPNPHYLNIKNYFGQTALHL
AVLQQSHTTVHKLINAGADINARDNRCNSPLQLAVLNQDINCVAVILSAIYPDEKKAPIA
NLEQWNFDGETCFYVACKQRNIPIIKLLAKTGANVNAREGRSGYSPLHLAVETHANEVIK
FLCESCIDSIDLDCETYGGLTAFQLSLLTNQEPLAEYLMKKGATPYFMDSDEELEDSDSE
EDEKNQLVNKIAEIAVN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g3599.t2 | Gene3D | G3DSA:1.25.40.20 | - | 131 | 345 | 1.2E-46 |
| 12 | g3599.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 28 | - |
| 11 | g3599.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 57 | 79 | - |
| 3 | g3599.t2 | PANTHER | PTHR46680 | NF-KAPPA-B INHIBITOR ALPHA | 11 | 363 | 2.1E-59 |
| 2 | g3599.t2 | Pfam | PF12796 | Ankyrin repeats (3 copies) | 135 | 204 | 4.7E-9 |
| 1 | g3599.t2 | Pfam | PF13637 | Ankyrin repeats (many copies) | 251 | 302 | 1.2E-5 |
| 14 | g3599.t2 | ProSiteProfiles | PS50297 | Ankyrin repeat region circular profile. | 137 | 350 | 37.056 |
| 16 | g3599.t2 | ProSiteProfiles | PS50088 | Ankyrin repeat profile. | 173 | 205 | 13.063 |
| 15 | g3599.t2 | ProSiteProfiles | PS50088 | Ankyrin repeat profile. | 248 | 280 | 10.766 |
| 7 | g3599.t2 | SMART | SM00248 | ANK_2a | 137 | 166 | 180.0 |
| 9 | g3599.t2 | SMART | SM00248 | ANK_2a | 173 | 202 | 1.7E-4 |
| 10 | g3599.t2 | SMART | SM00248 | ANK_2a | 206 | 237 | 330.0 |
| 8 | g3599.t2 | SMART | SM00248 | ANK_2a | 248 | 277 | 3.4E-4 |
| 6 | g3599.t2 | SMART | SM00248 | ANK_2a | 282 | 312 | 0.064 |
| 5 | g3599.t2 | SMART | SM00248 | ANK_2a | 318 | 347 | 25.0 |
| 4 | g3599.t2 | SUPERFAMILY | SSF48403 | Ankyrin repeat | 137 | 361 | 6.8E-53 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K04734 (NF-kappa-B inhibitor alpha)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
