General information for g3744.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Acetylcholinesterase.
Parent gene
- The parent gene g3744 is located on chr_3: 27781317..27784967 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g3744 | g3744.t1 | TTS | g3744.t1 | 27773833 | 27773833 |
| chr_3 | g3744 | g3744.t1 | isoform | g3744.t1 | 27774525 | 27781317 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon1 | 27774525 | 27774658 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS1 | 27774525 | 27774658 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon2 | 27774732 | 27774887 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS2 | 27774732 | 27774887 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon3 | 27774974 | 27774990 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS3 | 27774974 | 27774990 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon4 | 27775067 | 27775230 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS4 | 27775067 | 27775230 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon5 | 27775664 | 27775816 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS5 | 27775664 | 27775816 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon6 | 27778581 | 27778723 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS6 | 27778581 | 27778723 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon7 | 27778798 | 27779005 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS7 | 27778798 | 27779005 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon8 | 27779063 | 27779250 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS8 | 27779063 | 27779250 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon9 | 27779995 | 27780090 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS9 | 27779995 | 27780090 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon10 | 27780154 | 27780331 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS10 | 27780154 | 27780331 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon11 | 27780388 | 27780636 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS11 | 27780388 | 27780636 |
| chr_3 | g3744 | g3744.t1 | exon | g3744.t1.exon12 | 27781087 | 27781317 |
| chr_3 | g3744 | g3744.t1 | cds | g3744.t1.CDS12 | 27781087 | 27781317 |
| chr_3 | g3744 | g3744.t1 | TSS | g3744.t1 | NA | NA |
Sequences
>g3744.t1 Gene=g3744 Length=1917
ATGTTGAAATTTTATTATGTGCTATGGTTTTGTGCAGTGCTAAGTGTAATATGTCAAGTG
CATGGAATTATCGATAGATTAGTAGTGCAGACAACAAGTGGTCCTATACGCGGCTCTTCA
ACTTTCGTCGAGGGCCATGAAGTGCACATATTCCTCGGCATTCCATTTGCTAAGCCACCC
ATTGATAGTCTAAGATTTAGAAAGCCTGTTCCTAGCGAGCCATGGCATGGGGTTTACGAT
GCAACGCGATTGCCGCCTTCGTGCATTCAAGAACGATATGAGTACTTTCCAGGCTTCTCG
GGTGAGGAGATGTGGAATCCAAACACAAATGTTAGCGAGGATTGTCTCTATTTGAACATT
TGGGCGCCAGCAAAGGCTCGTCTTCGGCACGAGCGAGTGCATGAAAATGGCAATGGCAAA
CATGGAAATCATTTTGAAAGTACAAACGATCATCATGAACACGAAGAAGGACTACCTATG
CTAATATGGATCTATGGTGGTGGTTACATGAGTGGCACATCAACTCTTGACATTTACAAT
GCAAAAACACTCGCAGCAGTTGGAAATGTTATTGTTGCATCGATGAACTATCGTGTCGGT
GCATTTGGTTTTTTATATTTGGCGCCGTTTTTGCCCGGGCATGAGGATGAGGCACCAGGT
AATCAAGGCATGTGGGATCAAGCATTAGCTATTCGATGGTTGAAGGACAATGCTCGTTCA
TTTGGTGGAGATCCTAGTTTGATTACACTTTTTGGTGAAAGCGCTGGTGGTGGAAGTGTC
AGTCTACATTTGCTATCACCAGTCACTCGTGGATTAGTTACTCGTGGAATTTTGCAATCA
GGCACGTTGAATGCACCATGGAGTCATATGACAGGCGAGAGAGCTGCACAAATTGGTGAA
ATGTTAGTTGAAGATTGTAACTGTAATGCAACTAATTTGAAGGATTCTCCACGTGCTGTT
ATGAATTGCTTAAGAACGGTTGATGCAAAGACAATCTCAGTACAACAGTGGAACTCTTAT
TCTGGCATTTTAGGTTTTCCATCAGCGCCCACTATTGATGGTGACTTTATGCCTTCTGAT
CCCACGACGATGCTTGCTCGAGCTAATCTCGAAAATGTTGACATACTTGTTGGTAGCAAT
CGAGACGAAGGAACATATTTTCTCTTATACGATTTTATTGACTATTTCGATCGCTCTTTG
CCAACACAATTATCAAGAGAGAAATTTTTAGACATCATGAACACAATTTTTAGCAAAGCA
TCACAACCTGAACGTGAAGCTATCATTTTTCAGTATACAGGATATGAGGCAGCCAACGAT
GACTACCAAAATCAGCAGCAAGTCGCGAGAGCCGTTGCCGATCATTTTTTCATCTGTCCA
ACAAATGAATTTGCGATTGGACTTGCTGACCGTGGTGCAAATGTTTATTACTACTATTTT
ACACATCGTACAAGTACATCTTTATGGGGTGAATGGATGGGAGTTTTGCATGCAGATGAA
ATCGAATACATCTTTGGACATCCTCTTAATAGTACATTGCAGTATCGTCAACGAGAAAGA
GACTTGAGTCGTATGATGGTTCAATCTGTAACAAAATTTGCAAGAACTGGAAATCCATCG
TTAGACGGAATTCATTGGCCTCAATACAAAGCCGACAATCCAATTTATTTTATATTTAAT
GCAGAAGGCGAAGAAGATATGCGAGCAAATAAGTATGGTCGTGGTCCAGCTGCAACTTCA
TGCGCTTTTTGGAATGATTTTCTACCAAGACTAAGAAACTGGCTGCCTACATCTGTGCCA
TGTGATAATGGGGGTACACCTAAAGGCCACACTTCCCGTGGACTCATTTTGCACGTTAGT
CGGATTCTCGTAATGGTTGCTGCACTGTTGCTTGTGTCATTCTATCGAGTGTTGTAA
>g3744.t1 Gene=g3744 Length=638
MLKFYYVLWFCAVLSVICQVHGIIDRLVVQTTSGPIRGSSTFVEGHEVHIFLGIPFAKPP
IDSLRFRKPVPSEPWHGVYDATRLPPSCIQERYEYFPGFSGEEMWNPNTNVSEDCLYLNI
WAPAKARLRHERVHENGNGKHGNHFESTNDHHEHEEGLPMLIWIYGGGYMSGTSTLDIYN
AKTLAAVGNVIVASMNYRVGAFGFLYLAPFLPGHEDEAPGNQGMWDQALAIRWLKDNARS
FGGDPSLITLFGESAGGGSVSLHLLSPVTRGLVTRGILQSGTLNAPWSHMTGERAAQIGE
MLVEDCNCNATNLKDSPRAVMNCLRTVDAKTISVQQWNSYSGILGFPSAPTIDGDFMPSD
PTTMLARANLENVDILVGSNRDEGTYFLLYDFIDYFDRSLPTQLSREKFLDIMNTIFSKA
SQPEREAIIFQYTGYEAANDDYQNQQQVARAVADHFFICPTNEFAIGLADRGANVYYYYF
THRTSTSLWGEWMGVLHADEIEYIFGHPLNSTLQYRQRERDLSRMMVQSVTKFARTGNPS
LDGIHWPQYKADNPIYFIFNAEGEEDMRANKYGRGPAATSCAFWNDFLPRLRNWLPTSVP
CDNGGTPKGHTSRGLILHVSRILVMVAALLLVSFYRVL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g3744.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 23 | 600 | 3.5E-191 |
| 2 | g3744.t1 | PANTHER | PTHR43918:SF5 | CHOLINESTERASE | 11 | 595 | 8.5E-238 |
| 3 | g3744.t1 | PANTHER | PTHR43918 | ACETYLCHOLINESTERASE | 11 | 595 | 8.5E-238 |
| 8 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 30 | 35 | 7.6E-17 |
| 9 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 44 | 52 | 7.6E-17 |
| 10 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 73 | 77 | 7.6E-17 |
| 7 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 91 | 96 | 7.6E-17 |
| 18 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 93 | 107 | 4.5E-32 |
| 6 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 125 | 130 | 7.6E-17 |
| 17 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 165 | 194 | 4.5E-32 |
| 11 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 180 | 189 | 7.6E-17 |
| 5 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 232 | 236 | 7.6E-17 |
| 12 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 330 | 336 | 7.6E-17 |
| 15 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 384 | 392 | 4.5E-32 |
| 14 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 451 | 459 | 4.5E-32 |
| 16 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 489 | 496 | 4.5E-32 |
| 13 | g3744.t1 | PRINTS | PR00878 | Cholinesterase signature | 497 | 509 | 4.5E-32 |
| 4 | g3744.t1 | PRINTS | PR00880 | Insect acetylcholinesterase signature | 543 | 550 | 7.6E-17 |
| 1 | g3744.t1 | Pfam | PF00135 | Carboxylesterase family | 27 | 584 | 8.0E-143 |
| 24 | g3744.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 25 | g3744.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 26 | g3744.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 18 | - |
| 28 | g3744.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 22 | - |
| 23 | g3744.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 614 | - |
| 27 | g3744.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 615 | 635 | - |
| 22 | g3744.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 636 | 638 | - |
| 31 | g3744.t1 | ProSitePatterns | PS00941 | Carboxylesterases type-B signature 2. | 113 | 123 | - |
| 32 | g3744.t1 | ProSitePatterns | PS00122 | Carboxylesterases type-B serine active site. | 241 | 256 | - |
| 19 | g3744.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 26 | 593 | 1.09E-150 |
| 20 | g3744.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 30 | g3744.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 24 | - |
| 29 | g3744.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 615 | 637 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004104 | cholinesterase activity | MF |
| GO:0003990 | acetylcholinesterase activity | MF |
| GO:0001507 | acetylcholine catabolic process in synaptic cleft | BP |
KEGG
Orthology
- This transcript has been annotated with K01049 (acetylcholinesterase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
